LOCUS       sequence-407298-        8087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of MPL-shRNA3; gene knock down of human MPL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8087)
  AUTHORS   Araki M, Yang Y, Masubuchi N, Hironaka Y, Takei H, Morishita S, 
            Mizukami Y, Kan S, Shirane S, Edahiro Y, Sunami Y, Ohsaka A, Komatsu
            N
  TITLE     Activation of the thrombopoietin receptor by mutant calreticulin in 
            CALR-mutant myeloproliferative neoplasms.
  JOURNAL   Blood. 2016 Jan 27. pii: blood-2015-09-671172.
  PUBMED    26817954
REFERENCE   2  (bases 1 to 8087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Blood. 2016
            Jan 27. pii: blood-2015-09-671172."
COMMENT     Sequence Label: pLKO.3R/MPL-shRNA3
FEATURES             Location/Qualifiers
     source          1..8087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(59..82)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(257..276)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     474..494
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     526..544
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      671..680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(689..1199)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(752..770)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(1491..1739)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(1549..1568)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(1719..1739)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(2256..2300)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2485..2718
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3211..3336
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3383..3563
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3564..3790)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(3818..3838)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3818..3836)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3857..3873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3857..3873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3870..3892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3881..3897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3905..3911,3912..3929,3930..3935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3950..3971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4088..4105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4259..4847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4339..4358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5018..5809,5810..5878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5641..5660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5879..5983)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6009..6464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6146..6167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6358..6377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6591..6613
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6605..6622
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6606..6622
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6632..6651
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6632..6650
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      6671..6806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6846..6967
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6857..6876)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6911..6930
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7039..7272
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     7457..7473
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7458..7474
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7477..8065
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7583..7594
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7992..8012
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(8084..8087,1..674))
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
ORIGIN
        1 aggaacaggt ggtggcggcc ctcggcgcgc tcgtactgct ccacgatggt gtagtcctcg
       61 ttgtgggagg tgatgtccag cttggagtcc acgtagtagt agccgggcag ctgcacgggc
      121 ttcttggcca tgtagatgga cttgaactcc accaggtagt ggccgccgtc cttcagcttc
      181 agggccttgt ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg ctcggtggag
      241 gcctcccagc ccatagtctt cttctgcatt acggggccgt cggaggggaa gttcacgccg
      301 atgaacttca ccttgtagat gaaggagccg tcctgcaggg aggagtcctg ggtcacggtc
      361 accacgccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc ggggaaggac
      421 agcttcttgt agtcggggat gtcggcgggg tgcttcacgt acaccttgga gccgtactgg
      481 aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt caccttcagc
      541 ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat ctcgaactcg
      601 tggccgttca cggagccctc catgcgcacc ttgaagcgca tgaactcctt gatgacgtcc
      661 tcggaggagg ccatggtggc ggatccccct ggggagagag gtcggtgatt cggtcaacga
      721 gggagccgac tgccgacgtg cgctccggag gcttgcagaa tgcggaacac cgcgcgggca
      781 ggaacagggc ccacactacc gccccacacc ccgcctcccg caccgcccct tcccggccgc
      841 tgctctcggc gcgccctgct gagcagccgc tattggccac agcccatcgc ggtcggcgcg
      901 ctgccattgc tccctggcgc tgtccgtctg cgagggtact agtgagacgt gcggcttccg
      961 tttgtcacgt ccggcacgcc gcgaaccgca aggaaccttc ccgacttagg ggcggagcag
     1021 gaagcgtcgc cggggggccc acaagggtag cggcgaagat ccgggtgacg ctgcgaacgg
     1081 acgtgaagaa tgtgcgagac ccagggtcgg cgccgctgcg tttcccggaa ccacgcccag
     1141 agcagccgcg tccctgcgca aacccagggc tgccttggaa aaggcgcaac cccaaccccc
     1201 tcgagccgcg gccaaagtgg atctctgctg tccctgtaat aaacccgaaa attttgaatt
     1261 tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt
     1321 ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg gatgaatact
     1381 gccatttgtc tcgaggtcga aaactggttt aattaagtag ctcgaggtgg aattcaaaaa
     1441 cctacctacc actaagctat tctcgagaat agcttagtgg taggtaggcc ggtgtttcgt
     1501 cctttccaca agatatataa agccaagaaa tcgaaatact ttcaagttac ggtaagcata
     1561 tgatagtcca ttttaaaaca taattttaaa actgcaaact acccaagaaa ttattacttt
     1621 ctacgtcacg tattttgtac taatatcttt gtgtttacag tcaaattaat tctaattatc
     1681 tctctaacag ccttgtatcg tatatgcaaa tatgaaggaa tcatgggaaa taggccctct
     1741 tcctgcccga ccttgtcgac aggactcgac tgacgcgtgt acccccttac ttgaggataa
     1801 attatgtcta atattcaaac tggcgccgag cgtatgccgc atgacctttc ccatcttggc
     1861 ttccttgctg gtcagattgg tcgtcttatt accatttcaa ctactccggt tatcgctggc
     1921 gactccttcg agatggacgc cgttggcgct ctccgtcttt ctccattgcg tcgtggtcga
     1981 ggctagtctc gtgatcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg
     2041 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     2101 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     2161 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     2221 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     2281 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     2341 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     2401 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     2461 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     2521 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     2581 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     2641 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     2701 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     2761 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     2821 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     2881 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     2941 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     3001 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     3061 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     3121 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     3181 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     3241 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     3301 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     3361 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     3421 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     3481 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     3541 atctggtcta accagagaga cccgtttatg tatcgagcta ggcacttaaa tacaatatct
     3601 ctgcaatgcg gcaattcagt ggttcgtcca atccatgtca gacccgtctg ttgccttcct
     3661 aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct
     3721 ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa gagtattgca
     3781 taagactaca ttaagcttgc agctccagct tttgttccct ttagtgaggg ttaattgcgc
     3841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     3901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     3961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     4021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     4081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     4141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     4201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcctttt
     4261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     4321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     4381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     4441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     4501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     4561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     4621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     4681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     4741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     4801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     4861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     4921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     4981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     5041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     5101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     5161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     5221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     5281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     5341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     5401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     5461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     5521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     5581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     5641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     5701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     5761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     5821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     5881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     5941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     6001 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6061 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     6361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     6421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     6481 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     6541 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     6601 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
     6661 tacgtcccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     6721 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     6781 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggatagc tagagccaga
     6841 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     6901 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     6961 acaagttcct ctcactctct gatattcatt tctttgcaag ttataaatac tgaataataa
     7021 gatgacatga actactactg ctagagattt tccacactga ctaaaagggt ctgagggatc
     7081 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
     7141 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
     7201 ggtctaacca gagagaccca gtacaagcaa aaagcagatc ttgtcttcgt tgggagtgaa
     7261 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     7321 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     7381 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     7441 catgtttttc taggtctcga ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga
     7501 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     7561 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     7621 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     7681 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     7741 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     7801 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     7861 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     7921 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     7981 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     8041 aaattttgta atccagaggt tgattatcga taagcttgat atcctac
//
