LOCUS       sequence-407246-        8666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aubergine expression for Schneider cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8666)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 8666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: F20-pAc5.1 EGFP Aubergine
FEATURES             Location/Qualifiers
     source          1..8666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        8..2517
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2537..2539,2540..2542,2543..3253)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2537..2539,2540..2542,2543..3253)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2582..2603)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2843..2862)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3190..3211
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(3423..3440)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5924..5965
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5975..5992
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6015..6032)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6065..6199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6089..6108)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6143..6162
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6800..7388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6880..6899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7559..8350,8351..8419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8182..8201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8420..8524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8592..8610
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaaaaatcat gaatggcatc aactctgaat caaatctttg cagatgcacc tacttctcat
       61 ttccactgtc acatcatttt tccagatctc gctgcctgtt atgtggccca caaaccaaga
      121 cacgttttat ggccattaaa gctggctgat cgtcgccaaa caccaaatac ataatgaata
      181 tgtacacatt cgagaaagaa gcgatcaaag aagcgtcttc gggcggagta ggagaatgcg
      241 gaggagaagg agaacgagct gatctagtat ctctccacaa tccaatgcca actgaccaac
      301 tggccatatt cggagcaatt tgaagccaat ttccatcgcc tggcgatcgc tccattcttg
      361 gctatatgtt tttcaccgtt acccggggcc attttcaaag actcgtcggc aagataagat
      421 tgtgtcactc gctgtctctc ttcatttgtc gaagaatgct gaggaatttc gcgatgacgt
      481 cggcgagtat tttgaagaat gagaataatt tgtatttata cgaaaatcag ttagtggaat
      541 tttctacaaa aacatgttat ctatagataa ttttgttgca aaatatgttg actatgacaa
      601 agattgtatg tatatacctt taatgtattc tcattttctt atgtatttat aatggcaatg
      661 atgatactga tgatatttta agatgatgcc agaccaaaag gcttgaattt ctgcgtcttt
      721 tgccgaacgc agtgcatgtg caattgttgt tttttggaat attcaatttt cggactgtcc
      781 gctttgattt cagtttcttg gcttattcaa aaagcaaagt aaagccaaaa aagcgagatg
      841 gcaataccaa atgcggcaaa acggtagtgg aaggaaaggg gtgcggggca gcggaaggaa
      901 gggtggggcg gggcgtggcg gggtctgtgg ctgggcgcga cgtcaccgac gttggagcca
      961 ctcctttgac catgtgtgcg tgtgtgtatt attcgtgtct cgccactcgc cggttgtttt
     1021 tttcttttta tgctgcgctc tctctagcgc catctcgctt acgcatgctc aacgcaccgc
     1081 atgttgccgt ttccttttat gcgtcatttt ggctcgaaat aggcaattat ttaaacaaag
     1141 attagtcaac gaaaacgcta aaataaataa gtctacaata tggttactta ttgccatgtg
     1201 tgtgcagcca acgatagcaa caaaagcaac aacacaggtg gctttccctc tttcactttt
     1261 tgtttgcaag ccgcgtgcga gcaagacggc acgaccggca aacgcaatta cgctgacaaa
     1321 gagcagacga agttttggcg aaaaacatca aggcgcctga tacgaatgca tttgcaataa
     1381 caattgcgat atttaatatt gtttatgaag ctgtttgact tcaaaacaca caaaaaaaaa
     1441 aataaaacaa attatttgaa agagaattag gaatcggacg cttatcgtta gggtaacaac
     1501 aagaaatgct tactgagtca cagcctctgg aaaactgccg caagccagag agagagagaa
     1561 aaagagggag agcagcttag accgcatgtg cttgtgtgtg aggcgtctct ctcttcgtct
     1621 ctgttgcgca aacgcataga ctgcactgaa aaaatcgatt acctattttt tatgaatgaa
     1681 tatttgcact attactattc aaaactatta agatagcaat cacattcaat agccaaatac
     1741 tataccacct gagcgatgca acgaaatgat caatttgagc aaaaatgctg catatttagg
     1801 acggcatcat tatagaaatg cttcttgctg tgtacttttc tctcgtctgg cagctgtttc
     1861 gccgttattg ttaaaaccgg cttaagttag gtgtgttttc tacgactagt gaatgcccta
     1921 ctagaagatg tgtgttgcac aaaatgtccc tggaataacc aatttgaagt gcagatagca
     1981 gtaaacgtaa gctaatatga atattattta actgtaatgt tttaatatcg ctggacatta
     2041 ctaataaacc cactataaac acatgtacat atgtatgttt tggcatacaa tgagtagttg
     2101 gggaaaaaat gtgtaaaagc accgtgacca tcacagcata aagataacca gctgaagtat
     2161 cgaatatgag taacccccaa attgaatcac atgccgcaac tgataggacc catggaagta
     2221 cactcttcat ggcgatatac aagacacaca caagcacgaa cacccagttg cggaggaaat
     2281 tctccgtaaa tgaaaaccca atcggcgaac aattcatacc catatatggt aaaagttttg
     2341 aacgcgactt gagagcggag agcattgcgg ctgataaggt tttagcgcta agcgggcttt
     2401 ataaaacggg ctgcgggacc agttttcata tcactaccgt ttgagttctt gtgctgtgtg
     2461 gatactcctc ccgacacaaa gccgctccat cagccagcag tcgtctaatc cagagacccc
     2521 ggatcggggt accatcatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2581 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2641 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2701 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2761 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2821 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2881 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     2941 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3001 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3061 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3121 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     3181 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3241 cgagctgtac aagccaagct tgaattctgc agatatgaat ttaccaccaa accctgtaat
     3301 tgctcgtgga cgtggtcgag gaagaaagcc caataatgta gaggccaatc gcggcttcgc
     3361 tccttcgctg ggtcagaagt cggacccttc ccactcggaa ggaaatcaag catccggagg
     3421 caatggtggt ggtggtgatg ctcaagtggg tccctcgata gagaaatcca gtttgagcgc
     3481 ggtgcaaatg cacaagtccg agggcgatcc gcgtggctcc gtgcgcggcc gtaggctaat
     3541 aacggatttg gtgtattcac gtcctccagg gatgaccagc aagaaaggag tggttggcac
     3601 tcatattacc gtgcaggcaa actattttaa ggtattaaag cgtccaaact ggaccatcta
     3661 ccagtaccgc gtcgatttta cgcctgatgt ggaggctaca cgactgcgac ggtctttctt
     3721 gtatgaacat aaagggatcc tgggcggcta catctttgac ggaaccaata tgttttgcat
     3781 caatcagttc aaagctgttc aagatagccc ctatgttttg gaactggtta cgaagagtcg
     3841 tgctggcgaa aacattgaaa ttaagatcaa ggctgttgga tctgtgcaat ctacggatgc
     3901 cgagcagttt caggtcctta atctcatact gcgcagggcc atggagggct tagacttgaa
     3961 gctggtctcg cgctactact acgatcctca agctaagatt aatttggaga atttccgcat
     4021 gcaattatgg cctggctatc agacttcgat tcgccagcac gaaaatgata tattactttg
     4081 ttccgagata tgccacaagg ttatgcgaac tgagaccttg tacaatattt tgtccgatgc
     4141 tattcgcgac agtgacgatt atcaaagtac atttaagcgt gcagtaatgg gtatggtaat
     4201 actaacggat tataataaca aaacctatcg cattgacgat gtcgactttc aatcgacgcc
     4261 attgtgcaaa tttaaaacca atgacggtga gatttcgtac gtggattact ataagaagcg
     4321 atacaacata atcattcgcg acttgaagca acctctggtc atgtctcgtc cgacagacaa
     4381 aaatattcgt ggtggaaatg accaggccat aatgatcatt cccgagctgg cacgggctac
     4441 gggaatgacg gacgctatgc gcgctgactt taggacgttg agggccatga gtgaacatac
     4501 caggctgaat ccagatcgtc gcatcgaacg ccttcgcatg ttcaacaagc gcttaaagtc
     4561 gtgtaaacag agtgtagaga cccttaagtc ctggaacatc gagctggact ccgccttggt
     4621 ggagattcca gcacgcgtgt tgccaccgga aaaaatatta ttcggcaacc aaaaaatatt
     4681 cgtatgcgac gctcgcgccg attggaccaa tgagtttcgc acctgttcga tgtttaaaaa
     4741 cgtgcatatc aataggtggt atgtgatcac accgagtcga aatctgcgcg agacccagga
     4801 atttgtgcag atgtgcatcc gcacagccag cagcatgaag atgaacatct gcaatccaat
     4861 atatgaggaa attccggatg accgcaacgg cacttactcc caagcgatcg acaacgccgc
     4921 agctaacgat ccacagatcg tgatggttgt catgagatct ccaaatgaag agaaatatag
     4981 ctgcattaaa aaacgcacgt gcgtggacag accggtgcca tcgcaggtgg tgacactaaa
     5041 agttatcgcg cctcgacagc aaaaaccaac tgggctgatg tcgatcgcca caaaggtggt
     5101 tattcaaatg aacgccaaat tgatgggagc tccctggcag gtagtgatcc ccctccacgg
     5161 tctgatgact gttggtttcg atgtctgcca ttcaccgaag aataagaaca agtcatatgg
     5221 ggctttcgtt gcaaccatgg accagaagga gtctttccgc tacttctcca ccgtaaacga
     5281 acacattaag ggccaagagt tgtccgagca gatgtcggtg aacatggcgt gcgccctgag
     5341 gtcgtatcag gagcaacacc gttctttgcc agagcgcatt ctcttctttc gcgacggtgt
     5401 tggcgatggt cagctctacc aggtggtaaa cagcgaggtg aacaccctaa aggacaggct
     5461 cgacgaaatt tacaaatcag ctggcaaaca ggagggctgt cgcatgacat ttataattgt
     5521 atccaagcgc attaattctc gctactttac tgggcatcgc aacccagttc cgggcactgt
     5581 agtcgatgac gttattacct tgccagagcg ctacgacttc ttcctagtgt cccaggctgt
     5641 tcggatagga actgtgtcgc ctaccagcta caatgttatt tctgacaaca tgggactaaa
     5701 cgccgataag ctgcagatgc tctcctataa gatgacccat atgtactaca attacagcgg
     5761 aaccatacga gtccccgctg tctgccatta cgcccacaaa ttggctttcc tcgtagccga
     5821 atccattaat cgtgcgcctt cagcaggact gcagaatcaa ttgtactttt tgtaaatcca
     5881 gcacagtggc ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     5941 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     6001 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     6061 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6121 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6181 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6241 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6301 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6361 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6421 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6481 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6541 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     6601 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     6661 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     6721 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     6781 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6841 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6901 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6961 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7021 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7081 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7141 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7201 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7261 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7321 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7381 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7441 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7501 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7561 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7621 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7681 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7741 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7801 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7861 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7921 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7981 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8041 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8101 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8161 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8221 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8281 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8341 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8401 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8461 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8521 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8581 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaatta attctatatt
     8641 ctaaaaacac aaatgatact tctaaa
//
