LOCUS       sequence-407401-        6252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Machupo virus GPC protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6252)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_MACV_GPC
FEATURES             Location/Qualifiers
     source          1..6252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2957..2976)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3022..3077
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3076..3095)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3435..3451)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3435..3451)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3448..3470)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3459..3475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3483..3489,3490..3507,3508..3513))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3528..3549
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3666..3683)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3837..4425)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3917..3936)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4596..5387,5388..5456))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5219..5238
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5457..5561)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5587..6042)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5724..5745)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5936..5955
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6169..6191
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6183..6200
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6184..6200
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6210..6229
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6210..6228
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga cagctgatct
     1441 ccttcttcca agagatcccc gtctttctgc aagaggctct gaatattgct ctggtggctg
     1501 tgtctctgat cgccgtcatc aagggaatca tcaacctcta caaaagcgga ctgttccagt
     1561 tcattttctt tctgctgctg gccggaagaa gctgcagcga cggcacattc aagatcggac
     1621 tgcacaccga gttccagtcc gtgacactca caatgcagag actgctggcc aatcacagca
     1681 acgaactgcc ttctctgtgc atgctcaaca acagcttcta ctacatgagg ggcggcgtga
     1741 acacctttct gatcagagtg tccgacatct ccgtgctcat gaaggagtac gacgtgtcca
     1801 tctacgaacc cgaggatctg ggcaactgcc tcaataaaag cgatagcagc tgggccattc
     1861 actggttcag caatgctctg ggccacgact ggctgatgga tcctcccatg ctgtgtagaa
     1921 acaagaccaa gaaggagggc tccaacatcc agttcaacat cagcaaagcc gacgacgcca
     1981 gagtctacgg caagaaaatt agaaatggca tgaggcatct gttcagaggc ttccacgacc
     2041 cttgcgaaga gggaaaggtc tgctacctca ccattaatca gtgcggcgac cctagcagct
     2101 tcgactactg cggcgtgaac catctgagca agtgccagtt cgaccacgtg aacacactgc
     2161 actttctcgt gaggtccaag acccacctca actttgagag gtctctgaag gccttctttt
     2221 cttggtctct gaccgatagc agcggaaagg acatgcccgg cggctattgc ctcgaggagt
     2281 ggatgctgat cgccgctaaa atgaagtgct tcggcaacac agctgtggcc aaatgcaacc
     2341 agaatcacga ctccgagttt tgcgacatgc tgagactgtt cgactacaac aagaacgcca
     2401 ttaaaacact gaacgatgaa tccaagaagg agatcaatct gctcagccag acagtcaatg
     2461 ctctgatcag cgacaatctg ctgatgaaga acaagatcaa ggaactgatg agcatcccct
     2521 actgtaacta tacaaagttc tggtacgtca atcatacact gaccggccaa cacacactcc
     2581 ctagatgctg gctcatcaga aatggctcct acctcaacac aagcgagttc agaaatgact
     2641 ggattctcga gtccgatcac ctcatcagcg agatgctgtc caaagagtat gccgagaggc
     2701 aaggcaaaac ccccatcaca ctggtcgaca tctgcttttg gagcaccatc ttcttcacag
     2761 cctctctgtt tctgcatctg gtgggcatcc ccacacatag acatctgaag ggagaggctt
     2821 gccctctgcc ccacaagctg gacagcttcg gaggctgtag atgcggcaag taccctaggc
     2881 tgaagaagcc caccatctgg cacaagaggc attgatgtct tcctggagct ccagtcattg
     2941 actataacgt ctcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3001 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3061 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3121 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3181 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3241 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3301 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3361 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ggatcggccg
     3421 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     3481 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     3541 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     3601 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3661 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3721 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3781 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3841 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3901 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3961 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4021 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4081 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4141 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4201 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4261 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4321 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4381 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4441 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4501 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4561 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4621 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4681 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4741 ccacgctcac cggcaccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4801 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4861 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4921 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4981 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5041 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5101 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5161 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5221 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5281 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5341 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5401 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5461 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5521 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5581 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     5641 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     5701 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     5761 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     5821 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     5881 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     5941 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6001 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     6061 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6121 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6181 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc
     6241 tccaccgcgg tg
//
