LOCUS       sequence-407387-        8169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Hantaan virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8169)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_HTNV_GP
FEATURES             Location/Qualifiers
     source          1..8169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4874..4893)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4939..4994
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4993..5012)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5352..5368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5352..5368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5365..5387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5376..5392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5400..5406,5407..5424,5425..5430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5445..5466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5583..5600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5754..6342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5834..5853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6513..7304,7305..7373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7136..7155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7374..7478)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7504..7959)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7641..7662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7853..7872
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8086..8108
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8100..8117
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8101..8117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8127..8146
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8127..8145
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgggc atctggaagt
     1441 ggctggtcat ggcttctctg gtctggcccg tgctgacact gagaaacgtg tacgacatga
     1501 agatcgaatg cccccacacc gtgtcctttg gagagaacag cgtgatcggc tatgtggagc
     1561 tccctcccgt gcctctggcc gatacagctc aaatggtccc cgagtccagc tgcaacatgg
     1621 ataaccacca gagcctcaac accatcacca agtataccca agtgtcttgg agaggcaaag
     1681 ctgaccaaag ccagtcctcc cagaactcct tcgagacagt gagcaccgag gtggacctca
     1741 agggcacatg cgtgctgaaa cataagatgg tcgaggagag ctataggagc agaaagagcg
     1801 tcacatgcta cgatctcagc tgcaactcca cctattgcaa gcccacactg tatatgattg
     1861 tgcccatcca tgcttgtaat atgatgaaga gctgcctcat tgccctcggc ccctacagag
     1921 tccaagtcgt gtacgagaga tcctattgca tgaccggagt cctcattgaa ggcaagtgtt
     1981 tcgtgcccga ccagtccgtc gtcagcatta tcaagcacgg catcttcgac atcgccagcg
     2041 tccacatcgt gtgtttcttt gtggccgtca aaggcaacac ctacaaaatc ttcgagcaag
     2101 tgaagaaatc cttcgagtcc acatgcaacg ataccgagaa taaggtccaa ggctattata
     2161 tttgcatcgt gggcggaaat tccgctccca tttatgtccc caccctcgac gactttagat
     2221 ccatggaggc ctttaccggc attttcagat cccctcatgg cgaggatcac gatctggccg
     2281 gcgaggaaat tgcctcctac tccatcgtcg gccccgccaa tgccaaggtg cctcactccg
     2341 ctagctccga cacactgtct ctgattgcct actccggcat ccccagctat agctccctca
     2401 gcattctgac cagctccacc gaagctaagc atgtgttctc ccccggactg ttccctaagc
     2461 tcaaccacac caactgcgac aaaagcgcca ttcctctgat ttggaccggc atgatcgacc
     2521 tccccggata ttatgaagct gtccatcctt gcaccgtgtt ctgcgtgctc tccggccccg
     2581 gcgccagctg cgaggccttc tccgagggag gaattttcaa cattaccagc cctatgtgtc
     2641 tcgtgtccaa gcaaaataga tttagactga ccgagcagca agtgaatttt gtgtgccaga
     2701 gggtggatat ggacatcgtc gtgtactgta atggccagag aaaggtgatc ctcacaaaga
     2761 cactcgtgat tggccagtgc atctacacca ttacctctct gttcagcctc ctccccggcg
     2821 tggcccactc catcgccgtg gaactgtgtg tgcccggctt tcatggctgg gccaccgctg
     2881 ctctgctggt cacattctgt ttcggatggg tgctgatccc cgccattaca ttcatcatcc
     2941 tcaccgtcct caaatttatt gccaacatct ttcataccag caatcaagag aataggctca
     3001 aatccgtgct gaggaagatc aaggaggagt tcgagaaaac caaaggctcc atggtctgcg
     3061 acgtctgcaa gtacgagtgc gagacataca aagagctcaa agcccacgga gtcagctgcc
     3121 ctcagtccca gtgcccctat tgctttacac attgtgagcc caccgaagcc gcctttcaag
     3181 cccactacaa agtgtgccaa gtcacacata gatttaggga cgatctgaag aaaaccgtga
     3241 ccccccagaa ctttaccccc ggctgctata gaacactgaa tctgtttagg tacaaatcta
     3301 gatgttacat cttcaccatg tggatctttc tgctcgtgct cgagagcatt ctgtgggccg
     3361 ctagcgctag cgagacccct ctgacccccg tgtggaacga caacgctcac ggagtcggat
     3421 ccgtccccat gcacaccgat ctcgagctgg atttctccct cacctctagc agcaagtaca
     3481 catatagaag gaagctgacc aaccctctgg aagaagccca gagcatcgat ctgcatattg
     3541 agatcgagga gcagaccatt ggagtcgacg tccatgccct cggccattgg ttcgacggca
     3601 gactgaacct caagaccagc ttccactgtt atggcgcttg caccaagtac gagtaccctt
     3661 ggcataccgc taaatgtcac tacgaaaggg actaccagta cgagacaagc tggggatgta
     3721 accctagcga ttgccccggc gtgggcaccg gctgcacagc ttgtggactg tacctcgatc
     3781 agctgaagcc cgtcggctcc gcctacaaaa ttattacaat tagatatagc agaagagtgt
     3841 gcgtccaatt cggagaggaa aatctgtgta aaatcatcga catgaatgac tgctttgtga
     3901 gcagacacgt gaaagtctgc atcatcggca cagtgagcaa gttcagccaa ggcgacacac
     3961 tgctgttctt cggacctctc gaaggaggcg gactgatctt caagcattgg tgtacctcca
     4021 catgccagtt cggcgacccc ggcgacatta tgagccccag agacaagggc ttcctctgtc
     4081 ccgagttccc cggctccttt agaaaaaagt gtaacttcgc taccaccccc atctgcgaat
     4141 acgacggcaa tatggtgagc ggctataaga aggtcatggc caccattgat agcttccagt
     4201 ccttcaatac aagcacaatg cacttcaccg acgagagaat cgaatggaaa gaccccgacg
     4261 gaatgctgag ggaccacatt aacattctgg tcaccaagga tatcgatttc gacaacctcg
     4321 gagagaatcc ttgcaaaatc ggactgcaga caagcagcat tgagggagct tggggctccg
     4381 gagtcggctt cacactcaca tgtctggtgt ctctgaccga atgtcccacc tttctgacca
     4441 gcatcaaggc ttgcgacaag gctatttgct acggagctga atccgtgaca ctgaccagag
     4501 gccagaatac agtgaaggtc agcggaaagg gaggccatag cggctccaca tttaggtgtt
     4561 gccacggaga ggactgctcc cagattggac tgcatgccgc tgccccccat ctggataagg
     4621 tgaacggcat ctccgaaatc gagaacagca aggtgtatga cgatggcgct cctcagtgcg
     4681 gaatcaagtg ctggtttgtg aaatccggcg agtggatcag cggcatcttc agcggcaact
     4741 ggattgtcct catcgtgctc tgtgtgttcc tcctcttttc tctggtgctg ctgagcattc
     4801 tgtgtcccgt gagaaagcat aagaagagct gatgtcttcc tggagctcca gtcattgact
     4861 ataacgtctc agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     4921 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     4981 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     5041 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     5101 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     5161 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     5221 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     5281 tactcccagt catagctgtc cctcttctct tatggagatc cctcgacgga tcggccgcaa
     5341 ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5401 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5461 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5521 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5581 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5641 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5941 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6181 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6661 cgctcaccgg caccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7381 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7501 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     7561 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     7621 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     7681 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     7741 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     7801 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     7861 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     7921 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     7981 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     8041 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     8101 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc
     8161 accgcggtg
//
