LOCUS       sequence-407406-        6201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Guanarito virus GPC protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6201)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_GTOV_GPC
FEATURES             Location/Qualifiers
     source          1..6201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2906..2925)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2971..3026
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3025..3044)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3384..3400)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3384..3400)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3397..3419)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3408..3424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3432..3438,3439..3456,3457..3462))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3477..3498
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3615..3632)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3786..4374)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3866..3885)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4545..5336,5337..5405))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5168..5187
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5406..5510)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5536..5991)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5673..5694)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5885..5904
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6118..6140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6132..6149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6133..6149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6159..6178
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6159..6177
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga cagctgatct
     1441 ccttcttcca agacattccc attttcttcg aggaggccct caatgtcgcc ctcgctgtgg
     1501 tgacactgct cgccatcatc aagggcatcg tcaacgtgtg gaagagcggc attctgcagc
     1561 tgtttgtgtt cctcgtgctg gctggcagaa gctgctcctt caaagtgggc caccacacca
     1621 atttcgagag cttcaccgtg aagctgggcg gcgtgttcca cgaactgccc agcctctgta
     1681 gggtcaacaa ctcctactct ctgattagac tgagccacaa ctccaatcaa gctctgtccg
     1741 tcgagtatgt ggacgtgcat cccgtcctct gtagcagcag ccccaccatt ctggacaact
     1801 atacccagtg catcaaggga agccccgagt tcgactggat tctgggctgg acaattaagg
     1861 gactgggcca tgacttcctc agagacccta ggatctgctg cgagcccaaa aagaccacca
     1921 acgtcgagtt cacctttcag ctgaatctga cagacagccc cgagacacac cactatagaa
     1981 gcaaaattga ggtgggcatt agacatctgt tcggcaacta catcaccaat gacagctaca
     2041 gcaagatgtc cgtggtgatg aggaacacaa catggaaggg ccagtgccct aacagccatg
     2101 tgaacaccct cagatttctg gtgaagaacg ccggctatct ggtgggaaga aagcctctgg
     2161 ctttcttctc ttggtctctg tccgacccca aaggaaacga catgcccggc ggatattgtc
     2221 tggaaaggtg gatgctcgtg gctggcgatc tcaagtgttt cggcaacacc gccgtggcca
     2281 agtgcaatct gaaccacgac tccgagttct gcgacatgct gagactgttc gatttcaata
     2341 aaaacgccat cgaaaagctc aataaccaga ccaagaccgc tgtgaacatg ctgacccaca
     2401 gcatcaattc tctgatcagc gacaacctcc tcatgaggaa caagctgaag gagatcctca
     2461 aggtgcccta ctgcaactac acaagattct ggtacatcaa ccacaccaag agcggcgagc
     2521 actctctgcc cagatgctgg ctggtgtcca acggctccta tctgaacgag agcgacttta
     2581 gaaatgagtg gatcctcgag tccgaccatc tgatcgctga gatgctctcc aaggagtacc
     2641 aagatagaca aggcaaaacc cctctgacac tggtggatct gtgcttttgg agcgccatct
     2701 tcttcaccac ctctctgttt ctgcatctgg tgggatttcc cacccacaga cacatccaag
     2761 gcggaccttg ccctctgcct catagactgg acagaaatgg agcttgcaga tgcggaagat
     2821 atcagaatct gggcaagcaa gtgacatgga agaggaaaca ctgatgtctt cctggagctc
     2881 cagtcattga ctataacgtc tcagatcttt ttccctctgc caaaaattat ggggacatca
     2941 tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc attgcaatag
     3001 tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttaa
     3061 aacatcagaa tgagtatttg gtttagagtt tggcaacata tgcccatatg ctggctgcca
     3121 tgaacaaagg ttggctataa agaggtcatc agtatatgaa acagccccct gctgtccatt
     3181 ccttattcca tagaaaagcc ttgacttgag gttagatttt ttttatattt tgttttgtgt
     3241 tatttttttc tttaacatcc ctaaaatttt ccttacatgt tttactagcc agatttttcc
     3301 tcctctcctg actactccca gtcatagctg tccctcttct cttatggaga tccctcgacg
     3361 gatcggccgc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc
     3421 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3481 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     3661 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     3721 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3781 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3841 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3901 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3961 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4021 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4081 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4141 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4201 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4261 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     4321 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     4381 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     4441 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4501 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4561 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4621 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     4681 ccgcgagacc cacgctcacc ggcaccagat ttatcagcaa taaaccagcc agccggaagg
     4741 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     4801 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     4861 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     4921 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     4981 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5041 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5101 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5161 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5221 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5281 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5341 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5401 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     5461 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     5521 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     5581 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     5641 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     5701 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     5761 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     5821 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     5881 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     5941 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     6001 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     6061 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     6121 agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg
     6181 aattggagct ccaccgcggt g
//
