LOCUS       sequence-407180-        8943 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human TIE receptor tyrosine kinase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8943)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 8943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-TIE-V5/HIS
FEATURES             Location/Qualifiers
     source          1..8943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        59..438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        439..642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     593..613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     687..706
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        687..705
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(1319..1330)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4265..4306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4316..4333
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4356..4373)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4362..4586
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4632..5060
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4719..4738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4929..4950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5069..5089)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5074..5403
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5254..5389
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5316..5335
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5470..6264
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5524..5543)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6134..6153
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6440..6561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6477..6496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6531..6550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6610..6626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6610..6626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6623..6645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6634..6650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6658..6664,6665..6682,6683..6688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6703..6724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6841..6858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7012..7600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7092..7111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7771..8562,8563..8631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8394..8413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8632..8736)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8811..8830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
       61 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
      121 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
      181 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      361 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
      661 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagctggc
      721 tagttaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tgcccttcac
      781 catggtctgg cgggtgcccc ctttcttgct ccccatcctc ttcttggctt ctcatgtggg
      841 cgcggcggtg gacctgacgc tgctggccaa cctgcggctc acggaccccc agcgcttctt
      901 cctgacttgc gtgtctgggg aggccggggc ggggaggggc tcggacgcct ggggcccgcc
      961 cctgctgctg gagaaggacg accgtatcgt gcgcaccccg cccgggccac ccctgcgcct
     1021 ggcgcgcaac ggttcgcacc aggtcacgct tcgcggcttc tccaagccct cggacctcgt
     1081 gggcgtcttc tcctgcgtgg gcggtgctgg ggcgcggcgc acgcgcgtca tctacgtgca
     1141 caacagccct ggagcccacc tgcttccaga caaggtcaca cacactgtga acaaaggtga
     1201 caccgctgta ctttctgcac gtgtgcacaa ggagaagcag acagacgtga tctggaagag
     1261 caacggatcc tacttctaca ccctggactg gcatgaagcc caggatgggc ggttcctgct
     1321 gcagctccca aatgtgcagc caccatcgag cggcatctac agtgccactt acctggaagc
     1381 cagccccctg ggcagcgcct tctttcggct catcgtgcgg ggttgtgggg ctgggcgctg
     1441 ggggccaggc tgtaccaagg agtgcccagg ttgcctacat ggaggtgtct gccacgacca
     1501 tgacggcgaa tgtgtatgcc cccctggctt cactggcacc cgctgtgaac aggcctgcag
     1561 agagggccgt tttgggcaga gctgccagga gcagtgccca ggcatatcag gctgccgggg
     1621 cctcaccttc tgcctcccag acccctatgg ctgctcttgt ggatctggct ggagaggaag
     1681 ccagtgccaa gaagcttgtg cccctggtca ttttggggct gattgccgac tccagtgcca
     1741 gtgtcagaat ggtggcactt gtgaccggtt cagtggttgt gtctgcccct ctgggtggca
     1801 tggagtgcac tgtgagaagt cagaccggat cccccagatc ctcaacatgg cctcagaact
     1861 ggagttcaac ttagagacga tgccccggat caactgtgca gctgcaggga accccttccc
     1921 cgtgcggggc agcatagagc tacgcaagcc agacggcact gtgctcctgt ccaccaaggc
     1981 cattgtggag ccagagaaga ccacagctga gttcgaggtg ccccgcttgg ttcttgcgga
     2041 cagtgggttc tgggagtgcc gtgtgtccac atctggcggc caagacagcc ggcgcttcaa
     2101 ggtcaatgtg aaagtgcccc ccgtgcccct ggctgcacct cggctcctga ccaagcagag
     2161 ccgccagctt gtggtctccc cgctggtctc gttctctggg gatggaccca tctccactgt
     2221 ccgcctgcac taccggcccc aggacagtac catggactgg tcgaccattg tggtggaccc
     2281 cagtgagaac gtgacgttaa tgaacctgag gccaaagaca ggatacagtg ttcgtgtgca
     2341 gctgagccgg ccaggggaag gaggagaggg ggcctggggg cctcccaccc tcatgaccac
     2401 agactgtcct gagcctttgt tgcagccgtg gttggagggc tggcatgtgg aaggcactga
     2461 ccggctgcga gtgagctggt ccttgccctt ggtgcccggg ccactggtgg gcgacggttt
     2521 cctgctgcgc ctgtgggacg ggacacgggg gcaggagcgg cgggagaacg tctcatcccc
     2581 ccaggcccgc actgccctcc tgacgggact cacgcctggc acccactacc agctggatgt
     2641 gcagctctac cactgcaccc tcctgggccc ggcctcgccc cctgcacacg tgcttctgcc
     2701 ccccagtggg cctccagccc cccgacacct ccacgcccag gccctctcag actccgagat
     2761 ccagctgaca tggaagcacc cggaggctct gcctgggcca atatccaagt acgttgtgga
     2821 ggtgcaggtg gctgggggtg caggagaccc actgtggata gacgtggaca ggcctgagga
     2881 gacaagcacc atcatccgtg gcctcaacgc cagcacgcgc tacctcttcc gcatgcgggc
     2941 cagcattcag gggctcgggg actggagcaa cacagtagaa gagtccaccc tgggcaacgg
     3001 gctgcaggct gagggcccag tccaagagag ccgggcagct gaagagggcc tggatcagca
     3061 gctgatcctg gcggtggtgg gctccgtgtc tgccacctgc ctcaccatcc tggccgccct
     3121 tttaaccctg gtgtgcatcc gcagaagctg cctgcatcgg agacgcacct tcacctacca
     3181 gtcaggctcg ggcgaggaga ccatcctgca gttcagctca gggaccttga cacttacccg
     3241 gcggccaaaa ctgcagcccg agcccctgag ctacccagtg ctagagtggg aggacatcac
     3301 ctttgaggac ctcatcgggg aggggaactt cggccaggtc atccgggcca tgatcaagaa
     3361 ggacgggctg aagatgaacg cagccatcaa aatgctgaaa gagtatgcct ctgaaaatga
     3421 ccatcgtgac tttgcgggag aactggaagt tctgtgcaaa ttggggcatc accccaacat
     3481 catcaacctc ctgggggcct gtaagaaccg aggttacttg tatatcgcta ttgaatatgc
     3541 cccctacggg aacctgctag attttctgcg gaaaagccgg gtcctagaga ctgacccagc
     3601 ttttgctcga gagcatggga cagcctctac ccttagctcc cggcagctgc tgcgtttcgc
     3661 cagtgatgcg gccaatggca tgcagtacct gagtgagaag cagttcatcc acagggacct
     3721 ggctgcccgg aatgtgctgg tcggagagaa cctagcctcc aagattgcag acttcggcct
     3781 ttctcgggga gaggaggttt atgtgaagaa gacgatgggg cgtctccctg tgcgctggat
     3841 ggccattgag tccctgaact acagtgtcta taccaccaag agtgatgtct ggtcctttgg
     3901 agtccttctt tgggagatag tgagccttgg aggtacaccc tactgtggca tgacctgtgc
     3961 cgagctctat gaaaagctgc cccagggcta ccgcatggag cagcctcgaa actgtgacga
     4021 tgaagtgtac gagctgatgc gtcagtgctg gcgggaccgt ccctatgagc gacccccctt
     4081 tgcccagatt gcgctacagc taggccgcat gctggaagcc aggaaggcct atgtgaacat
     4141 gtcgctgttt gagaacttca cttacgcggg cattgatgcc acagctgagg aggccgcaaa
     4201 gggcaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccgcggtt
     4261 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca
     4321 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4381 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4441 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4501 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4561 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4621 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4681 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4741 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4801 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4861 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4921 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4981 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5041 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5101 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5161 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5221 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5281 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5341 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5401 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5461 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5521 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5581 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5641 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5701 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5761 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5821 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5881 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5941 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     6001 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6061 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6121 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6181 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6241 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgcgaaatga ccgaccaagc
     6301 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6361 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6421 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     6481 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     6541 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     6601 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     6661 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     6721 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     6781 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     6841 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     6901 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     6961 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7021 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7081 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7141 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7201 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7261 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7321 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7381 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7441 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7501 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7561 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7621 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7681 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7741 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     7801 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     7861 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     7921 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     7981 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     8041 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     8101 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     8161 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     8221 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     8281 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     8341 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     8401 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     8461 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     8521 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     8581 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     8641 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     8701 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     8761 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     8821 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     8881 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     8941 aat
//
