LOCUS       sequence-407407-        8355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Rift valley fever virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_RVFV_GP
FEATURES             Location/Qualifiers
     source          1..8355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5060..5079)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5125..5180
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5179..5198)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5538..5554)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5538..5554)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5551..5573)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5562..5578
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5586..5592,5593..5610,5611..5616))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5631..5652
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5769..5786)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5940..6528)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6020..6039)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6699..7490,7491..7559))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7322..7341
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7560..7664)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7690..8145)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7827..7848)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8039..8058
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8272..8294
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8286..8303
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8287..8303
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8313..8332
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8313..8331
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgtac gtgctgctca
     1441 ccattctcat cagcgtgctc gtgtgcgagg ccgtgatcag agtgtctctg agcagcacaa
     1501 gggaggagac atgcttcggc gattccacca accccgagat gatcgaggga gcttgggact
     1561 ctctgaggga ggaggagatg cccgaggagc tcagctgctc cattagcggc attagagagg
     1621 tcaaaacctc ctcccaagag ctctacagag ctctgaaggc catcattgcc gctgacggac
     1681 tcaacaacat cacatgccac ggcaaggacc ccgaggacaa aatcagcctc atcaagggac
     1741 ccccccataa aaaaagagtc ggcatcgtga gatgtgaaag gaggagggac gccaagcaga
     1801 ttggcagaga gaccatggcc ggaattgcca tgacagtcct ccccgccctc gccgtgttcg
     1861 ccctcgctcc cgtggtgttc gccgaggatc cccatctgag aaataggccc ggcaagggac
     1921 acaactacat cgatggcatg acccaagagg atgctacatg caagcccgtg acatacgccg
     1981 gagcttgttc cagcttcgac gtgctgctgg aaaagggaaa gttccctctg tttcagtcct
     2041 acgcccatca tagaacactg ctcgaagctg tccacgacac cattatcgcc aaggctgacc
     2101 ctcccagctg cgatctgctg agcgctcatg gaaacccttg catgaaagag aagctcgtga
     2161 tgaaaaccca ctgccctaac gactaccagt ccgcccacta cctcaacaat gacggcaaga
     2221 tggcttccgt caaatgccct cccaaatatg agctgacaga ggactgcaac ttttgtagac
     2281 aaatgaccgg agccagcctc aagaagggca gctatcctct ccaagatttg ttctgccaga
     2341 gctccgagga cgacggctcc aagctcaaga caaaaatgaa gggagtgtgt gaagtgggag
     2401 tgcaagctct caaaaagtgt gacggccaac tgtccaccgc ccatgaagtg gtccccttcg
     2461 ccgtgttcaa gaacagcaag aaggtctatc tggacaagct ggacctcaag accgaggaga
     2521 atctgctgcc cgatagcttc gtgtgcttcg agcataaagg ccaatacaaa ggcaccatgg
     2581 actccggaca gacaaagagg gagctcaaaa gcttcgatat tagccagtgc cctaagatcg
     2641 gcggccatgg cagcaaaaaa tgcaccggcg atgccgcttt ctgctccgct tacgagtgca
     2701 cagcccaata cgctaacgct tactgcagcc acgctaacgg cagcggaatc gtgcagattc
     2761 aagtgtccgg cgtctggaaa aagcctctgt gtgtgggata cgagagggtc gtcgtgaaga
     2821 gggagctgag cgccaaacct attcagaggg tggaaccttg cacaacatgc attaccaagt
     2881 gcgagcccca cggactggtg gtgaggagca ccggcttcaa aatcagcagc gctgtggctt
     2941 gcgcttccgg agtctgtgtg accggctccc agtcccccag caccgagatt accctcaaat
     3001 atcccggcat ctcccagtcc agcggcggcg atattggagt gcatatggcc cacgacgatc
     3061 agtccgtcag ctccaagatt gtcgcccact gtccccctca agacccttgt ctggtgcacg
     3121 actgtattgt gtgcgctcac ggactgatca actaccagtg ccataccgct ctgagcgcct
     3181 tcgtggtcgt gttcgtgttc tcctccatcg ctatcatctg tctggccatt ctctataggg
     3241 tcctcaaatg tctgaaaatc gcccctagaa aggtgctcaa tcccctcatg tggattaccg
     3301 ccttcattag atggatctat aagaaaatgg tggccagagt ggccgacaac attaaccaag
     3361 tgaatagaga aatcggctgg atggagggcg gacagctcgt gctgggcaat cccgccccta
     3421 ttcctagaca cgcccccatt cccagatact ccacctatct gatgctcctc ctcattgtgt
     3481 cctacgctag cgcttgcagc gaactgatcc aagccagctc tagaattacc acatgcagca
     3541 cagaaggagt gaataccaag tgcagactga gcggcaccgc tctcattagg gctggcagcg
     3601 tgggcgctga ggcttgcctc atgctcaagg gagtgaagga ggatcaaacc aagtttctga
     3661 agatcaagac cgtcagctcc gagctgagct gtagagaagg ccagtcctat tggaccggaa
     3721 gctttagccc caagtgtctg tcctctagaa gatgtcatct ggtcggagaa tgtcacgtga
     3781 atagatgtct gtcttggaga gataacgaga ccagcgccga atttagcttc gtgggcgagt
     3841 ccaccaccat gagggagaac aagtgcttcg aacagtgcgg aggctggggc tgtggctgct
     3901 tcaacgtgaa tcctagctgt ctgtttgtgc acacctatct gcagtccgtg aggaaggaag
     3961 ctctgagagt ctttaactgc atcgactggg tgcataagct cacactcgag attaccgact
     4021 tcgatggatc tgtgagcacc atcgatctgg gcgccagcag cagcagattc acaaattggg
     4081 gctctgtgtc tctgtctctg gatgccgaag gcatttccgg atccaacagc ttctccttca
     4141 tcgaatcccc cggcaaggga tacgccattg tggatgagcc cttcagcgag attcctagac
     4201 aaggctttct gggcgagatc agatgtaaca gcgagtccag cgtgctgtcc gctcacgaga
     4261 gctgtctgag ggcccctaac ctcatctcct acaaacccat gattgaccag ctcgagtgca
     4321 ccaccaatct gatcgacccc ttcgtggtgt tcgagagggg ctctctgcct caaacaagaa
     4381 acgacaaaac attcgccgct agcaagggca atagaggagt gcaagccttt agcaagggca
     4441 gcgtccaagc cgacctcacc ctcatgttcg acaattttga ggtggacttc gtgggcgctg
     4501 ctgtgagctg cgatgctgct ttcctcaatc tgaccggctg ctatagctgc aatgccggcg
     4561 ccagagtctg tctgagcatt acctccaccg gcaccggctc tctgtccgct cataacaaag
     4621 acggatctct gcacatcgtg ctcccttccg aaaatggcac aaaggaccag tgccagattc
     4681 tccactttac cgtgcccgag gtcgaggagg agttcatgta cagctgcgac ggcgacgaga
     4741 ggcctctcct cgtcaagggc accctcatcg ctatcgaccc ctttgacgac agaagagagg
     4801 ccggcggcga aagcacagtg gtcaacccca aaagcggaag ctggaacttc tttgactggt
     4861 tctccggact gatgagctgg tttggcggcc ctctgaaaac aattctgctc atctgtctgt
     4921 acgtggctct cagcatcggc ctcttctttc tgctgatcta tctcggaaga accggcctca
     4981 gcaagatgtg gctggctgct acaaagaagg ctagctgatg tcttcctgga gctccagtca
     5041 ttgactataa cgtctcagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc
     5101 cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt
     5161 ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc
     5221 agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca
     5281 aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     5341 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     5401 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     5461 cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc gacggatcgg
     5521 ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5581 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5641 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5701 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5761 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5821 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5881 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5941 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     6001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     6061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     6121 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     6181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6241 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6301 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6361 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6421 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6481 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6541 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6601 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6661 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6721 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6781 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6841 gacccacgct caccggcacc agatttatca gcaataaacc agccagccgg aagggccgag
     6901 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6961 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7021 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7081 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7141 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7201 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7261 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7321 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7381 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7441 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7501 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7561 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7621 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7681 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7741 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7801 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7861 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7921 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7981 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8041 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8101 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     8161 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     8221 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     8281 gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg
     8341 agctccaccg cggtg
//
