LOCUS       sequence-407385-        6126 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Lujo virus GPC protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_LUHF_GPC
FEATURES             Location/Qualifiers
     source          1..6126
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2831..2850)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2896..2951
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2950..2969)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3309..3325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3309..3325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3322..3344)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3333..3349
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3357..3363,3364..3381,3382..3387))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3402..3423
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3540..3557)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3711..4299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3791..3810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4470..5261,5262..5330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5093..5112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5331..5435)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5461..5916)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5598..5619)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5810..5829
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6043..6065
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6057..6074
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6058..6074
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6084..6103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6084..6102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgggc caaattgtgg
     1441 ccgtgttcca agccatcccc gagattctga acgaggccat caacatcgtc atcatcgtga
     1501 ttattatgtt tacactgatt aaaggcgtgt ttaatctgta caagtccgga ctgttccagc
     1561 tcgtgatctt tctgctgctg tgtggcaaaa ggtgcgacag ctctctgctg agcggcttca
     1621 acctcgagac cgtgcacttc aacatgtctc tgctgtccag catccctatg gtgagcgaac
     1681 agcagcactg catccagcac aaccacagca gcattacctt ctctctgctc accaacaagt
     1741 ccgacctcga gaagtgcaac tttacaaggc tgcaagccgt ggatagagtg atcttcgatc
     1801 tgtttagaga gtttcaccac agagtcggag acttccccgt caccagcgat ctgaagtgta
     1861 gccacaacac cagctataga gtgatcgagt acgaggtcac aaaggagtct ctgcccagac
     1921 tgcaagaggc tgtgagcacc ctctttcccg atctgcatct gagcgaggac agatttctgc
     1981 agatccaagc ccacgacgac aagaactgca ccggactgca ccctctcaac tatctgagac
     2041 tgctgaagga gaacagcgag acacactaca aggtgaggaa gctgatgaag ctcttccagt
     2101 ggtctctgtc cgatgaaacc ggctcccctc tgcccggcgg ccattgcctc gagagatggc
     2161 tgatcttcgc tagcgacatc aagtgcttcg ataacgccgc catcgctaag tgcaacaagg
     2221 agcatgatga ggagttctgc gacatgctca gactcttcga ctacaacaag gccagcatcg
     2281 ctaagctgag gggagaagct agcagcagca tcaatctgct gtccggaaga atcaacgcta
     2341 tcatctccga cacactgctc atgagaagca gcctcaagag gctgatgggc atcccctact
     2401 gtaactacac caagttctgg tatctgaatc acaccaagct gggcatccac tctctgccca
     2461 gatgctggct ggtgtccaat ggcagctacc tcaatgaaac aaagttcaca cacgacatgg
     2521 aggacgaagc tgacaaactg ctcaccgaaa tgctcaagaa ggagtacgtg agaaggcaag
     2581 aaaagacccc cattaccctc atggacattc tgatgttctc tgtgagcttc tacatgttca
     2641 gcgtgaccct ctgcatttgt aacatcccca cccacagaca tatcaccgga ctgccttgtc
     2701 ctaagcctca tagactgaga aagaacggca catgcgcttg cggcttcttc aagagcatca
     2761 atagaagcac cggctgggcc aaacactgat gtcttcctgg agctccagtc attgactata
     2821 acgtctcaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     2881 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     2941 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     3001 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     3061 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     3121 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     3181 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     3241 tcccagtcat agctgtccct cttctcttat ggagatccct cgacggatcg gccgcaattc
     3301 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3361 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3421 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3481 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     3541 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     3601 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     3661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     3721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     3781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     3841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     4561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     4621 tcaccggcac cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     4681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     4741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5461 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     5521 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     5581 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     5641 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     5701 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     5761 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     5821 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     5881 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     5941 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     6001 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     6061 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc
     6121 gcggtg
//
