LOCUS       sequence-407290-        9868 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific dsRed with CctraF, eGFP in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795H
FEATURES             Location/Qualifiers
     source          1..9868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    4643..5028
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        5035..5517
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        5521..6112
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             join(6125..6127,6128..6841)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(6125..6127,6128..6838)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6167..6188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6428..6447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6775..6796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6951..7072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6988..7007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7042..7061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7100..7169
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   7639..7673
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7680..7698)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7719..7735)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7719..7735)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7732..7754)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7743..7759
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7767..7773,7774..7791,7792..7797))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7812..7833
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7950..7967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8201..8220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8880..9671,9672..9740))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9503..9522
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9741..9845)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca cctatagata ccatagatgt
     2761 atggattagt atcatataca tacaaaggct atttttggga catattaata ttaacaattt
     2821 ccgtgatagt tttcaccatt tttgttgaat gttacgttga aaatttaaat ttgttttaaa
     2881 ttaattttac cagtcatgtg ttcttaaaag tttttatgat tgaaacggca taaagtggtt
     2941 caaaaattta tcaagaaagg ctttcctttt ttaaatctta tctttttctc ttaaaaatca
     3001 ctagtcaatt cattattaat ttgttaactt gaatttggaa tgtctattta ctttcagata
     3061 aattaaagca agaaacttaa tattcgaaaa aaattgattc taaatggaat ttcacttgat
     3121 cttcatgtat gcatatcaat ttttatttac attgtataat aagtttcgag ttgattgttg
     3181 taatccacag gtgtcccaga gaattaaatt ccaaattacc caagtttatt gaatgttgat
     3241 tgtagtttca gttgctttgt tgctgcaaca atggcttgtt gattgtagat attttccctt
     3301 tccttggttt acttattaca tagactgaaa aagaggttta cttttttgat acttatgaaa
     3361 aatttctatt agtgattact aaccaatcgc tatatgttta ctagaaaaca aataaactct
     3421 ttacattaac attcaataat gtttgctctg taaccgacaa ttgaaggcgt tacagcaaca
     3481 gtaatataac tagcttctta accctcatct attaacccca tcgtttaaaa cactatgtta
     3541 aatggtctaa caaatctaga tactaataga tgtcttatta cttagcagcc acagctgcaa
     3601 catccaagac aatttttgaa acttcttatt gagctcttgg cagcagaaat gttggtattt
     3661 ttcacagctt tctgaaagac cggcaccttc ctccggttcc cgtttctgaa ttcaagagga
     3721 tttccgaccc ccaattaatc ccgaaacaaa taaggtatat tcaaaatgat ggaaaagtca
     3781 tggctgctga ccttattttt attcctattg atagaatatt attccccttt taaatacact
     3841 gtactaagag gtccggctat aattttactc acttgtcgat tatcccatag aatgttgatt
     3901 gtagttggtt gcttttccag gtgagagttg atcaagtcac aaaagttagc gtgtgttgat
     3961 tgtagatttg aaggtaaaat aatttttgca cccattcatc gggtaaaacg ttctccatag
     4021 aatacatttc catcgataat tgataactta tgaatttcaa agaaaaaaat atgcttttaa
     4081 aattaccatc accagagaca ggttgcggcg gcggttggat ggcgtgggcg cgttggcgtt
     4141 gttggaccgg ctcatgttgt gtcgctgtaa cagatgctgt tcaactgtgt ttaccagatc
     4201 gttgcgggct gtatttatag gcgcgataag cgggacgggc gcctcgtgtc cggtcacgcg
     4261 catgagataa cgcgcggctg atatggaggc gcgtcctgtt ccgataagga gttgcgtccg
     4321 gctgcggtta gcaacacagg aagctggcgt cctgtcacga taagacaaca ctcgtccggt
     4381 ccgataatgt gattcgtacg tgacaggacg cgacccgata aggccggcct acgtgactgc
     4441 cgacacgtac ttttttgcac tgcaaaaagg ttcaatgtgt ggtagtgtat ttggagcgta
     4501 tacaacggtg tagactattt atgtaaaata gtctacgaaa cgtagagttt gtactatgta
     4561 tgggcccgcg tgcaaaagcg tgtttttttg cagtgcaaaa aagttggtgg tggggaggcc
     4621 accgagtatg ggcgcggtac ctcacgtaat aagtgtgcgt tgaatttatt cgcaaaaaca
     4681 ttgcatattt tcggcaaagt aaaattttgt tgcatacctt atcaaaaaat aagtgctgca
     4741 tactttttag agaaaccaaa taatttttta ttgcataccc gtttttaata aaatacattg
     4801 cataccctct tttaataaaa aatattgcat actttgacga aacaaatttt cgttgcatac
     4861 ccaataaaag attattatat tgcatacccg tttttaataa aatacattgc ataccctctt
     4921 ttaataaaaa atattgcata cgttgacgaa acaaattttc gttgcatacc caataaaaga
     4981 ttattatatt gcataccttt tcttgccata ccatttagcc gatcaattct cgagcgcgta
     5041 aaacacaatc aagtatgagt cataagctga tgtcatgttt tgcacacggc tcataaccga
     5101 actggcttta cgagtagaat tctacttgta acgcacgatc agtggatgat gtcatttgtt
     5161 tttcaaatcg agatgatgtc atgttttgca cacggctcat aaactcgctt tacgagtaga
     5221 attctacgtg taacgcacga tcgattgatg agtcatttgt tttgcaatat gatatcatac
     5281 aatatgactc atttgttttt caaaaccgaa cttgatttac gggtagaatt ctacttgtaa
     5341 agcacaatca aaaagatgat gtcatttgtt tttcaaaact gaactcgctt tacgagtaga
     5401 attctacgtg taaaacacaa tcaagaaatg atgtcatttg ttataaaaat aaaagctgat
     5461 gtcatgtttt gcacatggct cataactaaa ctcgctttac gggtagaatt ctacgcgcgt
     5521 cgatgtcttt gtgatgcgcg cgacattttt gtaggttatt gataaaatga acggatacgt
     5581 tgcccgacat tatcattaaa tccttggcgt agaatttgtc gggtccattg tccgtgtgcg
     5641 ctagcatgcc cgtaacggac ctcgtacttt tggcttcaaa ggttttgcgc acagacaaaa
     5701 tgtgccacac ttgcagctct gcatgtgtgc gcgttaccac aaatcccaac ggcgcagtgt
     5761 acttgttgta tgcaaataaa tctcgataaa ggcgcggcgc gcgaatgcag ctgatcacgt
     5821 acgctcctcg tgttccgttc aaggacggtg ttatcgacct cagattaatg tttatcggcc
     5881 gactgttttc gtatccgctc accaaacgcg tttttgcatt aacattgtat gtcggcggat
     5941 gttctatatc taatttgaat aaataaacga taaccgcgtt ggttttagag ggcataataa
     6001 aagaaatatt gttatcgtgt tcgccattag ggcagtataa attgacgttc atgttggata
     6061 ttgtttcagt tgcaagttga cactggcggc gacaagatcg tgaacaacca agtgacaata
     6121 caaaatgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     6181 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     6241 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     6301 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     6361 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     6421 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     6481 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     6541 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     6601 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     6661 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     6721 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     6781 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     6841 actagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     6901 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     6961 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7021 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taaagcttgt
     7081 cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct
     7141 ccccgggcgc gtactccacc tcacccatct ggtccatcat gatgaacggg tcgaggtggc
     7201 ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg
     7261 gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg
     7321 gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc gacagatatc tataacaaga
     7381 aaatatatat ataataagtt atcacgtaag tagaacatga aataacaata taattatcgt
     7441 atgagttaaa tcttaaaagt cacgtaaaag ataatcatgc gtcattttga ctcacgcggt
     7501 cgttatagtt caaaatcagt gacacttacc gcattgacaa gcacgcctca cgggagctcc
     7561 aagcggcgac tgagatgtcc taaatgcaca gcgacggatt cgcgctattt agaaagagag
     7621 agcaatattt caagaatgca tgcgtcaatt ttacgcagac tatctttcta gggttaatcc
     7681 ctttagtgag ggttaatttc gagcttggcg taatcatggt catagctgtt tcctgtgtga
     7741 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7801 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7861 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7921 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8521 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     8581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9181 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9241 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9301 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9361 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9421 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9481 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9541 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9601 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9661 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9721 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9781 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9841 ccgcgcacat ttccccgaaa agtgccac
//
