LOCUS       sequence-407303-        9828 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter of the interaction between SARS-CoV-2 spike and
            human ACE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9828)
  AUTHORS   Yang J, Xiao Y, Lidsky PV, Wu CT, Bonser LR, Peng S, Garcia-Knight 
            MA, Tassetto M, Chung CI, Li X, Nakayama T, Lee IT, Nayak JV, Ghias 
            K, Hargett KL, Shoichet BK, Erle DJ, Jackson PK, Andino R, Shu X
  TITLE     Fluorogenic reporter enables identification of compounds that 
            inhibit SARS-CoV-2.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):121-134. doi: 
            10.1038/s41564-022-01288-5. Epub 2023 Jan 5.
  PUBMED    36604514
REFERENCE   2  (bases 1 to 9828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01288-5"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "121-134"
COMMENT     Sequence Label: pCDNA3.1-spikeRBD::∆ACE2 SURF
FEATURES             Location/Qualifiers
     source          1..9828
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1634..2341
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             2513..2566
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2825..3532
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5419..5436)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5425..5649
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5695..6123
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5782..5801)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5992..6013
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6132..6152)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6137..6466
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6317..6452
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6379..6398
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6533..7327
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6587..6606)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7197..7216
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7501..7622
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7538..7557)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7592..7611
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7671..7687)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7671..7687)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7684..7706)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7695..7711
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7719..7725,7726..7743,7744..7749))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7764..7785
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7902..7919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8073..8661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8153..8172)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8832..9623,9624..9692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9455..9474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9693..9797)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 tgaattcgcc accatgaggg tacaacctac ggaatctatc gtacgcttcc cgaatatcac
      961 taacttgtgt ccgttcggcg aggtttttaa tgcgaccagg tttgcttccg tgtacgcctg
     1021 gaacaggaaa cggatctcca attgtgtcgc cgattactcc gtcttgtata attcagcatc
     1081 tttcagcacg tttaaatgtt acggagtttc ccccacaaaa ttgaatgacc tttgctttac
     1141 gaacgtctac gcggattcat ttgtaatccg gggggacgaa gttaggcaaa ttgcgccagg
     1201 gcagactggc aagatagctg actataatta taaattgccg gatgacttta cgggctgtgt
     1261 gattgcttgg aactcaaata atctggactc aaaggtaggg ggaaattata actaccttta
     1321 caggctgttc cggaagagta atctgaagcc attcgaaaga gatataagta cagagatcta
     1381 ccaagctgga agcaccccct gcaatggtgt tgaaggattc aattgttatt tcccattgca
     1441 atcctatggt tttcaaccga cgaatggggt gggataccaa ccatatcgag ttgtggttct
     1501 cagtttcgag ttgcttcatg ctcctgcgac agtatgtgga ccaaaaaaat ctactaatct
     1561 ggtgaagaat aaatgcgtca attttggatc cctcgagtcc ggactcagat ctggcagcgg
     1621 tggaggcgat atcatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1681 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1741 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1801 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1861 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1921 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1981 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     2041 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     2101 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     2161 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     2221 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     2281 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2341 gggatccggt gttaaaagcg tcgttaacct ggtgggagag aaattagtat acgtccaaaa
     2401 gtgggacggc aaggagacta cgcacgttag agagattaaa gacggcaagc tggtggtaac
     2461 actaacaatg ggcgacgtcg ttgcagtgcg ctcatatcgc agggcgacgg aggagggcag
     2521 aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct ggcccagtgc tccagaaatt
     2581 cgtaggaact tggaagatag cagattcaca taatttcggc gaatatctca gagccatagg
     2641 agcaccaaag gaattatcag atggcggtga tgccacaact cccactctgt atatcagcca
     2701 gaaagatggc gacaaaatga cggtgaaaat agagaacggc ccaccgacct tcctggacac
     2761 tcaggtgaag tttaaactgg gtgaggagtt tgacgagttt ccttctgacg ggcgtgggcg
     2821 cgccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     2881 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     2941 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     3001 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3061 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3121 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3181 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3241 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3301 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3361 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3421 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     3481 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca aggggcgcgc
     3541 cgctcagtcc accattgagg aacaggccaa gacatttttg gacaagttta accacgaagc
     3601 cgaagacctg ttctatcaaa gttcacttgc ttcttggaat tataacacca atattactga
     3661 agagaatgtc caaaacatga ataatgctgg ggacaaatgg tctgcctttt taaaggaaca
     3721 gtccacactt gcccaaatgt atccactaca agaaattcag aatctcacag tcaagcttca
     3781 gctgcaggct cttcagcaaa atgggtcttc agtgctctca gaagacaaga gcaaacggtt
     3841 gaacacaatt ctaaatacaa tgagcaccat ctacagtact ggaaaagttt gtaacccaga
     3901 taatccacaa gaatgcttat tacttgaacc aggtttgaat gaaataatgg caaacagttt
     3961 agactacaat gagaggctct gggcttggga aagctggaga tctgaggtcg gcaagcagct
     4021 gaggccatta tatgaagagt atgtggtctt gaaaaatgag atggcaagag caaatcatta
     4081 tgaggactat ggggattatt ggagaggaga ctatgaagta aatggggtag atggctatga
     4141 ctacagccgc ggccagttga ttgaagatgt ggaacatacc tttgaagaga ttaaaccatt
     4201 atatgaacat cttcatgcct atgtgagggc aaagttgatg aatgcctatc cttcctatat
     4261 cagtccaatt ggatgcctcc ctgctcattt gcttggtgat atgtggggta gattttggac
     4321 aaatctgtac tctttgacag ttccctttgg acagaaacca aacatagatg ttactgatgc
     4381 aatggtggac caggcctggg atgcacagag aatattcaag gaggccgaga agttctttgt
     4441 atctgttggt cttcctaata tgactcaagg attctgggaa aattccatgc taacggaccc
     4501 aggaaatgtt cagaaagcag tctgccatcc cacagcttgg gacctgggga agggcgactt
     4561 caggatcctt atgtgcacaa aggtgacaat ggacgacttc ctgacagctc atcatgagat
     4621 ggggcatatc cagtatgata tggcatatgc tgcacaacct tttctgctaa gaaatggagc
     4681 taatgaagga ttccatgaag ctgttgggga aatcatgtca ctttctgcag ccacacctaa
     4741 gcatttaaaa tccattggtc ttctgtcacc cgattttcaa gaagacaatg aaacagaaat
     4801 aaacttcctg ctcaaacaag cactcacgat tgttgggact ctgccattta cttacatgtt
     4861 agagaagtgg aggtggatgg tctttaaagg ggaaattccc aaagaccagt ggatgaaaaa
     4921 gtggtgggag atgaagcgag agatagttgg ggtggtggaa cctgtgcccc atgatgaaac
     4981 atactgtgac cccgcatctc tgttccatgt ttctaatgat tactcattca ttcgatatta
     5041 cacaaggacc ctttaccaat tccagtttca agaagcactt tgtcaagcag ctaaacatga
     5101 aggccctctg cacaaatgtg acatctcaaa ctctacagaa gctggacaga aactgttcaa
     5161 tatgctgagg cttggaaaat cagaaccctg gaccctagca ttggaaaatg ttgtaggagc
     5221 aaagaacatg aatgtaaggc cactgctcaa ctactttgag cccttattta cctggctgaa
     5281 agaccagaac aagaattctt ttgtgggatg gagtaccgac tggagtccat atgcagacta
     5341 agcggccgca taatagtgac tcttaatcgc gatccggata ttcgatttct agagggcccg
     5401 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5461 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5521 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     5581 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     5641 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     5701 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5761 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5821 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5881 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5941 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6001 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6061 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6121 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6181 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6241 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6301 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6361 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6421 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6481 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga
     6541 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6601 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6661 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga
     6721 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6781 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6841 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6901 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6961 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     7021 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga
     7081 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     7141 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     7201 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     7261 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     7321 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     7381 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     7441 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc
     7501 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     7561 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     7621 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg
     7681 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7741 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7801 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7861 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7921 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7981 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     8041 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     8101 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     8161 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     8221 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     8281 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     8341 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     8401 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     8461 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     8521 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8581 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8641 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8701 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8761 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8821 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8881 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8941 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     9001 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9061 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9121 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     9181 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9241 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9301 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9361 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9421 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9481 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9541 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9601 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9661 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9721 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9781 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
