LOCUS       sequence-407286-        5468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Schneider cell imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: T8-pAc5.1 lambdaN HA EcoRV
FEATURES             Location/Qualifiers
     source          1..5468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(161..749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(241..260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(920..1711,1712..1780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1543..1562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1781..1885)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1953..1971
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2035..4544
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             4564..4629
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             4654..4680
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4753..4794
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4804..4821
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4844..4861)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4894..5028
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4918..4937)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4972..4991
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt
       61 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc
      121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      601 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
      841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
      901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
      961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     1021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     1081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     1141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     1201 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
     1261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     1321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     1381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     1441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     1501 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
     1561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     1621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     1681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     1741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     1801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     1861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     1921 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     1981 ttcaagaatt aattctatat tctaaaaaca caaatgatac ttctaaaaaa aaatcatgaa
     2041 tggcatcaac tctgaatcaa atctttgcag atgcacctac ttctcatttc cactgtcaca
     2101 tcatttttcc agatctcgct gcctgttatg tggcccacaa accaagacac gttttatggc
     2161 cattaaagct ggctgatcgt cgccaaacac caaatacata atgaatatgt acacattcga
     2221 gaaagaagcg atcaaagaag cgtcttcggg cggagtagga gaatgcggag gagaaggaga
     2281 acgagctgat ctagtatctc tccacaatcc aatgccaact gaccaactgg ccatattcgg
     2341 agcaatttga agccaatttc catcgcctgg cgatcgctcc attcttggct atatgttttt
     2401 caccgttacc cggggccatt ttcaaagact cgtcggcaag ataagattgt gtcactcgct
     2461 gtctctcttc atttgtcgaa gaatgctgag gaatttcgcg atgacgtcgg cgagtatttt
     2521 gaagaatgag aataatttgt atttatacga aaatcagtta gtggaatttt ctacaaaaac
     2581 atgttatcta tagataattt tgttgcaaaa tatgttgact atgacaaaga ttgtatgtat
     2641 atacctttaa tgtattctca ttttcttatg tatttataat ggcaatgatg atactgatga
     2701 tattttaaga tgatgccaga ccaaaaggct tgaatttctg cgtcttttgc cgaacgcagt
     2761 gcatgtgcaa ttgttgtttt ttggaatatt caattttcgg actgtccgct ttgatttcag
     2821 tttcttggct tattcaaaaa gcaaagtaaa gccaaaaaag cgagatggca ataccaaatg
     2881 cggcaaaacg gtagtggaag gaaaggggtg cggggcagcg gaaggaaggg tggggcgggg
     2941 cgtggcgggg tctgtggctg ggcgcgacgt caccgacgtt ggagccactc ctttgaccat
     3001 gtgtgcgtgt gtgtattatt cgtgtctcgc cactcgccgg ttgttttttt ctttttatgc
     3061 tgcgctctct ctagcgccat ctcgcttacg catgctcaac gcaccgcatg ttgccgtttc
     3121 cttttatgcg tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa
     3181 aacgctaaaa taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg
     3241 atagcaacaa aagcaacaac acaggtggct ttccctcttt cactttttgt ttgcaagccg
     3301 cgtgcgagca agacggcacg accggcaaac gcaattacgc tgacaaagag cagacgaagt
     3361 tttggcgaaa aacatcaagg cgcctgatac gaatgcattt gcaataacaa ttgcgatatt
     3421 taatattgtt tatgaagctg tttgacttca aaacacacaa aaaaaaaaat aaaacaaatt
     3481 atttgaaaga gaattaggaa tcggacgctt atcgttaggg taacaacaag aaatgcttac
     3541 tgagtcacag cctctggaaa actgccgcaa gccagagaga gagagaaaaa gagggagagc
     3601 agcttagacc gcatgtgctt gtgtgtgagg cgtctctctc ttcgtctctg ttgcgcaaac
     3661 gcatagactg cactgaaaaa atcgattacc tattttttat gaatgaatat ttgcactatt
     3721 actattcaaa actattaaga tagcaatcac attcaatagc caaatactat accacctgag
     3781 cgatgcaacg aaatgatcaa tttgagcaaa aatgctgcat atttaggacg gcatcattat
     3841 agaaatgctt cttgctgtgt acttttctct cgtctggcag ctgtttcgcc gttattgtta
     3901 aaaccggctt aagttaggtg tgttttctac gactagtgaa tgccctacta gaagatgtgt
     3961 gttgcacaaa atgtccctgg aataaccaat ttgaagtgca gatagcagta aacgtaagct
     4021 aatatgaata ttatttaact gtaatgtttt aatatcgctg gacattacta ataaacccac
     4081 tataaacaca tgtacatatg tatgttttgg catacaatga gtagttgggg aaaaaatgtg
     4141 taaaagcacc gtgaccatca cagcataaag ataaccagct gaagtatcga atatgagtaa
     4201 cccccaaatt gaatcacatg ccgcaactga taggacccat ggaagtacac tcttcatggc
     4261 gatatacaag acacacacaa gcacgaacac ccagttgcgg aggaaattct ccgtaaatga
     4321 aaacccaatc ggcgaacaat tcatacccat atatggtaaa agttttgaac gcgacttgag
     4381 agcggagagc attgcggctg ataaggtttt agcgctaagc gggctttata aaacgggctg
     4441 cgggaccagt tttcatatca ctaccgtttg agttcttgtg ctgtgtggat actcctcccg
     4501 acacaaagcc gctccatcag ccagcagtcg tctaatccag agaccccgga tcggggtacc
     4561 aacatggacg cacaaacacg acgacgtgag cgtcgcgctg agaaacaagc tcaatggaaa
     4621 gctgcaaacg gtggatctcc tccacatatg gcttacccat acgatgttcc agattacgct
     4681 cctccaagct tgaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc
     4741 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
     4801 ggtcatcatc accatcacca ttgagtttaa acccgctgat cagcctcgac tgtgccttct
     4861 aaggcctgag ctcgctgatc agcctcgatc gaggatccag acatgataag atacattgat
     4921 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     4981 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     5041 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
     5101 aacctctaca aatgtggtat ggctgattat gatcagtcga ccgatgccct tgagagcctt
     5161 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     5221 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     5281 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     5341 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     5401 gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     5461 gacgcgag
//
