LOCUS       sequence-407291-        8771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific dsRed with DmtraF, eGFP in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795I
FEATURES             Location/Qualifiers
     source          1..8771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="VRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    3546..3931
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        3938..4420
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        4424..5015
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             join(5028..5030,5031..5744)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(5028..5030,5031..5741)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5070..5091)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5331..5350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5678..5699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5854..5975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5891..5910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5945..5964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6003..6072
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   6542..6576
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6583..6601)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6622..6638)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6622..6638)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6635..6657)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6646..6662
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6670..6676,6677..6694,6695..6700))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6715..6736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6853..6870)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7024..7612)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7104..7123)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7783..8574,8575..8643))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8406..8425
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8644..8748)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca cctatgtgaa aagagtgtgc
     2761 ggttagtcaa tgcatctaac gcatagtgtt tggcgcatcg tcggagacgg aatccacgta
     2821 atgcgcagtt gagagtccta ttgtcctagc ttctctttct ctgtctggtc gtccagcgtt
     2881 cattctcatc catttcacat ttcacacaat atgacactag aaaaaaaaca acaaaaagat
     2941 ggcactggat cagaatctga tcagatcggt tatactatat agtgggtacc atcaccagag
     3001 acaggttgcg gcggcggttg gatggcgtgg gcgcgttggc gttgttggac cggctcatgt
     3061 tgtgtcgctg taacagatgc tgttcaactg tgtttaccag atcgttgcgg gctgtattta
     3121 taggcgcgat aagcgggacg ggcgcctcgt gtccggtcac gcgcatgaga taacgcgcgg
     3181 ctgatatgga ggcgcgtcct gttccgataa ggagttgcgt ccggctgcgg ttagcaacac
     3241 aggaagctgg cgtcctgtca cgataagaca acactcgtcc ggtccgataa tgtgattcgt
     3301 acgtgacagg acgcgacccg ataaggccgg cctacgtgac tgccgacacg tacttttttg
     3361 cactgcaaaa aggttcaatg tgtggtagtg tatttggagc gtatacaacg gtgtagacta
     3421 tttatgtaaa atagtctacg aaacgtagag tttgtactat gtatgggccc gcgtgcaaaa
     3481 gcgtgttttt ttgcagtgca aaaaagttgg tggtggggag gccaccgagt atgggcgcgg
     3541 tacctcacgt aataagtgtg cgttgaattt attcgcaaaa acattgcata ttttcggcaa
     3601 agtaaaattt tgttgcatac cttatcaaaa aataagtgct gcatactttt tagagaaacc
     3661 aaataatttt ttattgcata cccgttttta ataaaataca ttgcataccc tcttttaata
     3721 aaaaatattg catactttga cgaaacaaat tttcgttgca tacccaataa aagattatta
     3781 tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc
     3841 atacgttgac gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc
     3901 ttttcttgcc ataccattta gccgatcaat tctcgagcgc gtaaaacaca atcaagtatg
     3961 agtcataagc tgatgtcatg ttttgcacac ggctcataac cgaactggct ttacgagtag
     4021 aattctactt gtaacgcacg atcagtggat gatgtcattt gtttttcaaa tcgagatgat
     4081 gtcatgtttt gcacacggct cataaactcg ctttacgagt agaattctac gtgtaacgca
     4141 cgatcgattg atgagtcatt tgttttgcaa tatgatatca tacaatatga ctcatttgtt
     4201 tttcaaaacc gaacttgatt tacgggtaga attctacttg taaagcacaa tcaaaaagat
     4261 gatgtcattt gtttttcaaa actgaactcg ctttacgagt agaattctac gtgtaaaaca
     4321 caatcaagaa atgatgtcat ttgttataaa aataaaagct gatgtcatgt tttgcacatg
     4381 gctcataact aaactcgctt tacgggtaga attctacgcg cgtcgatgtc tttgtgatgc
     4441 gcgcgacatt tttgtaggtt attgataaaa tgaacggata cgttgcccga cattatcatt
     4501 aaatccttgg cgtagaattt gtcgggtcca ttgtccgtgt gcgctagcat gcccgtaacg
     4561 gacctcgtac ttttggcttc aaaggttttg cgcacagaca aaatgtgcca cacttgcagc
     4621 tctgcatgtg tgcgcgttac cacaaatccc aacggcgcag tgtacttgtt gtatgcaaat
     4681 aaatctcgat aaaggcgcgg cgcgcgaatg cagctgatca cgtacgctcc tcgtgttccg
     4741 ttcaaggacg gtgttatcga cctcagatta atgtttatcg gccgactgtt ttcgtatccg
     4801 ctcaccaaac gcgtttttgc attaacattg tatgtcggcg gatgttctat atctaatttg
     4861 aataaataaa cgataaccgc gttggtttta gagggcataa taaaagaaat attgttatcg
     4921 tgttcgccat tagggcagta taaattgacg ttcatgttgg atattgtttc agttgcaagt
     4981 tgacactggc ggcgacaaga tcgtgaacaa ccaagtgaca atacaaaatg agcaagggcg
     5041 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
     5101 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
     5161 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
     5221 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
     5281 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
     5341 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
     5401 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
     5461 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
     5521 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
     5581 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
     5641 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
     5701 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaactagat cataatcagc
     5761 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5821 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5941 agttgtggtt tgtccaaact catcaatgta tcttaaagct tgtcgacgat gtaggtcacg
     6001 gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg gctccccggg cgcgtactcc
     6061 acctcaccca tctggtccat catgatgaac gggtcgaggt ggcggtagtt gatcccggcg
     6121 aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc tgggcgcggt ggtcacggtg
     6181 agcacgggac gtgcgacggc gtcggcgggt gcggatacgc ggggcagcgt cagcgggttc
     6241 tcgacggtca cggcgggcat gtcgacagat atctataaca agaaaatata tatataataa
     6301 gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa
     6361 agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc
     6421 agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg
     6481 tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat
     6541 gcatgcgtca attttacgca gactatcttt ctagggttaa tccctttagt gagggttaat
     6601 ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6661 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6721 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6781 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6841 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6901 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6961 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7021 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7081 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7141 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7201 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7261 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7321 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7381 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7441 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7501 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7561 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7621 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7681 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7741 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7801 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7861 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     7921 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     7981 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8041 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8101 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8161 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8221 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8281 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8341 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8401 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8461 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8521 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8581 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8641 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8701 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8761 aaaagtgcca c
//
