LOCUS       sequence-407187-        8342 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human ROR2 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ROR2-V5/HIS
FEATURES             Location/Qualifiers
     source          1..8342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(95..114)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      908..1496
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1397..1416
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1650..1667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1784..1805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1820..1825,1826..1843,1844..1850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1858..1874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1863..1885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1882..1898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1882..1898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1947..2068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1958..1977)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2012..2031
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2244..3038)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2355..2374)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2965..2984
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3105..3434)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3119..3254
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3173..3192)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3419..3439
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3448..3876)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3558..3579)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3770..3789
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3922..4146
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4135..4152
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4175..4192)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(4202..4243)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(7201..7220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7202..7220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7265..7468)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7294..7314)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7469..7848
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8020..8039
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8114..8218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8219..8287,8288..8342,1..737)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
       61 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
      121 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
      181 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
      241 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
      301 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      361 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      421 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      481 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      541 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      601 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      661 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      721 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      781 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      841 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      901 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      961 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1021 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     1081 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1141 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1201 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1261 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1321 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1381 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1441 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1501 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     1561 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     1621 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     1681 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     1741 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     1801 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     1861 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc
     1921 tagaggtcga cggtatacag acatgataag atacattgat gagtttggac aaaccacaac
     1981 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     2041 aaccattata agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc
     2101 cgcgctggag gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat
     2161 agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca
     2221 tttcgcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     2281 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     2341 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     2401 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     2461 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     2521 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     2581 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     2641 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     2701 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     2761 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     2821 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac
     2881 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     2941 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     3001 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc
     3061 tcttgatcag atccgaaaat ggatatacaa gctcccggga gctttttgca aaagcctagg
     3121 cctccaaaaa agcctcctca ctacttctgg aatagctcag aggcagaggc ggcctcggcc
     3181 tctgcataaa taaaaaaaat tagtcagcca tggggcggag aatgggcgga actgggcgga
     3241 gttaggggcg ggatgggcgg agttaggggc gggactatgg ttgctgacta attgagatgc
     3301 atgctttgca tacttctgcc tgctggggag cctggggact ttccacacct ggttgctgac
     3361 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     3421 cctaactgac acacattcca cagaattaat tcgcgttaaa tttttgttaa atcagctcat
     3481 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3541 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3601 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3661 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     3721 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     3781 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     3841 cacccgccgc gcttaatgcg ccgctacagg gcgcgtgggg atacccccta gagccccagc
     3901 tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt
     3961 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
     4021 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
     4081 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
     4141 gcacagtcga ggctgatcag cgggtttaaa ctcaatggtg atggtgatga tgaccggtac
     4201 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
     4261 ctagactcga gcggccgcca ctgtgctgga tatctgcaga attgtcttga cccttagctt
     4321 ccagttggac ttgggcctcg tccacctgca gagtgtcaca gtcccccagc agctcagtct
     4381 ctgggacaga gccttcctcc tcctcctctg cttcctgcac ggtgctctgg gccccatctt
     4441 ctggggcgtt ctgtgtgtca tcagcgccct ctgagagcag ggctgccctg tctgccatgg
     4501 atgtgttgga gggggccgtg gtgacgtagc ctgtgctggt ggagccactg ccactgtggt
     4561 gtgagctggg cttggggacc atctgaggag gcacctgctg cggggccatc tgcattggga
     4621 tctgcaccgg gtagaagttg ggcaggtagg ccccataggc cggcaccggc tggtagccgt
     4681 tgacggggac gtagagctgc ggcgggggca ccatgggtct gatctggccc ttcatgggga
     4741 tgaactgggg ctgtgggaag ggcggggcct tctgcttggg ccccacgtag cgggcgttgc
     4801 tcacattgct cactgggctg gtgctcaggg agctggtctg cgtggtgttg ctggcccccg
     4861 aggtctgcgc cgagctgttg tagttggaaa ggttgcccca ggctcggagc cggctgtgga
     4921 tgtccttgaa gcggggccgc cggctgggga actcgttcca gcactcgatc atgagggcat
     4981 acacccaggc gggacagtca tcggggcaag gcagcacctg ccggttccgg atcatctcca
     5041 ccacatcctg gttggaatac ccgcagtagg gctgcaggcc gtagctgaag acctcccaca
     5101 ggaccacacc gtaggaccag atgtctgagt cgatggagaa cttgccgtac atgatggcct
     5161 ctggggccat ccagcggata ggcagcagcg agttccccag cagcttgtag taatcggcgg
     5221 catacacctc tcggaagagg cccaagtctg agatcttcac gttcagcttg tcgtacacta
     5281 gcacattgcg ggtggccagg tccttgtgaa ccacgtggtg gctggatagg tactccatcc
     5341 ccgccgcgat ctgtgccaca aggtgcacga agtcgggggg ctccagggcg gacttcaccg
     5401 tgcggtcatc atcggtgctg cccacgtccg agtgcggcga gcgcatgacc aggaattcgt
     5461 ggaggtcgcc gtgcgaacag tagctgaaga tcatgctcag gggctggtcc ttggtcacca
     5521 cgcccagcag gcagacgacg ttggggtgtt gcagccgtgc tcgcagcata gcctcatgcc
     5581 ggaactcctc ccgcaggggc ccctccgctt tgtccttcag cgttttgatg gccacagcct
     5641 gggtctgctc ccccggggca gggccgaaca ggtgaccttt gtagactttc ccaaaccggt
     5701 cctctcccag ctcctccatg aacctcaccg cagacaggct gatctctttg agtttggcct
     5761 gtttgtgctg gttaatgagg ggcatttcca tgtcttggct gggcgaggcc atcagctgtc
     5821 gccgctgcgg tgtggacgca gatgccttct gcttattccg gcacatgcaa accaagaaga
     5881 aaaggcaagc gatgaccagt ggaattgcga tgctggggac caagatgtac agaatcccca
     5941 tcttgctgct gtctcgggga ctacacgagg gtacgtcaca cagttccatg cgtacgtttt
     6001 tattctgcgt aaagcaccag gggccctcca tctggcctcc ggggttccgg cagtaggcgt
     6061 gcccccctcc aagctcaggg aagtctgtgc tggacaggtg gtggctgtgg gggtgctgca
     6121 gggcccacgg ctggcactgg tggcctgact tggtggtgct tgccgttcct ctgtaatcca
     6181 tgcctgagcc gttatagcac tgatggtagc ggcccagcct ctcggctggg atgccaatgc
     6241 gcatgcagtt ggcagcgtcg gggctctcag gcatgggcag cgcctcacac ttgggcagct
     6301 gaagccgcat gaggatgagc gggttggagc gggcgatggt gtactcctgg cggcacaggt
     6361 cgctctccag cacctcgcac tcgtcgcggc acagctcacg cggcttgggc gtccgggagc
     6421 gcgcgtcgca cagaggaaac acgaagtggc agaaggatgg gatggcgaac tgtgagcact
     6481 ggtccgacag gtgcgtagac gtgccgatca tggtgaaggc cgctgtgatt cggttttcaa
     6541 tctccccctg catctgaagc gagtccacat aaatggtccg gttgccaatg aagcgtgcac
     6601 aggcaattcc ccggtaaggc tggcagaacc catcctcgtg gtaatcatcc tgaaagttat
     6661 gatttgggct gtgcgttgga cccagccgca caaacaggac gccagtggcg gtaatggtct
     6721 tcatcccgtt ggtggccacg cactggtagt agccagtgtc tgtcgtgtcc aggtcctgga
     6781 ttcgcagtcg tgaaccatat tctgtcttcc ggatgatgat ccgccgcggc tcctgcacca
     6841 ccggggcatc attctttagc caccgcacgt tagggggtgg gtttcctgcc accttgcagt
     6901 gcagaattgc cgtctggcct tggacaatgg tgatattgtt tactggctcc agaaaattca
     6961 gaaagtaacc tttcagagtt ggaatcgggc cgtcctgccc atcaaggggt cctaaagggt
     7021 cgttcggatc cagaacctcc acttcacctg aagtccggga cactgagagc agaagcgcgg
     7081 cggccgccca gacggccggg atgcacagca gcggccgccg cgggagcgcc gagcctcggg
     7141 ccatggtgaa gggtactgga tccgagctcg gtaccaagct taactagcca gcttgggtct
     7201 ccctatagtg agtcgtatta atttcgataa gccagtaagc agtgggttct ctagttagcc
     7261 agagagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     7321 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     7381 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     7441 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     7501 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7561 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7621 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7681 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7741 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7801 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
     7861 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
     7921 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
     7981 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
     8041 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
     8101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8341 ag
//
