LOCUS       sequence-407177-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VEGFR3 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-VEGFR3-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      192..620
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(279..298)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     489..510
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(629..649)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        634..963
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      814..949
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     876..895
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1030..1824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1084..1103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1694..1713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2000..2121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2037..2056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2091..2110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2170..2186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2170..2186)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2183..2205)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2194..2210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2218..2224,2225..2242,2243..2248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2263..2284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2401..2418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2572..3160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2652..2671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3331..4122,4123..4191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3954..3973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4192..4296)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4371..4390)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4562..4941
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4942..5145
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5096..5116
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5190..5209
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5190..5208
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9455..9496
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             9506..9523
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(9546..9563)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9552..9630,1..146)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
       61 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
      121 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
      181 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      241 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      301 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      361 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      421 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      481 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      541 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      601 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      661 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      721 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      781 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      841 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      901 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      961 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     1021 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     1081 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     1141 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     1201 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     1261 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     1321 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     1381 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1441 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1501 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1561 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1621 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1681 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1741 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1801 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     1861 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     1921 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1981 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     2041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2101 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     2161 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     2341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2761 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3001 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4321 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     4381 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     4441 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     4501 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     4561 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     4621 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     4681 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     4741 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     4801 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     4861 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     4921 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4981 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     5041 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     5101 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga
     5161 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
     5221 ggctagttaa gcttggtacc gagctcggat ccagtaccct tcaccagcag aagggccgtc
     5281 aaggcccacc atgcagcggg gcgccgcgct gtgcctgcga ctgtggctct gcctgggact
     5341 cctggacggc ctggtgagtg gctactccat gccccccccg accttgaaca tcacggagga
     5401 gtcacacgtc atcgacaccg gtgacagcct gtccatctcc tgcaggggac agcaccccct
     5461 cgagtgggct tggccaggag ctcaggaggc gccagccacc ggagacaagg acagcgagga
     5521 cacgggggtg gtgcgagact gcgagggcac agacgccagg ccctactgca aggtgttgct
     5581 gctgcacgag gtacatgcca acgacacagg cagctacgtc tgctactaca agtacatcaa
     5641 ggcacgcatc gagggcacca cggccgccag ctcctacgtg ttcgtgagag actttgagca
     5701 gccattcatc aacaagcctg acacgctctt ggtcaacagg aaggacgcca tgtgggtgcc
     5761 ctgtctggtg tccatccccg gcctcaatgt cacgctgcgc tcgcaaagct cggtgctgtg
     5821 gccagacggg caggaggtgg tgtgggatga ccggcggggc atgctcgtgt ccacgccact
     5881 gctgcacgat gccctgtacc tgcagtgcga gaccacctgg ggagaccagg acttcctttc
     5941 caaccccttc ctggtgcaca tcacaggcaa cgagctctat gacatccagc tgttgcccag
     6001 gaagtcgctg gagctgctgg taggggagaa gctggtcctg aactgcaccg tgtgggctga
     6061 gtttaactca ggtgtcacct ttgactggga ctacccaggg aagcaggcag agcggggtaa
     6121 gtgggtgccc gagcgacgct cccagcagac ccacacagaa ctctccagca tcctgaccat
     6181 ccacaacgtc agccagcacg acctgggctc gtatgtgtgc aaggccaaca acggcatcca
     6241 gcgatttcgg gagagcaccg aggtcattgt gcatgaaaat cccttcatca gcgtcgagtg
     6301 gctcaaagga cccatcctgg aggccacggc aggagacgag ctggtgaagc tgcccgtgaa
     6361 gctggcagcg taccccccgc ccgagttcca gtggtacaag gatggaaagg cactgtccgg
     6421 gcgccacagt ccacatgccc tggtgctcaa ggaggtgaca gaggccagca caggcaccta
     6481 caccctcgcc ctgtggaact ccgctgctgg cctgaggcgc aacatcagcc tggagctggt
     6541 ggtgaatgtg cccccccaga tacatgagaa ggaggcctcc tcccccagca tctactcgcg
     6601 tcacagccgc caggccctca cctgcacggc ctacggggtg cccctgcctc tcagaatcca
     6661 gtggcactgg cggccctgga caccctgcaa gatgtttgcc cagcgtagtc tccggcggcg
     6721 gcagcagcaa gacctcatgc cacagtgccg tgactggagg gcggtgacca cgcaggatgc
     6781 cgtgaacccc atcgagagcc tggacacctg gaccgagttt gtggagggaa agaataagac
     6841 tgtgagcaag ctggtgatcc agaatgccaa cgtgtctgcc atgtacaagt gtgtggtctc
     6901 caacaaggtg ggccaggatg agcggctcat ctacttctat gtgaccacca tccccgacgg
     6961 cttcaccatc gaatccaagc catccgagga gctactagag ggccagccgg tgctcctgag
     7021 ctgccaagcc gacagctaca agtacgagca tctgcgctgg taccgcctca acctgtccac
     7081 gctgcacgat gcgcacggga acccgcttct gctcgactgc aagaacgtgc atctgttcgc
     7141 cacccctctg gccgccagcc tggaggaggt ggcacctggg gcgcgccacg ccacgctcag
     7201 cctgagtatc ccccgcgtcg cgcccgagca cgagggccac tatgtgtgcg aagtgcaaga
     7261 ccggcgcagc catgacaagc actgccacaa gaagtacctg tcggtgcagg ccctggaagc
     7321 ccctcggctc acgcagaact tgaccgacct cctggtgaac gtgagcgact cgctggagat
     7381 gcagtgcttg gtggccggag cgcacgcgcc cagcatcgtg tggtacaaag acgagaggct
     7441 gctggaggaa aagtctggag tcgacttggc ggactccaac cagaagctga gcatccagcg
     7501 cgtgcgcgag gaggatgcgg gacgctatct gtgcagcgtg tgcaacgcca agggctgcgt
     7561 caactcctcc gccagcgtgg ccgtggaagg ctccgaggat aagggcagca tggagatcgt
     7621 gatccttgtc ggtaccggcg tcatcgctgt cttcttctgg gtcctcctcc tcctcatctt
     7681 ctgtaacatg aggaggccgg cccacgcaga catcaagacg ggctacctgt ccatcatcat
     7741 ggaccccggg gaggtgcctc tggaggagca atgcgaatac ctgtcctacg atgccagcca
     7801 gtgggaattc ccccgagagc ggctgcacct ggggagagtg ctcggctacg gcgccttcgg
     7861 gaaggtggtg gaagcctccg ctttcggcat ccacaagggc agcagctgtg acaccgtggc
     7921 cgtgaaaatg ctgaaagagg gcgccacggc cagcgagcac cgcgcgctga tgtcggagct
     7981 caagatcctc attcacatcg gcaaccacct caacgtggtc aacctcctcg gggcgtgcac
     8041 caagccgcag ggccccctca tggtgatcgt ggagttctgc aagtacggca acctctccaa
     8101 cttcctgcgc gccaagcggg acgccttcag cccctgcgcg gagaagtctc ccgagcagcg
     8161 cggacgcttc cgcgccatgg tggagctcgc caggctggat cggaggcggc cggggagcag
     8221 cgacagggtc ctcttcgcgc ggttctcgaa gaccgagggc ggagcgaggc gggcttctcc
     8281 agaccaagaa gctgaggacc tgtggctgag cccgctgacc atggaagatc ttgtctgcta
     8341 cagcttccag gtggccagag ggatggagtt cctggcttcc cgaaagtgca tccacagaga
     8401 cctggctgct cggaacattc tgctgtcgga aagcgacgtg gtgaagatct gtgactttgg
     8461 ccttgcccgg gacatctaca aagaccccga ctacgtccgc aagggcagtg cccggctgcc
     8521 cctgaagtgg atggcccctg aaagcatctt cgacaaggtg tacaccacgc agagtgacgt
     8581 gtggtccttt ggggtgcttc tctgggagat cttctctctg ggggcctccc cgtaccctgg
     8641 ggtgcagatc aatgaggagt tctgccagcg gctgagagac ggcacaagga tgagggcccc
     8701 ggagctggcc actcccgcca tacgccgcat catgctgaac tgctggtccg gagaccccaa
     8761 ggcgagacct gcattctcgg agctggtgga gatcctgggg gacctgctcc agggcagggg
     8821 ccttcaagag gaagaggagg tctgcatggc cccgcgcagc tctcagagct cagaagaggg
     8881 cagcttctcg caggtgtcca ccatggccct acacatcgcc caggctgacg ctgaggacag
     8941 cccgccaagc ctgcagcgcc acagcctggc cgccaggtat tacaactggg tgtcctttcc
     9001 cgggtgcctg gccagagggg ctgagacccg tggttcctcc aggatgaaga catttgagga
     9061 attccccatg accccaacga cctacaaagg ctctgtggac aaccagacag acagtgggat
     9121 ggtgctggcc tcggaggagt ttgagcagat agagagcagg catagacaag aaagcggctt
     9181 cagctgtaaa ggacctggcc agaatgtggc tgtgaccagg gcacaccctg actcccaagg
     9241 gaggcggcgg cggcctgagc ggggggcccg aggaggccag gtgttttaca acagcgagta
     9301 tggggagctg tcggagccaa gcgaggagga ccactgctcc ccgtctgccc gcgtgacttt
     9361 cttcacagac aacagctacg caaagggtca agacaattct gcagatatcc agcacagtgg
     9421 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     9481 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt aaacccgctg
     9541 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     9601 ttccttgacc ctggaaggtg ccactcccac
//
