LOCUS       sequence-407408-        8703 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Punta toro phlebovirus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_PTV_GP
FEATURES             Location/Qualifiers
     source          1..8703
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5408..5427)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5473..5528
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5527..5546)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5886..5902)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5886..5902)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5899..5921)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5910..5926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5934..5940,5941..5958,5959..5964))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5979..6000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6117..6134)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6288..6876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6368..6387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7047..7838,7839..7907))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7670..7689
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7908..8012)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8038..8493)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8175..8196)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8387..8406
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8620..8642
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8634..8651
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8635..8651
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8661..8680
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8661..8679
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgatc tttaccatcc
     1441 tcaatgtgct cacaagagcc atgctggtca tgagcatgta ctctctgaca acatgggaca
     1501 gctctagcag aaacgatatc tgcttctcca acgactcccc tctggaaggc ctcgtgtatt
     1561 actgggagac ccacagcaag aagcatgact acaagaagca aggatcccaa aagtgcagag
     1621 tgggcgacag cgataaaaag atgatcagca acgtgacaat catttccctc atcagcgaga
     1681 tccaaaagag catctccgag ctgtctctga gctgcggcaa taacgacaac agcaccggcc
     1741 aagtgctcac cttcaacgga ctcgaggaca caattagagg cgattacatc gtggattgtg
     1801 tgaccggact gtaccagagc gatattggag tgggagtggg actgggaaga attaatcacg
     1861 atcaccaaca agtgaagaac aaagccgtgg tgattgatga gaaggaaaaa atgatttctc
     1921 tgctcgagac ccagcagagc gagaatgaca tcaaaaccca agtgctcatg tccgagatcg
     1981 agcagctcaa aaatcagctg tccaaaagaa gaaacgaaag gggccaagag aagagggacg
     2041 ctgagaaact gatgtccgat ctgatggcca gaaactccga tctgagaaat cacaacgtca
     2101 tcctcaccga cgagattagc cagatcaaga ataacattac cattcataga aatagaaata
     2161 tgatgtccac cacagtcgtg cccgccattc tgtccgtggc tctgctgagc tcctccgtgg
     2221 gccccattac agctgctccc cccgactcca ccatgattaa tccttggcct cacgccaaga
     2281 acagagtcgg caccggcatg tataagtatg atgagaacga cgaatccggc tgcagaccca
     2341 ttaggtatgg agtgagctgc atcggcttcg acttcatgct caagatggat aagtacccct
     2401 tcttcaatgc ttttatcggc cataagacac ccctcgagtc cttcgccgat aaaatcattg
     2461 aaaaagagga agagacatgc gagatcggaa ccaacaagga gttcaaatgc tttgaagaga
     2521 gggcttatat caaaggcaca tgccccacca atatcaatgc cgtgcactac attgacaaca
     2581 agggaaagct gagatacgtg aagtgtaagg agaacctcga gatgaccgag gattgtgctt
     2641 tctgtaggaa gattaagaag aaggctggac agagcgtgca agtgcaaaag accagcgtgc
     2701 ctctgcaaga cgccatttgt caagagaaca gcgatatcta cagcggcccc aaaattccct
     2761 tcaaaggcgt ctgtaagatc ggcctcatta aatataagga gtgcaaattc aaaacctcct
     2821 cctacgagac catctccttt attacactga agggcaaggg aaaaatttat attgagcatc
     2881 tgatgctcaa aaacatcgag gtcgtgacca acgtgtcctt cgtgtgctac gagcacgccg
     2941 gccaagacga gcaagaggtg gagcacagag ctctgaagag ggtgtccgtc aacgactgca
     3001 agatcgtgga caatagcaaa caaaaaatct gcaccggaga ccacatcttc tgcgagaagt
     3061 acgactgctc cacctcctat cccgacgtga catgcattca tacccccggc agcggccctc
     3121 tgtacattaa tctgatgggc agctggatca agccccagtg tgtgggctat gagagggtgc
     3181 tcgtcgacag agaggtcaag caacctctgc tggtgcccga gcagaattgc gatacatgcg
     3241 tgtccgagtg tctggatgaa ggcgtgcaca tcaagagcac cggattcgag atcaccagcg
     3301 ccgtggcttg ctcccatggc agctgcattt ccgcccatca agaggcctcc accagcgtca
     3361 tcatccccta tcccggcctc ctcgcctccg tgggcggcag aattggaatc catctgagcc
     3421 acacaagcga ttccgcctcc gtgcacatgg tcgtggtgtg tccccctagg gatagctgcg
     3481 ccgctcacaa ttgtctgctg tgttaccacg gcattctgaa ctaccagtgc cactccacac
     3541 tgagcgccat tctgacctcc tttctgctga ttctcttcat ctacacagcc ttctccgtga
     3601 caacaaatgt gctctacgtg ctgagactga tccctaaaca actcaagagc cccgtgggct
     3661 ggctcaagct gtttgtgaac tggctgctga cagctctgag gatcaagaca agaagagtca
     3721 tgggcagaat caaccaaagg attggctggg tcgaccatca cgatgtggaa agacctagac
     3781 atagagagcc tatgagaagg ttcaagacca cactgctgct gaccctcatt atggtgaccg
     3841 gcggaaacgc ttgctccaat acagtggtgg ctaacagcaa gcagaccaga tgtgtgcaag
     3901 agggctccaa taccaagtgt agcattaccg ccaccatcac cctcagagcc ggagtcatcg
     3961 gcgccgagag ctgctttatt atcaagggcc ccatggagaa ccagcaaaag accatttcca
     4021 ttaaaaccat ttcctccgaa accgtgtgca gagagggaag ctccttctgg acaagcctct
     4081 acatccccag ctgtctctct agcagaaggt gccatctggt cggcgattgc gtgggcaata
     4141 agtgtcaatc ttggagggac gaccagctgt ctagagagtt ttccggagtc aaggacaacc
     4201 acattatgaa cgagaacaag tgtttcgaac aatgcggcgc catcggatgt ggatgcttca
     4261 acatcaatcc cagctgtctg tacgtgcatg cctacctcaa gagcgctagg aacgaggccg
     4321 tgagagtgtt cagctgctcc gattgggtcc atagagtgag ctttgaggtg aagggacccg
     4381 acggagaaat ggaactcgtg acactgggaa gcctcggaac aaagttcctc aactggggaa
     4441 cactctccct ctctctggat gccgaaggaa tcagcggcac caactccatc agctttctcg
     4501 agagctccaa gggaggcttt gccctctacg atgagggcta cagcgaaatc cccagagagg
     4561 gcttcctcgg cgaaatcaga tgctcctccg agtccgccgc tatcagcgcc cacaaaagct
     4621 gcattagggc ccccggactg atcaagtaca agcccatgac cgatcagatt gaatgcaccg
     4681 cttctctggt ggaccctttt gctatctttc tgaagggctc tctgccccag acaagaaacg
     4741 gccagacatt caccagcacc aaggacaaaa agaccgtcca agcctttaca aacggcgcca
     4801 tcaaagctct gctgtccatc aatctggacg accacgagat catctttatt aataaggtga
     4861 aaagctgcga cgccaccttc ctcaatgtgt ccggatgcta cagctgcgac tatggagctc
     4921 acgtgtgtgt gaaggtgaag agctccgaat ccgctgattt tttcgccgag tccgaggaca
     4981 aaaccaccgt gctgtccttc cccattcaat ccggcacaag ggattactgc caagtgctcc
     5041 acttccagaa acctctggtc gacgagagac tgagctatag ctgcggcagc gaacccaagc
     5101 tcatcgtcat taagggcaca ctggtgtata tgggcgtgta tgactttaga aacaagaccg
     5161 gaggaagcag cacagtgatc aaccctagcg agggaacatg gagcatcagc aattggtttt
     5221 ccggactcct cgactggctg ggcggaccca tgaaggctat cctcaaaatt ctgggattca
     5281 tcgccattgg cattgtctgc ttcattctgt tcatgattct cattagaatc gccgtgaata
     5341 gcatcaacat caaaaaaaaa aattgatgtc ttcctggagc tccagtcatt gactataacg
     5401 tctcagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5461 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     5521 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     5581 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     5641 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     5701 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     5761 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     5821 cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc gcaattcgta
     5881 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5941 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6001 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6061 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6121 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6181 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6241 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6901 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6961 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7021 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7081 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7141 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7201 ccggcaccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7261 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7321 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7381 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7441 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7501 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7561 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7621 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7681 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7741 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7801 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7861 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7921 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7981 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     8041 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8101 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8161 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8221 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8281 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8341 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8401 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8461 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     8521 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     8581 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     8641 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctccaccgcg
     8701 gtg
//
