LOCUS       sequence-407333-       11443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2975
FEATURES             Location/Qualifiers
     source          1..11443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4754..4763
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    7300..7888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7353..7373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7771..7782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7960..8193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8271..8392
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8308..8327)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8362..8381
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8432..8567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8587..8606)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8588..8606)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8616..8633)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8616..8632)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8625..8647)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8774..9229
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8861..8880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9071..9092
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9255..9359
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9360..9428,9429..10220)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9578..9597)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10391..10979
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10880..10899
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11133..11150
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11267..11288
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11303..11308,11309..11326,11327..11333)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11341..11357
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11346..11368
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11365..11381
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11365..11381
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11400..11420
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11402..11420
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcat cggaaaaagg tggataaccg
     4141 aatccggatt ctcattgaga atggagtagc tgagcggcaa agatctctct ttgttgtagt
     4201 tggggatcga ggaaaagatc aggtggtaat acttcatcac atgttatcca aagcaactgt
     4261 gaaggctcgg ccttcagtgc tgtggtgtta taagaaagag ctggggttta gcagtcaccg
     4321 gaagaaaaga atgcgacagc tgcagaagaa aataaagaat ggaacactga acataaagca
     4381 ggacgacccc tttgaactct tcatagcagc cacaaacatt cgctactgct actacaacga
     4441 gacccacaag atcctgggca ataccttcgg catgtgtgtg ctgcaggatt ttgaagcctt
     4501 aactccaaac ttgctggcca ggactgtaga aacagtggaa ggtggtgggc tagtggtcat
     4561 cctcctacgg accatgaact cactcaagca attgtacaca gtgactatgg atgtgcattc
     4621 caggtacaga actgaggccc atcaggatgt ggtgggaaga tttaatgaaa ggtttattct
     4681 gtctctggcc tcttgtaaga agtgtctcgt cattgatgac cagctcaaca tcctgcccat
     4741 ctcctcccac gttgccacca tggaggccct gcctccccag actccggatg agagtcttgg
     4801 tccttctgat ctggagctga gggagttgaa ggagagcttg caggacaccc agcctgtggg
     4861 tgtgttggtg gactgctgta agactctaga ccaggccaaa gctgtcttga aatttatcga
     4921 gggcatctct gaaaagaccc tgaggagtac tgttgcactc acagctgctc gaggacgggg
     4981 aaaatctgca gccctgggat tggcgattgc tggggcggtg gcatttgggt actccaatat
     5041 ctttgttacc tccccaagcc ctgataacct ccatactctg tttgaatttg tatttaaagg
     5101 atttgatgct ctgcaatatc aggaacatct ggattatgag attatccagt ctctaaatcc
     5161 tgaatttaac aaagcagtga tcagagtgaa tgtatttcga gaacacaggc agactattca
     5221 gtatatacat cctgcagatg ctgtgaagct gggccaggct gaactagttg tgattgatga
     5281 agctgccgcc atccccctcc ccttggtgaa gagcctactt ggcccctacc ttgttttcat
     5341 ggcatccacc atcaatggct atgagggcac tggccggtca ctgtccctca agctaattca
     5401 gcagctccgt caacagagcg cccagagcca ggtcagcacc actgctgaga ataagaccac
     5461 gacgacagcc agattggcat cagcgcggac actgtatgag gtttccctcc aggagtcaat
     5521 ccgatacgcc cctggggatg cagtggagaa gtggctgaat gacttgctgt gcctggattg
     5581 cctcaacatc actcggatag tctcaggctg ccccttgcct gaagcttgtg aactgtacta
     5641 tgttaataga gataccctct tttgctacca caaggcctct gaagttttcc tccaacggct
     5701 tatggccctc tacgtggctt ctcactacaa gaactctccc aatgatctcc agatgctctc
     5761 cgatgcacct gctcaccatc tcttctgcct tctgcctccc gtgcccccca cccagaatgc
     5821 ccttccagaa gtgcttgctg ttatccaggt gtgccttgaa ggggagattt ctcgccagtc
     5881 catcttgaac agtctgtctc gaggcaagaa ggcttcaggg gacctgattc catggacagt
     5941 gtcagaacag ttccaagatc cagactttgg tggtctgtct ggtggaaggg tcgttcgcat
     6001 tgctgttcac ccagattatc aagggatggg ctatggcagc cgtgctctgc agctgctgca
     6061 gatgtactat gaaggcaggt ttccttgtct ggaggaaaag gtccttgaga caccacagga
     6121 aattcacacc gtaagcagcg aggctgtcag cttgttggaa gaggtcatca ctccccggaa
     6181 ggacctgcct cctttactcc tcaaattgaa tgagaggcct gccgaacgcc tggattacct
     6241 gggtgtttcc tatggcttga cccccaggct cctcaagttc tggaaacgag ctggatttgt
     6301 tcctgtttat ctgagacaga ccccgaatga cctgaccgga gagcactcgt gcatcatgct
     6361 gaagacgctc actgatgagg atgaggctga ccagggaggc tggcttgcag ccttctggaa
     6421 agatttccga cggcggttcc tagccttgct ctcctaccag ttcagtacct tctctccttc
     6481 cctggctctg aacatcattc agaacaggaa catggggaag ccagcccagc ctgccctgag
     6541 ccgggaggag ctggaagcac tcttcctccc ctatgacctg aagcggctgg agatgtattc
     6601 acggaatatg gtggactatc acctcatcat ggacatgatc ccggccatct ctcgcatcta
     6661 tttcctgaac cagctggggg acctggccct gtctgcggct cagtcggctc ttctcttggg
     6721 gattggcctg cagcataagt ctgtggacca gctggaaaag gagattgagc tgccctcggg
     6781 ccagttgatg ggacttttca accggatcat ccgcaaagtt gtgaagctat ttaatgaagt
     6841 tcaggaaaag gccattgagg agcagatggt ggcagcgaag gatgtggtca tggagcccac
     6901 gatgaagacc ctcagtgacg acctagatga agcagcaaag gaatttcagg agaaacacaa
     6961 gaaggaagta gggaagctga agagcatgga cctctctgaa tacataatcc gtggggacga
     7021 tgaagagtgg aatgaagttt tgaacaaagc tgggccgaac gcctcgatca tcagcctgaa
     7081 aagtgacaag aaaaggaagt tagaggccaa acaagaaccc aaacagagca agaagttgaa
     7141 gaacagagag acaaagaaca aaaaagatat gaaactgaag cggaagaaat agtaatgaac
     7201 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     7261 gcacgaatcg tcgttatcgt atggacgcgt taagtcgaca atcaacctct ggattacaaa
     7321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     7381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     7441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     7501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     7561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     7621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     7681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     7741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     7801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     7861 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     7921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7981 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     8041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     8101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     8161 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     8221 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     8281 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8341 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     8401 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     8461 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     8521 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     8581 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     8641 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     8701 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     8761 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     8821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     8881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     8941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     9001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9061 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     9121 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     9181 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     9241 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     9301 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     9361 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     9421 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     9481 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     9541 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     9601 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     9661 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     9721 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     9781 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     9841 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     9901 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     9961 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
    10021 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
    10081 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
    10141 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
    10201 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
    10261 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
    10321 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
    10381 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
    10441 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
    10501 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
    10561 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
    10621 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
    10681 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
    10741 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
    10801 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
    10861 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
    10921 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
    10981 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
    11041 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
    11101 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
    11161 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
    11221 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
    11281 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
    11341 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
    11401 caattaaccc tcactaaagg gaacaaaagc tggagctgca agc
//
