LOCUS       sequence-407403-        6582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of env polyprotein from Avian leukosis virus (ALV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6582)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_ALV_Env
FEATURES             Location/Qualifiers
     source          1..6582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3287..3306)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3352..3407
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3406..3425)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3765..3781)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3765..3781)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3778..3800)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3789..3805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3813..3819,3820..3837,3838..3843))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3858..3879
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3996..4013)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4167..4755)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4247..4266)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4926..5717,5718..5786))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5549..5568
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5787..5891)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5917..6372)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6054..6075)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6266..6285
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6499..6521
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6513..6530
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6514..6530
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6540..6559
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6540..6558
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggag gccgtgatta
     1441 aagcctttct gaccggatac cccggaaaga cctccaaaaa ggatagcaag gagaagcctc
     1501 tggccaccag caagaaggac cccgaaaaaa cccctctgct gcccacaaga gtgaactaca
     1561 ttctgatcat cggcgtgctg gtgctgtgcg aggtgaccgg cgtcagagct gacgtccatc
     1621 tgctcgagca gcccggcaac ctctggatta catgggccaa cagaaccggc cagaccgatt
     1681 tttgtctgag cacacagagc gctacctccc cttttcagac atgtctgatc ggcattccta
     1741 gccctattag cgagggagac ttcaagggct acgtgagcga caactgcaca acactgggaa
     1801 cagacagact cgtgagctcc gccgatttca ccggcggacc cgacaactcc accacactga
     1861 cctatagaaa agtcagctgt ctgctgctca agctcaacgt gagcatgtgg gacgaacctc
     1921 acgagctgca gctgctggga tcccaatccc tccctaacat cacaaacatc gcccagatca
     1981 gcggcatcac cggcggatgc gtgggattta gaccccaagg cgtgccttgg tatctcggat
     2041 ggtccagaca agaggccaca aggtttctgc tgagacaccc cagctttagc aagtccaccg
     2101 aacccttcac cgtcgtcacc gccgatagac acaatctgtt catgggctcc gagtactgtg
     2161 gcgcctatgg atatagattc tggaacatgt ataactgcag ccaagtgggc agacaatata
     2221 gatgtggcaa tgccagatcc cctaggcccg gcctccccga gatccagtgt accagaagag
     2281 gcggaaagtg ggtgaaccag tcccaagaga ttaacgagtc cgaacctttc tccttcaccg
     2341 tgaactgcac agctagcagc ctcggcaacg cctccggatg ttgtggaaaa gctggaacca
     2401 ttctgcccgg caagtgggtg gactccacac aaggaagctt caccaagccc aaagctctgc
     2461 cccccgccat tttcctcatc tgtggagata gagcttggca aggcattcct agcagacccg
     2521 tgggcggacc ttgttatctg ggcaagctga caatgctcgc ccccaagcac accgacatcc
     2581 tcaaagtgct ggtgaacagc agcagaaccg gcatcagaag gaagagaagc acatcccacc
     2641 tcgatgacac atgcagcgat gaggtgcaac tgtggggccc caccgccaga atcttcgcca
     2701 gcattctggc ccccggcgtg gctagagctc aagccctcag agagatcgaa agactggctt
     2761 gttggagcgt caaacaagcc aatctgacca catccttcct cggcgatctg ctggacgacg
     2821 tcaccagcat cagacacgcc gtgctgcaaa atagggccgc tatcgacttt ctgctgctgg
     2881 ctcacggcca tggctgtgag gatgtcgccg gcatgtgctg tttcaatctg agcgaccact
     2941 ccgagagcat ccagaagaag ttccagctca tgaaggagca cgtgaacaag atcggagtgg
     3001 atagcgaccc cattggaagc tggctcagag gactgtttgg cggcatcggc gaatgggctg
     3061 tgcatctgct gaagggactg ctgctgggac tcgtggtgat tctgctgctg gtggtgtgcc
     3121 tcccttgtct gctgcagatc gtctgcggaa atattagaaa aatgatcaat aacagcatca
     3181 gctatcacac cgagtacaag aagctgcaga aggcttgcgg ccagcccgag agcagaattg
     3241 tctgatgtct tcctggagct ccagtcattg actataacgt ctcagatctt tttccctctg
     3301 ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga
     3361 aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat
     3421 atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat
     3481 atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga
     3541 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     3601 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     3661 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     3721 tcttatggag atccctcgac ggatcggccg caattcgtaa tcatgtcata gctgtttcct
     3781 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3841 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3901 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3961 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     4021 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     4081 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     4141 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     4201 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     4261 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     4321 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4381 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4441 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4501 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4561 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4621 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4681 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4741 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4801 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4861 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4921 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4981 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5041 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggcaccaga tttatcagca
     5101 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5161 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5221 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5281 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5341 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5401 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5461 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5521 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5581 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5641 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5701 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5761 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5821 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5881 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     5941 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     6001 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     6061 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     6121 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     6181 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     6241 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     6301 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     6361 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     6421 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     6481 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt
     6541 aatacgactc actatagggc gaattggagc tccaccgcgg tg
//
