LOCUS       sequence-407376-        9816 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Crimean-Congo hemorrhagic fever virus GP 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9816)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 9816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_CCFH_GP
FEATURES             Location/Qualifiers
     source          1..9816
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4302..4311
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6521..6540)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6586..6641
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6640..6659)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6999..7015)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6999..7015)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7012..7034)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7023..7039
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7047..7053,7054..7071,7072..7077))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7092..7113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7230..7247)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7401..7989)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7481..7500)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8160..8951,8952..9020))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8783..8802
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9021..9125)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9151..9606)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9288..9309)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9500..9519
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9733..9755
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9747..9764
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9748..9764
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9774..9793
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9774..9792
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgcac atctctctga
     1441 tgtacgccat tctgtgtctg cagctctgcg gactgggaga aacacatggc agccacaacg
     1501 aaacaaggca caataaaacc gacacaatga ccacacccgg cgataacccc agctccgagc
     1561 ctcccgtgtc caccgctctg agcattacac tggatcctag caccgtgaca cccacaacac
     1621 ccgccagcgg actggaagga agcggagaag tctacaccag ccctcccatt acaaccggct
     1681 ctctgcctct ctccgagaca acccccgagc tccccgtgac caccggcacc gatacactgt
     1741 ccgccggaga tgtcgaccct agcacccaga cagccggagg aaccagcgcc cctacagtga
     1801 gaaccagcct ccccaatagc ccctccaccc ccagcacacc tcaagacaca caccatcccg
     1861 tgaggaatct gctgtccgtg acaagccccg gccccgatga gacaagcacc cctagcggaa
     1921 ccggaaaaga gtcctccgcc accagcagcc ctcaccccgt gagcaataga ccccctacac
     1981 cccccgccac agcccaaggc cccaccgaga acgacagcca caatgctacc gagcatcccg
     2041 agtctctgac acagagcgct acacccggac tgatgacatc ccccacacag attgtgcatc
     2101 cccagagcgc cacccctatc acagtgcaag acacacatcc ctcccccacc aataggagca
     2161 agaggaatct gaaaatggag atcattctca cactgagcca aggcctcaag aagtactatg
     2221 gaaaaattct gagactgctg cagctgaccc tcgaggagga cacagaggga ctgctcgagt
     2281 ggtgcaaaag aaatctggga ctggactgcg atgacacctt cttccaaaaa aggattgaag
     2341 agttcttcat caccggagaa ggccacttca acgaagtgct gcagtttaga acccccggca
     2401 cactgagcac cacagagtcc acccccgctg gactccctac agccgaaccc tttaagtcct
     2461 actttgctaa gggcttcctc agcatcgatt ccggctatta ctccgccaaa tgttacagcg
     2521 gcacatccaa ctccggactg cagctgatca acatcacaag gcactccaca agaattgtcg
     2581 acacccccgg ccctaagatt accaacctca agaccattaa ctgtattaat ctgaaggcca
     2641 gcatctttaa ggaacatagg gaggtggaga tcaatgtgct gctcccccaa gtggctgtga
     2701 atctgagcaa ctgccatgtc gtcatcaaat cccacgtctg cgactatagc ctcgacatcg
     2761 atggcgctgt gagactgcct cacatttacc atgagggcgt gttcattccc ggcacatata
     2821 aaatcgtgat cgataagaaa aacaagctca acgacagatg tacactgttc accgactgcg
     2881 tgattaaggg aagagaggtg agaaaaggcc agtccgtgct gagacagtac aagaccgaga
     2941 tcagaatcgg caaagcttcc accggcagca gaaggctgct gtccgaggaa ccctccgatg
     3001 actgtatttc tagaacccag ctgctgagaa ccgaaacagc cgagatccac ggagacaatt
     3061 atggcggacc cggcgacaag attaccatct gtaacggatc cacaatcgtg gatcaaaggc
     3121 tcggatccga gctcggctgc tatacaatca atagagtcag atcctttaag ctgtgtgaga
     3181 actccgccac cggaaagaat tgtgagatcg acagcgtccc cgtgaaatgt agacaaggct
     3241 actgtctgag gatcacacaa gagggaagag gacacgtcaa gctcagcaga ggatccgagg
     3301 tcgtgctcga cgcttgcgat accagctgcg agatcatgat tcctaagggc accggcgaca
     3361 ttctggtcga ctgttccggc ggacaacagc atttcctcaa agataatctg atcgatctgg
     3421 gatgccccaa aatccctctg ctgggcaaga tggccattta catttgcaga atgagcaacc
     3481 accccaagac cacaatggct tttctgttct ggttcagctt cggctacgtc attacatgta
     3541 ttctgtgcaa ggccatcttc tatctgctga tcattgtcgg aacactcggc aagaggctca
     3601 aacagtatag agaactcaag ccccagacat gcacaatttg cgagaccacc cccgtgaacg
     3661 ctattgatgc tgagatgcac gatctgaact gtagctacaa catttgtccc tattgcgcta
     3721 gcagactcac ctccgacgga ctggctagac atgtcatcca gtgccctaag agaaaggaga
     3781 aggtcgaaga gaccgagctg tatctgaacc tcgagaggat cccttgggtc gtgaggaaac
     3841 tgctgcaagt gagcgagagc accggcgtgg ctctcaagag aagcagctgg ctcatcgtgc
     3901 tgctcgtgct gtttaccgtg tccctcagcc ccgtgcagag cgctcccatt ggacaaggca
     3961 aaaccatcga ggcttacaga gccagagagg gctacaccag catttgtctg tttgtgctcg
     4021 gctccatcct cttcattgtc agctgtctga tgaagggact ggtggatagc gtgggaaaca
     4081 gctttttccc cggcctcagc atctgcaaga catgctccat tagcagcatc aatggatttg
     4141 aaatcgaaag ccacaaatgt tattgctctc tgttctgctg tccctactgt agacattgtt
     4201 ccaccgacaa ggagatccac aagctccatc tgagcatctg caaaaaaagg aagaagggca
     4261 gcaatgtgat gctggctgtc tgcaaactga tgtgcttcag agccaccatg gaggtctcca
     4321 acagagctct cttcattaga tccatcatta acacaacctt cgtgctgtgt attctgatcc
     4381 tcgctgtgtg cgtggtcagc acatccgctg tcgagatgga gaacctcccc gccggcacat
     4441 gggaaagaga ggaggatctg accaacttct gccaccaaga gtgtcaagtg accgaaacag
     4501 agtgcctctg cccttacgaa gccctcgtgc tgagaaagcc cctctttctg gatagcaccg
     4561 ccaagggcat gaaaaacctc ctcaacagca ccagcctcga aacatctctg agcattgaag
     4621 ctccttgggg cgccatcaac gtgcaatcca cctacaagcc caccgtcagc accgccaaca
     4681 tcgccctctc ttggagctcc gtcgagcaca gaggaaacaa aattctggtc tccggaagga
     4741 gcgagtccat catgaaactc gaggagagaa ccggaatcag ctgggacctc ggcgtcgagg
     4801 acgcttccga atccaagctc ctcacagtct ccgtcatgga tctgtcccag atgtatagcc
     4861 ccgtgtttga gtatctgtcc ggcgataggc aagtgggcga atggcccaaa gccacatgca
     4921 ccggagattg ccccgagaga tgcggctgta catccagcac atgtctgcac aaggaatggc
     4981 cccattctag aaattggagg tgcaacccca catggtgctg gggagtcggc accggctgca
     5041 catgctgcgg actggatgtg aaggacctct tcacagatta tatgtttgtc aaatggaagg
     5101 tggagtacat caaaaccgag gctattgtct gtgtggagct gaccagccaa gagagacagt
     5161 gcagcctcat cgaagccgga acaaggttca acctcggccc cgtcaccatt acactgagcg
     5221 agcctaggaa tatccagcag aagctgcctc ccgaaatcat caccctccat cctagaatcg
     5281 aggaaggctt ttttgatctc atgcacgtgc agaaagtgct gtccgctagc accgtgtgta
     5341 aactgcagag ctgtacacac ggagtccccg gcgatctgca agtctatcac attggcaatc
     5401 tgctgaaggg agacaaagtc aacggccatc tcatccataa aatcgagcct catttcaaca
     5461 caagctggat gagctgggat ggatgcgacc tcgactacta ttgcaacatg ggcgactggc
     5521 ctagctgtac ctataccggc gtgacccagc acaatcacgc ttccttcgtc aatctgctga
     5581 acatcgagac cgattacaca aaaaactttc acttccacag caagagagtc acagcccatg
     5641 gcgacacccc ccagctcgat ctcaaagcta gacccacata tggcgctggc gagatcaccg
     5701 tgctcgtcga ggtcgccgac atggaactcc acaccaagaa aatcgaaatt agcggactga
     5761 agttcgcttc tctggcttgc accggctgtt atgcttgcag ctccggaatt agctgtaagg
     5821 tgaggatcca cgtcgacgag cccgacgaac tgaccgtcca tgtgaagtcc gacgaccccg
     5881 atgtcgtcgc tgctagcagc agcctcatgg ctagaaagct cgagttcggc acagacagca
     5941 catttaaagc cttcagcgcc atgcctaaaa catctctgtg cttctacatc gtggaaaggg
     6001 agcactgcaa gagctgctcc gaggaagata caaagaagtg cgtgaatacc aagctcgagc
     6061 agcctcagag cattctgatt gagcacaaag gaaccattat cggcaaacaa aattccacat
     6121 gcaccgccaa ggccagctgc tggctggaat ccgtgaagtc cttcttctac ggactgaaga
     6181 acatgctgag cggcatcttc ggcaatgtct ttatgggcat ctttctgttt ctggccccct
     6241 ttattctgct cattctgttc ttcatgttcg gatggagaat cctcttttgc tttaagtgct
     6301 gcagaagaac aagaggactg ttcaagtata gacatctcaa agacgacgag gaaaccggct
     6361 atagaaggat tattgaaaag ctgaataata aaaaaggaaa gaacaagctg ctcgacggag
     6421 aaaggctcgc cgacagaaga atcgctgagc tgttcagcac caaaacccac atcggctgat
     6481 gtcttcctgg agctccagtc attgactata acgtctcaga tctttttccc tctgccaaaa
     6541 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     6601 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     6661 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     6721 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     6781 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     6841 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     6901 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     6961 ggagatccct cgacggatcg gccgcaattc gtaatcatgt catagctgtt tcctgtgtga
     7021 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7081 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7141 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7201 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7261 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7321 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7381 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7441 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7501 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7561 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7621 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7681 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7741 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7801 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7861 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7921 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7981 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8041 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8101 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8161 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8221 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8281 agtgctgcaa tgataccgcg agacccacgc tcaccggcac cagatttatc agcaataaac
     8341 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8401 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8461 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8521 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8581 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8641 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8701 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8761 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8821 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8881 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8941 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9001 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9061 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9121 ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt ttgttaaaat
     9181 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     9241 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     9301 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     9361 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     9421 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     9481 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     9541 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     9601 gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     9661 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     9721 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
     9781 actcactata gggcgaattg gagctccacc gcggtg
//
