LOCUS       sequence-407405-        6339 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Australian bat lyssavirus G protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_ABLV_G
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(3044..3063)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3109..3164
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3163..3182)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3522..3538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3522..3538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3535..3557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3546..3562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3570..3576,3577..3594,3595..3600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3615..3636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3753..3770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3924..4512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4004..4023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4683..5474,5475..5543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5306..5325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5544..5648)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5674..6129)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5811..5832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6023..6042
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6256..6278
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6270..6287
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6271..6287
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6297..6316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6297..6315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgctg ctgcaagtga
     1441 ttctgctggt gagcctcacc gctattctgc cttgcaccgg ccagttccct ctgtacgcta
     1501 ttcccgacaa gctcggacct tggagcccca tcgacattca tcatctgagc tgccccaata
     1561 acctcattgt ggaggatgag ggctgcacat ccctctccgg atttagctac atggagctga
     1621 aggtgggctt tatcaccacc atcaaggtct ccggattcac atgcaccgga gtcgtgacag
     1681 aatccgagac ctacacaaac ttctttggct acgtgaccac caccttcaag aggaagcact
     1741 tcagacctac ccccgagagc tgtagaaagg cttacaactg gaagatcgct ggcgacccta
     1801 gatacgaaga atctctgcat aatccctacc ccgattacca ttggctgaga acagtgacca
     1861 ccacaaagga gagcctcctc atcatctccc ccagcgtggt ggatatggat ccctacgaca
     1921 agtctctgca ctctagaatg tttcctaaag gcagctgcag cggcgcttcc atccctagcg
     1981 tgttctgttc caccaaccac gactatacac tgtggatgcc cgaggacagc aactccggca
     2041 tgagctgcga catcttcacc atgtccaagg gcaagaaagc ttccaagggc ggcaaggtgt
     2101 gtggcttcgt ggacgaaaga ggactgtaca agtctctgaa gggcgcttgc aaactgaagc
     2161 tctgcggcat cagcggactg agactgctgg acggcagctg ggtgtccatc cagaaccacg
     2221 aggaggtgaa atggtgtagc cctaaccagc tcgtcaacat ccacgacttt aacgccgacg
     2281 agattgagca cctcatcgtg gaagagctca tcaaggagag agaggagtgc ctcgatgctc
     2341 tcgagtccat catcacaacc aagtctgtga gctttagaag gctgtcccat ctgagaaagc
     2401 tcgtgcccgg attcggcaag gcttacacca tcatcaacaa aacactgatg gaggccgacg
     2461 cccactacaa aagcgtgaga acatgggacg agatcatccc ttccaaggga tgcctcaagg
     2521 tgagggaaaa gtgccacccc ccttacaacg gcgtgttctt caacggcatc atcctcggcc
     2581 ccgacggcca agtcctcatt cccgaaatgc aatcctctct gctgcatcag catacagagc
     2641 tgctcgagtc ctccgtgatc cctctgattc accctctcgc cgatcccagc accatcttta
     2701 gaggagatga cgaggctgag ggctttatcg aagtccatct gcccgacatc caaaagcaag
     2761 tgtccggcat tgatctggga ctgtccgagt gggagaggta tctgatcatt ggcatcagcg
     2821 ccatcattct gttcattctg gccattattt tcacaatctg ctgtagaagg tgcaagagga
     2881 ggaagaagat taggaccgac cacatcgagc tcgatagaaa agtgagcgtg accagccaat
     2941 ccggaaagtc catccccagc tgggagtcct acaaatctag acaaggccat agcagaagct
     3001 gatgtcttcc tggagctcca gtcattgact ataacgtctc agatcttttt ccctctgcca
     3061 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3121 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3181 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3241 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3301 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3361 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3421 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3481 tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg
     3541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4801 cccagtgctg caatgatacc gcgagaccca cgctcaccgg caccagattt atcagcaata
     4861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5641 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5701 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5761 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5821 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5881 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5941 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6001 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6061 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6121 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6181 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6241 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     6301 acgactcact atagggcgaa ttggagctcc accgcggtg
//
