LOCUS       sequence-407166-       12986 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Not1 expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-lambda-N-HA-NOT1 isoform C (JM155)
FEATURES             Location/Qualifiers
     source          1..12986
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     13..31
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        95..2604
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2624..2689
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             2714..2740
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10331..10372
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             10382..10399
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10422..10439)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10472..10606
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10496..10515)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     10550..10569
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(11207..11795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11287..11306)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11966..12757,12758..12826))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12589..12608
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12827..12931)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattctatat
       61 tctaaaaaca caaatgatac ttctaaaaaa aaatcatgaa tggcatcaac tctgaatcaa
      121 atctttgcag atgcacctac ttctcatttc cactgtcaca tcatttttcc agatctcgct
      181 gcctgttatg tggcccacaa accaagacac gttttatggc cattaaagct ggctgatcgt
      241 cgccaaacac caaatacata atgaatatgt acacattcga gaaagaagcg atcaaagaag
      301 cgtcttcggg cggagtagga gaatgcggag gagaaggaga acgagctgat ctagtatctc
      361 tccacaatcc aatgccaact gaccaactgg ccatattcgg agcaatttga agccaatttc
      421 catcgcctgg cgatcgctcc attcttggct atatgttttt caccgttacc cggggccatt
      481 ttcaaagact cgtcggcaag ataagattgt gtcactcgct gtctctcttc atttgtcgaa
      541 gaatgctgag gaatttcgcg atgacgtcgg cgagtatttt gaagaatgag aataatttgt
      601 atttatacga aaatcagtta gtggaatttt ctacaaaaac atgttatcta tagataattt
      661 tgttgcaaaa tatgttgact atgacaaaga ttgtatgtat atacctttaa tgtattctca
      721 ttttcttatg tatttataat ggcaatgatg atactgatga tattttaaga tgatgccaga
      781 ccaaaaggct tgaatttctg cgtcttttgc cgaacgcagt gcatgtgcaa ttgttgtttt
      841 ttggaatatt caattttcgg actgtccgct ttgatttcag tttcttggct tattcaaaaa
      901 gcaaagtaaa gccaaaaaag cgagatggca ataccaaatg cggcaaaacg gtagtggaag
      961 gaaaggggtg cggggcagcg gaaggaaggg tggggcgggg cgtggcgggg tctgtggctg
     1021 ggcgcgacgt caccgacgtt ggagccactc ctttgaccat gtgtgcgtgt gtgtattatt
     1081 cgtgtctcgc cactcgccgg ttgttttttt ctttttatgc tgcgctctct ctagcgccat
     1141 ctcgcttacg catgctcaac gcaccgcatg ttgccgtttc cttttatgcg tcattttggc
     1201 tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa taaataagtc
     1261 tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa aagcaacaac
     1321 acaggtggct ttccctcttt cactttttgt ttgcaagccg cgtgcgagca agacggcacg
     1381 accggcaaac gcaattacgc tgacaaagag cagacgaagt tttggcgaaa aacatcaagg
     1441 cgcctgatac gaatgcattt gcaataacaa ttgcgatatt taatattgtt tatgaagctg
     1501 tttgacttca aaacacacaa aaaaaaaaat aaaacaaatt atttgaaaga gaattaggaa
     1561 tcggacgctt atcgttaggg taacaacaag aaatgcttac tgagtcacag cctctggaaa
     1621 actgccgcaa gccagagaga gagagaaaaa gagggagagc agcttagacc gcatgtgctt
     1681 gtgtgtgagg cgtctctctc ttcgtctctg ttgcgcaaac gcatagactg cactgaaaaa
     1741 atcgattacc tattttttat gaatgaatat ttgcactatt actattcaaa actattaaga
     1801 tagcaatcac attcaatagc caaatactat accacctgag cgatgcaacg aaatgatcaa
     1861 tttgagcaaa aatgctgcat atttaggacg gcatcattat agaaatgctt cttgctgtgt
     1921 acttttctct cgtctggcag ctgtttcgcc gttattgtta aaaccggctt aagttaggtg
     1981 tgttttctac gactagtgaa tgccctacta gaagatgtgt gttgcacaaa atgtccctgg
     2041 aataaccaat ttgaagtgca gatagcagta aacgtaagct aatatgaata ttatttaact
     2101 gtaatgtttt aatatcgctg gacattacta ataaacccac tataaacaca tgtacatatg
     2161 tatgttttgg catacaatga gtagttgggg aaaaaatgtg taaaagcacc gtgaccatca
     2221 cagcataaag ataaccagct gaagtatcga atatgagtaa cccccaaatt gaatcacatg
     2281 ccgcaactga taggacccat ggaagtacac tcttcatggc gatatacaag acacacacaa
     2341 gcacgaacac ccagttgcgg aggaaattct ccgtaaatga aaacccaatc ggcgaacaat
     2401 tcatacccat atatggtaaa agttttgaac gcgacttgag agcggagagc attgcggctg
     2461 ataaggtttt agcgctaagc gggctttata aaacgggctg cgggaccagt tttcatatca
     2521 ctaccgtttg agttcttgtg ctgtgtggat actcctcccg acacaaagcc gctccatcag
     2581 ccagcagtcg tctaatccag agaccccgga tcggggtacc aacatggacg cacaaacacg
     2641 acgacgtgag cgtcgcgctg agaaacaagc tcaatggaaa gctgcaaacg gtggatctcc
     2701 tccacatatg gcttacccat acgatgttcc agattacgct cctccaagct tgaattctgc
     2761 agatatgaac gtagagagcc aactgaagac accgccaacg cacattcgaa atctggtcaa
     2821 gcacgtgaac aagcgaaatt ttaatgagag cagcgagcaa ataaatcagt tcgttaaaga
     2881 gcacggcctg gaagcggatc gcagcagcct gcgccacctg ttctccgtga tcaacttcag
     2941 cgatctcgtg ccatcagtta cggtccagct gcaggcgaag ctcctgggca tccagttgga
     3001 gcgtcagctt cacagttcat cgtttgtgtc caacatctgc tacgcttttg accagttctt
     3061 cgccagcaac cagaagtccc tgaagcctgt tgcggtagcg gatctcgttg gccaggtcgc
     3121 ccgactgacc ggcatcaaca aggtttgtga gtgcgtcttt gccttggcct tgacccactc
     3181 ctcgtacaca gaactcaaac attctgccag gaacaaccta aaaggtagcc tcagcgaact
     3241 tatagactct tacttgggta ataaaggcac cggcccagcc gacagcggtc tccgggagat
     3301 ctccttcgat ctactgcagt atttgctgtg ctgccttagc gagtacgtac aaccgcaggt
     3361 ggaagcacag tttttgatca agcttcgtga ggagtttccg cgccaggcgg taccgctggt
     3421 tctagcgccg ttcctttacg gctcgacgac ggcgacgatt gcgggagcag gtgccagcga
     3481 aacggatgcg gaggccgaag cgacgactaa tagcaacagc tccagtttcg aagcagatgc
     3541 tttgaacgag gtgggaatcg aggatattta tgaccattta agcgagataa tattcaccaa
     3601 ccagggcaaa aataatatta tggacacatc ttggattaat ctaatccttg aaatcggtta
     3661 tgaatttaca tcgagcgttg aagagtgtaa gaatcatttg tgttcccgcg agcgagaacg
     3721 cgctgagctc cagtccaaag atgtcgccaa gatcgtgggt cttatgtgcc gacggcactc
     3781 ctccctgctc gattgtaatg tgaatctacc gacgccggcg aacttctggc ctggtcaggg
     3841 acagggcggt ggcagcaaca gcagtggctc ttcgcagaca caaatcactc cgcagcaaca
     3901 gaacccgggc agcagtaaca acaacgatgg cagcgatggc aattcgtcca gtgataagaa
     3961 ggacaagaag gagacgacgg aggccacgca aacttggaag ccagatgtct ttgtgcaggc
     4021 gctcaaggag gtggtgcctc agctcaactg gaaggacgtg tgcatggaac ttgatcatcc
     4081 cgagttcgtg ctgaaggatc gcatcggttt ggagcttttg ctcaccatcc ttcggctggc
     4141 cactggctct aacatatttc cacatccaga atgcatttac cgtcactggg caaacacgga
     4201 aggtcagctg tcgcttatcg cgacaatgct gaagaacccg gatctctttt ccttcgccga
     4261 ctttgtattt agccagccag cgttggatgt gcttaagacg gctccggatg cggacaataa
     4321 ggagatctcg tcctggaagt cgcttcacct ggtggaggtg ctgctctcca ttgcggacaa
     4381 gggatactat acgcaggtcc atgagctatt caagttccca gcgcagaact gtcccgatgt
     4441 gctatttttg gccttattga acacaagtcc gcctatgacg ccactgcgtc aggatctgtt
     4501 taaccaactg ataccaacgt tccttggcaa ccatcccaac tcgaacgtga tcttggccag
     4561 cgcctggagc tcgaacaact tccaacttcg ttccaatatc atgaatgcta tgtccgagtg
     4621 gtatctccga ggcaatgagt tcgatcaggt gaaactgtcg cgcatcctgg atcttgccca
     4681 ggatttaaaa gcgctatcag cgctgctcaa cgcccgttca tttttattca ttatcgacct
     4741 ggcttgcctt gcctctcggc gcgagtacct gaagctggaa aagtggctta ccgataaaat
     4801 tcgcgaacat ggagagcctt ttatgcaggc tataatcaag gttctgcacc gccgctgccc
     4861 gcaggttatc aacgccaaag tgccagagga tcagctgcct cccaagcagg cgcagctttt
     4921 gcccgagaca gttacaacca tgatcaactg tctgcaaacg tgcatcaata actgcatgca
     4981 gccggagatg gtcgaagtca tcatgcaaat gaccgcgaac gtggcgatta tggctaacaa
     5041 ggcgcgtgcc caacagcagc agcagccagg attggttccg ccgcctccac caacaattct
     5101 gcgcggccat cgtggtatgg atctgcctgg aggcatagtc cctccgccac cgcagcaacc
     5161 gttctccgga aacctcaacg cgcagatgtt tggaccagga atggatccgc taacgaatat
     5221 gtccaacaac ttagccggcc tcaacctcag cggcccgaac ggagcattta attttggaaa
     5281 catgctaact tctccatcgc gtctgatgac tccgggagcc aatccctatc cgctgaacct
     5341 catgcagatg ccgcaggctc cgccaccacc caacgtagga aacctaggcc gcatgctgcc
     5401 agggggtcca caacagcaga ctccaacgcc gactccaacg gcccccaatc caaacaaccc
     5461 tgtgatggcc gatctccaga tacctgtgtc aaaggaagtt gaagacgagg tcaactcata
     5521 ctttcagcgc atctacaacc accagccgaa tccaacgctg tccatcgatg aagtgctgga
     5581 cattttgcaa agatttaaag agtcaagtaa ccggcgcgag caggaagtct ttttgtgcat
     5641 gctgcgcaat ctgttcgagg aatatcgctt tttctgtcaa tacccagaga aagagctaca
     5701 gattactgcg cagctatttg gtggcattat tgatcgtaac ctggtgccca cttttgttgc
     5761 actgggcctt tctctgcgct gtgtcttgga tgccctccgc aagcccgatg gatctaagct
     5821 ttactatttc ggtgtgacgg cactggacag attcagaact cgattgcaca cctacaacaa
     5881 atactgcgag catatccgct ccattcctca cttctctgac ttcccgccgc accttattca
     5941 gtatgtggaa tacggaatgc acggccagga gccgccgccg cagaagttga ttggactcag
     6001 caatacgatt ccttcggcga tatctagcgg accaggaacg gaaccaattt acaggaatag
     6061 ctccatgtta ggtaatatgc ccgctgccac cccgggatcg ggaccgaaat caaacgctgc
     6121 tgtatcgcat gccacgagaa tgaagtccat cgccaatgcc accaatatcg acacactttt
     6181 ggtggccaac caggaagaga aagtgactgt gccaccagag cctgtgcagg acaaaactgc
     6241 ctttattttc aacaacttga gccagctgaa cataccgcag aagtgcgatg agataaagga
     6301 gattatgacc aaggagtatt ggccgtggct agcgcaatat ctggtcctca aacgcgcatc
     6361 aatggagttc aacttccaca cactatatta caactttttg gatgccctta aaaacggcga
     6421 gatcaaccga ttcgtaacca aagagaccct acgcaacatc aaggtactct tgcgctccga
     6481 taagggagtt attaatttct ccgatcgaag tctgttgaag aacctcggac actggttggg
     6541 catgatgacc ttaggtcgca atcgccccat cctgcagttg gatctggatc ttaaatccct
     6601 tttggctgag gcttaccaca agggccagca ggaactgctt tttgtggttc ctttcgtagc
     6661 caagatcctt gagtcatccg ccaagtctcg catcttccga tcgcccaatc catggacaat
     6721 gggtatcatg tacgtgcttg ctgagcttca ccaggagcca gaccttaagc tgaacctgaa
     6781 gtttgaaatc gaagtactct gcaaaacact taatctggag ttggccaagt tgcgacaagt
     6841 gatctacctg aaggatccca atcgaactca ccggattgaa gagcagatgt cgcaaccaaa
     6901 accgaaacaa cttgagccag tggcttctgc gccagctcta ccgcgtgaac agcaatcccc
     6961 ggcacaacct ccgccaccac cacaacagca gcaaccaccg cagcagcagg tacctccacc
     7021 gccatcctct gcggacgtgg acgcccaaaa tgcggccgct atgatgatgg cagcgggcgg
     7081 agctaatagt actcctggat cggtatcttc gcccaatcta cccaccgatt ccagccaggt
     7141 ggcactaccg ccaccagagc cgcgctattc atacgtggac gtaaacgtga gcaacttcca
     7201 gcttattggt cagcagttgg tactgccacc caatactccg ttcctgcacg ccaatcccgg
     7261 aatcaaacac atggtcgtca acgccgtgga gcgcacgata accgactggt tgcagcctat
     7321 tgtggaccga agcatccgca ttgcctgtgc caccaccgag cagatcatcc gcaaggactt
     7381 cgccctggat gctgacgaga accgaatgcg cactgccgct catcagatgg tacgaaacct
     7441 tgccgccggc atggccatga ttaccggtaa ggatgagata gcgcgtgcta ttagccagaa
     7501 tctgcacaag gccttgctgt cgggtctaaa tgggatgccc agcatggctg agatccaagc
     7561 tgctgctatg cagttagcca gcgaaaatgt tgagctagtt tgcgctttca ttcagaaaac
     7621 atctgctgaa aaggcagccg cagagatcga taggcgtcta agcaccgatt tcgagaccag
     7681 aaaaattgct cgtgaagagg gcaaccgctt tgtggacgcc cagatcctca cctatcaaca
     7741 ggagcgtctt ccagaagcag tgcgcatcaa ggtgggcgcg gctccagcta cactttatgc
     7801 tgtgtactcg gagttcgcta gaagcatacc tggcttccag cagatgagcg atcgcgatat
     7861 tgccctgttt gtgcccaagc cgacggattt gtctcagcca aatgtgtttg ctaatgatga
     7921 tagcatggta tacggggaac tggctagcaa aatggaggcc ttcatgaaca cggcaatcgg
     7981 agtgccgaca ctccagatcc aggctagcaa gatgcacatg ctactcaacg ccctgatagc
     8041 gacgcgtcga ctgcgtgacc aagagtctgc ctttaatctg ctgacccgcg cggtcgaggg
     8101 tttgaccgaa ggattggtca atatgcatga aaacatggag caaatgaaga tgtaccaaaa
     8161 catccatctc cgtatccttg gcttgctgaa caacagtttc ggtgctccaa acacagagcg
     8221 agcggtaaca aagtgtttct tcgatatccg agaggaggtg cgatataacg tggaggcagc
     8281 tcgcgctttg attacgtcgc actttgtcaa tctgaatcag tttgacggaa tgctacgcga
     8341 ctgcatggac aacggaaaca attatgtggc tatatcgttc ggtatcgcac tgctggagcg
     8401 cctcatcatg gacgatcgag tgattaatat tgtttcggac aacgaattca tggcaactgt
     8461 tgagctgcta ggtaggctca ctcagcatcg ccatcggtat ccagaatgca ttgtgaatgc
     8521 tattgatacg ctctggagcg gaaactttaa ctcgagcagc gactacagcc cgttcaacgg
     8581 caatgatcgt tacttgtcgg gagcctccca ttacatccac tccggcatgc atcacgtaag
     8641 gtcatgcgat acagatgatc cgccgggctt acaagagaag acggagtttc tgcttaagga
     8701 ttgggttgca ctgtacacgc aacagaacca gcagtcaacg cgcgatgcac gcaacttcag
     8761 tgcatttgtt cagaagatga acacatatgg tatcctgaaa acggacgact tgatcacccg
     8821 ttttttccgc caggccacac acatttgcac ggatgtggtg tacagaatgt tcgccgagcc
     8881 gagtttacca atcaaccagg ccaaaaacaa gatattccag tggatcgatg cgttcgtcca
     8941 tctgattgca atgctcgtgc gacactcagg cgaggcaggt aatccaacca ccaagatcaa
     9001 cctgttaaac aaggtgctgg gcattgtgct gggaacgctg attaaggacc atgagatgcg
     9061 tggcgttagc ttccagcagg ttggctacca tcgcttcttc atgatgctgt tcatggagct
     9121 gtgcacggcc gatgtgattc tcgagtccct gatgcacagt attgtgtcgg ccttcgccta
     9181 cacatatcac ttgttgaatc ccagcgtggc tccgggattc tgctttgctt ggttggagct
     9241 catttcgcat cgcgtcttcc tgggccgcat cctggtccaa attcccgggc aaaagggctg
     9301 gccactttac gctcaattgc tgcaggacct ttttaaatat cttgcgccct tcttacgcaa
     9361 cactgagctc ggcaaaccag tccagctttt gtacaagggg atgctacgtg ttctgctcgt
     9421 tctgcttcac gacttcccgg agttcttgtg cgactatcat tttgggttct gtgacaccat
     9481 cccgcccaat tgcgtccaga tgcgtaacat catcctgtcg gcgtttccgc gcaacatgcg
     9541 cttgcccgat cccttcacgc ccaacctaaa agtggacatg ctgtcggaca gtagtaatgc
     9601 gcctaaggtg ctcagtagct acattatgaa catccagccc gcgaacttta agaaggattt
     9661 ggactcgtac ctcaaggccc gggcgccagt tacttttcta tcggaattac gtggtcactt
     9721 gcaggtgaca agcgagccag gaactcgtta caacatggcg ctgatgaatg ctctggtcat
     9781 gtatgtggga actcaggcga ttgctttgat cagaaacaaa aactttgtgc ccaacacctc
     9841 gaacatagca cacagcgccc acatggatat cttccagaat cttgctgttg acttggatac
     9901 ggagggtcgc tatctgttct tgaatgcgat tgcgaaccag ttgcgctatc ccaacagcca
     9961 cacgcactac ttcagctgtg cggtgctgca tctgtttgcc gaggccaact cggaggcaat
    10021 tcaggagcag atcactcgcg tacttctgga acgattgatc gtgaaccgcc cgcatccttg
    10081 gggactgttg atcactttta ttgagctgat caaaaatccc atctacaagt tctgggacca
    10141 tgactttgtg cactgtgcgc cagagatcac caagctcttt gaatcggtgg cccgttcctg
    10201 tctggccaag tcgaacgtca cccagcagtt gaacatgccc gtcgtcgatg gcgagggcca
    10261 ggaggtagcc accatcaact gaatccagca cagtggcggc cgctcgagtc tagagggccc
    10321 gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg
    10381 tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctaa
    10441 ggcctgagct cgctgatcag cctcgatcga ggatccagac atgataagat acattgatga
    10501 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
    10561 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
    10621 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
    10681 cctctacaaa tgtggtatgg ctgattatga tcagtcgacc gatgcccttg agagccttca
    10741 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg
    10801 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg
    10861 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct
    10921 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc
    10981 aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga
    11041 cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc
    11101 ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag
    11161 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    11221 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    11281 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    11341 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    11401 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    11461 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    11521 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    11581 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    11641 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11701 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11761 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    11821 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    11881 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    11941 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    12001 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    12061 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    12121 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    12181 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    12241 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
    12301 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    12361 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    12421 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    12481 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    12541 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
    12601 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    12661 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    12721 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    12781 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    12841 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    12901 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    12961 tctaagaaac cattattatc atgaca
//
