LOCUS       sequence-407402-        9024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Oropouche virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_OROV_GP
FEATURES             Location/Qualifiers
     source          1..9024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5729..5748)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5794..5849
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5848..5867)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6207..6223)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6207..6223)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6220..6242)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6231..6247
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6255..6261,6262..6279,6280..6285))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6300..6321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6438..6455)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6609..7197)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6689..6708)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7368..8159,8160..8228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7991..8010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8229..8333)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8359..8814)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8496..8517)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8708..8727
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8941..8963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8955..8972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8956..8972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8982..9001
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8982..9000
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggcc aacctcatta
     1441 tcatcagcat ggtgctcggc gtcgcttatg gccatcctct gagcacatcc cagatcggcg
     1501 atagatgttt tgctggcggc aacctcttca aggaaatgaa cctctccgtc ggactgggcg
     1561 aaatctgcgt gaaagacgac atcagcattg tgaaatccac caccgtcttt tccaagaata
     1621 agcccgccct cgaggctaca accaaattct atagaagctt cattgtgaag gattggagcg
     1681 agtgtaatcc cgtgctcgac aaattcggca acttcatggt cctcagcgtg gacgacaacg
     1741 gacacatcat ccctaagatg tacacatgta gggccgcttg tgacatcaga ctcaataagg
     1801 ataacgccga gatcattctg agcagcacca agctgaacca tttcgagatc gtgggcacaa
     1861 ccagcacctc cggatggttt aagaacacaa ttacaaataa tctcgaacac acatgcgagc
     1921 acgtcacagt caactgtgga caaaagtccg tcaagttcca cgcttgtttc agacagcata
     1981 gaggctgcat cagattcttt aagggcacct acatgcccta cagcatgatt gaagccatgt
     2041 gtgtgaatat tgagctcatt attctgacac tgtatatctt cgctgccatc atcttcgccc
     2101 tcattatcac caaatcctac gtcgcctatc tgctgctccc tctgttctac cccgtgacat
     2161 ggttctatgg caaagtctac aaaaagatca acagctgccc taactgtctg ctggcctccc
     2221 accctttcac aagctgcccc aaattttgca tctgtggctc tagattcagc tgcacagagg
     2281 ctctgaaagt gcataggatg ggcaaggact gtctgggcta taagtctctg agcaaggcca
     2341 gacagatgtg taagagcaag tcttggagct ttaccgccgc tattctgacc ggactgattc
     2401 tgatggaatt tatcagcccc attgccggcg agagaatgta caaactcgag gagctggccg
     2461 atgagttcat caagctgaca gaacaagtga acatcctcga gagagagatg gaggtgctca
     2521 agcaatccat catcgtcatg tttgccatca tcctcgtgct gctgctcttc gagaacatga
     2581 tcttcaatag actgtttaga attttttata gaagctgcag catgtgtgga ctgatccact
     2641 atagacccgg actgaaaatc gatctgacaa aaaccaacag atgcggaagc tgcatttgcg
     2701 gctttgatga gcagcagtcc agcggattcg agtatgagat ttttctgaaa gatatgcatg
     2761 tccagaagga gagctgcaaa ttttccccca gagtcaacca ttttagaaac atcaaggctc
     2821 tgctgttcgc tctggtgatt tgtgccacaa tctacacagt gtatgccgac gaggactgtc
     2881 tgtccaagga tatcaaaatt acataccaag agctgcacaa ctgcatcggc cccaagatta
     2941 tgggcgacac atgcgtgtcc aaatccgaac tgtactccga cctcttcagc aagaatctgg
     3001 tgaccgagta cgacaaaaaa tacttcgagc ccgacaccgt caatgaccag ttcaacaaaa
     3061 ttgagttcgc ccaagatgcc cacagaatga tcctcctcga gaggattctg tacaagaccg
     3121 agtgtgaaat gctctctctg aagaaaaaca gcggacccta caacgtggct tggagaacct
     3181 atctcaagaa ccacaacatc gatctgtgta gcagacacaa ctacaagatg atttgccagt
     3241 gcatcaacac acactccatg tgtaataata cagacatcga ttataacaag gaaattgaga
     3301 cctactacaa atccaatgcc gccgcttata gagctgatct gaacaccatc atggataccc
     3361 tcaagaccgc ttttagaggc ctcaccaagg tgctgatcga aaattacatc gagaaagacg
     3421 atagcgatgc cctcaaggct ctgttttcca acattaccga ctccgtgcaa gacaactatc
     3481 agatgatcgg cattctgaag ttcgcttcca agctgctcga catcaacctc ggaagaagca
     3541 caagaagcgc tcatcacagc atcatgacca atgagatccc caaatccaac cctttcaccg
     3601 actactccta tagcaacctc aatattaagg agtgtatgtc ccccgaatcc ctcaaatgct
     3661 tcaaaaagag gggctccaca ccccatacca accatctgct gtgtaagatt gataacaagt
     3721 acaaagcctt cgactggccc gagatcgaga ccatccagaa gggccagaag ctgtgcctcg
     3781 gcgacagcca ctgtaatctc gagttcaccg ccatcacagc cgacaagatt atgagcctca
     3841 ccaattgtta caaagaatcc ttcaccgctc agcccgccga tatgcaagct ggaatcaaaa
     3901 agtgtagcgc cgacgaaatc ggagagtgca ccacactcga ggacaagaca tggcccatca
     3961 ttttctgcgg aggcaagtac tactacagcg atagcaaaga gcacgccaag gatggctcca
     4021 ttaacaacta ctgtctgacc aataaatgta gcgaacaaag gttccccatc cacgaaaact
     4081 ggttcaaaaa gtgcaattgg gacaagaccc acaaagaatt tagcaccatg agacagatta
     4141 actataatga catcaccagc tatagaaagg ccatcgaaag cgaaatcggc accgacctca
     4201 tgacacacca ttacaagccc acaaagaacc tcccccacgt ggtgcctaga taccattcca
     4261 ttgatgtcca aggcaccgag tccaccgagg gcattatcaa tggattcatc caaaacacca
     4321 tccccgccat tagcggactg ggcgtgggct atcatctcaa ctttcagagc aaccagctct
     4381 tcgacattgt gattttcgtg aaaaaagctg tgtataaggc tcaataccaa aaggcctata
     4441 ccaccggacc tagcatcagc atcaatatcg agcataatga gaggtgcacc ggccactgcc
     4501 ccgaaaagat ccccgccaag gaaggctggc tcacatttag caaggagcat accagcagct
     4561 ggggatgcga ggaatatgga tgtctggcca tcgataccgg ctgtctgtac ggcagctgcc
     4621 aagacgtgat tagacccgaa ctggatgtct ataagaagat cggcagcgaa gtgtccctca
     4681 ttgaaatctg catcacactg cctcacgaaa cctactgcaa cgatatggac atcctcgagc
     4741 ctatcattgg agataaactg agcgcctcct ttcagaacac ccaaaccaat cagctgccca
     4801 cactgatcgc ttacaagaag ggaaaaatct ataccggcca gatcaacgac atcggaaaca
     4861 ccgctctgca atgcggatcc atccaagtga ttaacggctc cacaattgga accggcagcc
     4921 ccaagtttga ctatatctgt cacgccatga ggaggaagga cgtcatcgtg agaaagtgtt
     4981 tcaatgataa ttaccagagc tgtacaagac tcgagaagag gaacgacctc atcccctata
     5041 gaaagggcga cgtgatcgag gtgagcaaga ccggatccaa catgggacag atgaccttca
     5101 aaatcgagct cggagacatt aactataaaa ttttcacaaa gtccatcgat ctgcagatga
     5161 gcggcatttg cgctggctgt atcgattgtg ccgaaggcat cagctgcagc atcaatgccg
     5221 aggtccccgc cgagacagtc tgccactgca agaccaattg tgaggatttt attaacaaca
     5281 ttgtcttttc cccccaaatc aagaactaca atatcaaagt gcactgtaag agcaaagtcg
     5341 aaaagattac cgcccacatt tgcggaaggg acatcgatct gcagctgaca atcaagcctt
     5401 ataaccaaaa gatcgatctg agccaactgg acgagagcaa ctatatcaga gaggaggatc
     5461 tgcagtgtgg cacatggctg tgcaaggtgc agaaggaggg catcgacatt atcttcaagg
     5521 gactcttctc cggcctcggc agatactgga caatcctcat ctactccatc atcggcgtgg
     5581 tcatcatcgt gatcctcgtc tacattctgc tgcctattgg aaggctgctg aaggcctttc
     5641 tgattagaca cgagattgaa tacgccatgg agcagaagat taaatgatgt cttcctggag
     5701 ctccagtcat tgactataac gtctcagatc tttttccctc tgccaaaaat tatggggaca
     5761 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     5821 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     5881 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     5941 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     6001 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     6061 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     6121 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     6181 acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     6241 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6301 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6361 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6421 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6481 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6541 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6601 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6661 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6721 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6781 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6841 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6901 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6961 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7021 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7081 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7141 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7201 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7261 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7321 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7381 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7441 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7501 ataccgcgag acccacgctc accggcacca gatttatcag caataaacca gccagccgga
     7561 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7621 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7681 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7741 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7801 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7861 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7921 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7981 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8041 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8101 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8161 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8221 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8281 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8341 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     8401 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     8461 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     8521 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     8581 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     8641 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     8701 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     8761 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     8821 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8881 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8941 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     9001 gcgaattgga gctccaccgc ggtg
//
