LOCUS       sequence-407386-        6273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Variegated Squirrel Bornavirus G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6273)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_VSBV_G
FEATURES             Location/Qualifiers
     source          1..6273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(2978..2997)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3043..3098
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3097..3116)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3456..3472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3456..3472)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3469..3491)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3480..3496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3504..3510,3511..3528,3529..3534))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3549..3570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3687..3704)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3858..4446)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3938..3957)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4617..5408,5409..5477))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5240..5259
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5478..5582)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5608..6063)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5745..5766)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5957..5976
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6190..6212
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6204..6221
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6205..6221
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6231..6250
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6231..6249
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgcag agcacacagc
     1441 actccatgag ctttcccatc gtctgtgtca ttctgggaag cgtcctcctc gtgcagacat
     1501 tcgaactgag aggactgaga tgtaacaccg attccacccc cgctctggtg gatctcgaaa
     1561 tcagaaggct gtgccacacc cccagcgaaa atgtcattag ctgcgaggtc tcctactaca
     1621 accattccca gcaaagcctc gaggccagac ataccagctg ctataaatac cactgcaaaa
     1681 cctactgggg cttcttcggc tcctacagcg ctgataggct ggtgagcaga tacaccggcg
     1741 cctttaacgt gtgcaataac ggctccttcc ccgacgaccc ctatgaatgt aactggtatt
     1801 actgctgtag cgctctcacc accgaaatct gcagatgcag catcaataac gtcaccgtgg
     1861 ccgtgaggac ctttccccct ttcatgtact gtagcttcgc tgactgcagc accgtgtccc
     1921 agctcgaact cacaaccggc tttgccagcc tcagcgacgg ctccgtgctc gagttcgagc
     1981 cttggaacat ctccaaggaa ctcatcaacg gcaccttcaa tggaacaatt ctgtgcaact
     2041 ctagcagcaa gatcatcagc tttgacgagt tcagaaggag ctactctctg gtcaacggca
     2101 catatatgtc cagctccatc aacatcacat gctcctccta caacggcaca tgcagagcca
     2161 aacctagaaa gaaaagggat ctgagccaga tcgaatatct ggtgcacaaa ctgagaccca
     2221 cactgaggga cgcttgggaa gattgcgaaa ttctgcagtc tctgctgctg ggagtgttcg
     2281 gcaccggaat ggccagcgct agccagtttc tgagggagtg gctcaaccag accaacattg
     2341 tcggctatgt cgtcaatgga gtgggagtgg tctggcagtg tgattgggtg aatgtgagct
     2401 ttctgccttg gaacgagagc acctactatc cccccgtgaa ctttagcaac tctagactgt
     2461 atctgaacga cgagtctaga ctgcagacct ccacccccga agccagaccc ggactgaaga
     2521 gagtgctctg gttcggaaga tattttctgg gcacagtggg cagcggcgtc aagcctagaa
     2581 ggatcagata caatagaacc agccacgact tccatctgca agactttgac gcctctctga
     2641 atctgacccc tagcattaat ctggccaccg gccacgaaac agatcccatc aatcacgcct
     2701 tcggcaccca agccaatctg ctgccctaca caagatccaa taacatcacc agcaccgaca
     2761 ccggaagcgg atgggtgcat atcggactgc cctcctttgc ctttctgaac cctctgggct
     2821 ggctcagaga tatcttcagc tgggccgctt ggattggcgg cattctgtat ctgatcacac
     2881 tgtgtatctc tctgcccgcc tcctttgcca gaaggaggag gctgggaaga tggtgatgtc
     2941 ttcctggagc tccagtcatt gactataacg tctcagatct ttttccctct gccaaaaatt
     3001 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3061 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3121 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     3181 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     3241 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3301 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3361 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     3421 gatccctcga cggatcggcc gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     3481 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3541 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3601 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3661 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3781 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4741 gctgcaatga taccgcgaga cccacgctca ccggcaccag atttatcagc aataaaccag
     4801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4921 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5221 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5581 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5641 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5701 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5761 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5821 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5881 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5941 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6001 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6061 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6121 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6181 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     6241 cactataggg cgaattggag ctccaccgcg gtg
//
