LOCUS       sequence-407383-        6462 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Influenza A virus HA protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_FLUAV_HA
FEATURES             Location/Qualifiers
     source          1..6462
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3167..3186)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3232..3287
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3286..3305)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3645..3661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3645..3661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3658..3680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3669..3685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3693..3699,3700..3717,3718..3723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3738..3759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3876..3893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4047..4635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4127..4146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4806..5597,5598..5666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5429..5448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5667..5771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5797..6252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5934..5955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6146..6165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6379..6401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6393..6410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6394..6410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6420..6439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6420..6438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgaag gctaatctgc
     1441 tggtgctgct ctgtgctctg gctgccgctg acgccgacac aatctgtatc ggctaccatg
     1501 ctaacaacag caccgatacc gtggataccg tgctcgagaa gaacgtgacc gtgacacact
     1561 ccgtgaatct gctcgaggac tcccataatg gcaagctctg tagactgaaa ggaatcgctc
     1621 ctctgcagct cggaaaatgt aacatcgccg gatggctgct gggcaatccc gagtgcgatc
     1681 ctctgctccc cgtgagaagc tggtcctaca tcgtggaaac acccaactcc gagaatggca
     1741 tttgctaccc cggcgacttc atcgactatg aggagctgag agagcaactc tcctccgtgt
     1801 cctccttcga gaggttcgaa atcttcccta aggagtccag ctggcccaac cacaatacaa
     1861 ccaagggagt caccgctgct tgtagccacg ctggcaagtc cagcttctat agaaatctgc
     1921 tctggctcac agagaaggag ggcagctatc ccaaactgaa aaacagctac gtgaataaga
     1981 agggaaagga ggtgctggtg ctctggggca tccaccaccc ctccaactcc aaggaccaac
     2041 aaaacattta ccagaacgag aacgcctatg tgagcgtcgt cacctccaac tacaatagga
     2101 ggttcacccc cgaaatcgcc gagaggccta aggtgaggga tcaagccggc agaatgaact
     2161 actactggac actgctcaag cccggcgaca caatcatctt cgaggccaat ggcaatctga
     2221 tcgctcctag atacgctttc gctctgagca gaggcttcgg cagcggaatc attaccagca
     2281 acgcctccat gcacgagtgc aacaccaagt gtcaaacccc tctgggagcc attaatagca
     2341 gcctcccctt ccagaatatc catcccgtga ccatcggcga gtgtcccaaa tacgtgagga
     2401 gcgccaagct gaggatggtg accggactga gaaacatccc tagcatccaa tctagaggac
     2461 tgtttggcgc tatcgctggc ttcatcgaag gcggctggac cggaatgatc gatggctggt
     2521 acggctacca ccaccagaat gaacaaggaa gcggctacgc cgctgaccag aagtccacac
     2581 agaacgccat caacggcatc accaacaagg tcaacagcgt gattgaaaag atgaatatcc
     2641 agttcacagc cgtcggcaag gagtttaata agctcgagaa gagaatggag aatctgaaca
     2701 agaaagtgga cgacggcttt ctggacatct ggacctataa cgccgagctg ctcgtgctgc
     2761 tcgagaatga aaggacactg gattttcatg actccaacgt gaaaaatctg tacgagaagg
     2821 tgaaaagcca gctcaaaaat aacgccaaag aaatcggcaa cggctgtttt gagttttacc
     2881 ataaatgcga caacgagtgc atggagtccg tgaggaacgg aacctacgac tatcccaagt
     2941 acagcgagga aagcaagctg aatagagaaa aggtggacgg cgtgaaactc gagtccatgg
     3001 gcatttacca gatcctcgcc atctactcca cagtcgcttc ctctctggtg ctcctcgtgt
     3061 ctctgggagc catctccttt tggatgtgca gcaatggctc tctgcagtgt agaatttgca
     3121 tctgatgtct tcctggagct ccagtcattg actataacgt ctcagatctt tttccctctg
     3181 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     3241 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     3301 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     3361 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     3421 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     3481 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     3541 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     3601 tcttatggag atccctcgac ggatcggccg caattcgtaa tcatgtcata gctgtttcct
     3661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4561 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4621 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4681 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4741 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4801 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4861 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4921 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggcaccaga tttatcagca
     4981 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5041 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5101 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5161 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5221 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5281 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5341 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5401 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5461 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5521 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5581 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5641 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5701 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5761 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     5821 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     5881 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     5941 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6001 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6061 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6121 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6181 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6241 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6301 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6361 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     6421 aatacgactc actatagggc gaattggagc tccaccgcgg tg
//
