LOCUS       sequence-407393-        6825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of St. Louis encephalitis virus PrME protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_SLEV_PrME
FEATURES             Location/Qualifiers
     source          1..6825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3530..3549)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3595..3650
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3649..3668)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4008..4024)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4008..4024)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4021..4043)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4032..4048
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4056..4062,4063..4080,4081..4086))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4101..4122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4239..4256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4410..4998)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4490..4509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5169..5960,5961..6029))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5792..5811
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6030..6134)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6160..6615)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6297..6318)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6509..6528
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6742..6764
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6756..6773
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6757..6773
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6783..6802
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6783..6801
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgggc ggaacaaggt
     1441 ctctgctggg actggctgct ctgattggac tggccagctc tctgcagctg tccacctacc
     1501 aaggcaaggt gctcatgtcc atcaacaaga ccgacgctca atccgccatc aacatccctt
     1561 ccgccaacgg cgccaataca tgcatcgtga gagctctgga cgtcggcgtc atgtgtaagg
     1621 acgatatcac ctatctgtgc cccgtgctgt ccgccggcaa tgatcccgag gacattgact
     1681 gttggtgcga cgtggaagag gtctgggtgc attacggcag atgcacaaga atgggacaca
     1741 gcagaaggtc tagaagaagc atcagcgtgc agcaccacgg agactccaca ctcgccacca
     1801 agaatacacc ttggctggac acagtcaaga ccaccaagta tctcaccaaa gtggagaact
     1861 gggtgctgag aaaccccggc tatgctctcg tcgctctggc cattggctgg atgctcggaa
     1921 gcaacaacac ccagagggtg gtgttcgtga ttatgctgat gctcatcgct cccgcctatt
     1981 ccttcaactg tctgggaacc agcaatagag actttgtgga gggagcttcc ggcgctacat
     2041 ggattgatct ggtgctcgag ggaggaagct gcgtgaccgt gatggccccc gagaagccca
     2101 ccctcgattt caaggtgatg aagatggaag ctaccgagct ggccaccgtg agagagtact
     2161 gctatgaggc tacactggat acactgagca ccgtcgctag atgccccaca accggagagg
     2221 ctcacaacac aaagagatcc gatcccacct tcgtgtgcaa aagagatgtc gtcgacagag
     2281 gctggggcaa tggctgcggc ctctttggca agggatccat tgacacatgc gccaagttta
     2341 catgcaagaa caaggccacc ggcaaaacaa ttctgagaga gaacatcaaa tacgaggtgg
     2401 ccatctttgt gcacggaagc accgacagca caagccatgg caactactcc gagcaaatcg
     2461 gaaagaacca agccgctaga ttcacaatct cccctcaagc cccttccttc accgccaaca
     2521 tgggagagta cggcacagtg accatcgatt gcgaggccag aagcggaatt aacaccgagg
     2581 actactacgt ctttaccgtg aaggagaaaa gctggctcgt gaatagagat tggttccacg
     2641 atctgaatct gccttggacc tcccccgcca caacagattg gagaaacaga gagacactgg
     2701 tggagttcga ggagccccac gctaccaagc aaaccgtcgt ggctctcggc tcccaagaag
     2761 gagctctgca caccgctctg gccggagcta ttcccgccac cgtgtctagc agcacactga
     2821 cactgcaatc cggacatctg aaatgtagag ccaagctgga caaagtgaag atcaagggaa
     2881 ccacctacgg catgtgcgat agcgccttca cctttagcaa gaaccccacc gacaccggac
     2941 acggcaccgt gattgtggaa ctgcagtaca ccggctccaa cggcccttgc agagtcccta
     3001 tcagcgtgac cgccaatctg atggatctga cacccgtcgg cagactggtc accgtgaacc
     3061 ccttcatcag caccggcggc gccaacaata aggtcatgat cgaggtggag cctccttttg
     3121 gagacagcta tatcgtggtg ggaaggggca ccacacagat caattaccac tggcacaagg
     3181 agggatccag catcggcaag gctctggcca ccacatggaa gggagcccag agactggccg
     3241 tgctgggaga tacagcttgg gacttcggaa gcatcggagg cgtgtttaac agcattggca
     3301 aggccgtcca ccaagtgttc ggaggcgctt ttagaacact gtttggcggc atgagctgga
     3361 tcacccaagg actgctcgga gctctgctgc tgtggatggg actgcaagct agagacagat
     3421 ccatctctct gacactgctg gctgtgggcg gcattctgat cttcctcgcc acaagcgtgc
     3481 aagcctgatg tcttcctgga gctccagtca ttgactataa cgtctcagat ctttttccct
     3541 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     3601 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     3661 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     3721 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     3781 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     3841 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     3901 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     3961 ttctcttatg gagatccctc gacggatcgg ccgcaattcg taatcatgtc atagctgttt
     4021 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4081 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     4141 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     4201 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     4261 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4321 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4381 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4441 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4501 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4561 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4621 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4681 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4741 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4801 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4861 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4921 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4981 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     5041 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     5101 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5161 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5221 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5281 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggcacc agatttatca
     5341 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5401 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5461 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5521 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5581 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5641 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5701 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5761 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5821 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5881 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5941 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     6001 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     6061 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6121 ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt
     6181 tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa
     6241 tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag
     6301 tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg
     6361 tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga
     6421 ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg
     6481 gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg
     6541 cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc
     6601 cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
     6661 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
     6721 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
     6781 cgtaatacga ctcactatag ggcgaattgg agctccaccg cggtg
//
