LOCUS       sequence-407410-        8823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of MERS coronavirus S protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8823)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_MERS-CoV_S
FEATURES             Location/Qualifiers
     source          1..8823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5528..5547)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5593..5648
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5647..5666)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6006..6022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6006..6022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6019..6041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6030..6046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6054..6060,6061..6078,6079..6084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6237..6254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6408..6996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6488..6507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7167..7958,7959..8027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7790..7809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8028..8132)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8158..8613)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8295..8316)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8507..8526
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8740..8762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8754..8771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8755..8771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8781..8800
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8781..8799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgatt cactccgtgt
     1441 tcctcctcat gtttctgctc acacccaccg agtcctatgt cgacgtcgga cccgattccg
     1501 tgaaaagcgc ttgcatcgag gtcgacatcc agcaaacatt cttcgacaag acatggccta
     1561 gacccatcga cgtcagcaag gccgatggca ttatctaccc tcaaggaaga acctactcca
     1621 atatcaccat cacctaccaa ggactgttcc cttaccaagg cgaccatggc gatatgtacg
     1681 tctactccgc tggacatgct accggaacaa cacctcagaa gctcttcgtg gccaattact
     1741 cccaagacgt gaagcaattc gccaacggat tcgtggtgag aatcggagcc gccgccaatt
     1801 ccaccggcac agtgatcatt tcccctagca cctccgctac aattagaaaa atctaccccg
     1861 ccttcatgct cggcagctcc gtcggcaact tttccgacgg caagatggga agatttttca
     1921 atcacacact ggtgctgctg cccgatggct gtggcacact gctgagggct ttctattgta
     1981 ttctggaacc tagatccgga aaccattgcc ccgccggcaa cagctacacc tccttcgcca
     2041 catatcacac ccccgctacc gattgcagcg atggcaacta caatagaaac gcctctctga
     2101 actcctttaa agagtacttc aatctcagaa attgcacatt catgtacaca tataacatca
     2161 cagaggacga aatcctcgag tggtttggaa ttacccagac cgctcaaggc gtgcatctgt
     2221 tcagcagcag atatgtcgat ctgtacggcg gaaacatgtt ccagttcgct acactccccg
     2281 tctacgacac catcaaatac tatagcatta ttccccatag catcagatcc atccagagcg
     2341 atagaaaggc ttgggctgcc ttttatgtgt acaagctgca gcctctgaca tttctgctgg
     2401 attttagcgt ggacggctac attagaagag ccatcgattg tggcttcaac gatctgagcc
     2461 aactccattg tagctacgag agcttcgacg tcgaatccgg cgtgtactcc gtcagctcct
     2521 tcgaggccaa acccagcgga tccgtcgtgg agcaagccga aggcgtggaa tgtgatttca
     2581 gccctctgct gagcggcacc cccccccaag tgtacaattt caagaggctg gtgttcacca
     2641 actgtaatta caatctgacc aagctgctgt ctctgtttag cgtcaatgac ttcacatgct
     2701 cccaaatcag ccccgccgcc attgctagca attgttacag ctctctgatc ctcgactatt
     2761 tcagctaccc tctgagcatg aagtccgacc tcagcgtcag cagcgccggc cctatctccc
     2821 agttcaacta taaacagagc ttctccaacc ccacatgcct catcctcgcc accgtccctc
     2881 acaatctgac caccatcaca aagcctctga agtactccta tattaataaa tgcagcagac
     2941 tgctgagcga tgatagaaca gaggtccctc agctggtgaa tgctaatcag tactcccctt
     3001 gcgtgtccat tgtcccctcc acagtgtggg aagatggcga ctactataga aaacagctga
     3061 gccctctgga aggaggaggc tggctggtgg ctagcggcag cacagtcgcc atgacagagc
     3121 aactccaaat gggcttcggc atcacagtgc aatacggaac cgacaccaat tccgtctgcc
     3181 ccaaactcga gttcgctaac gacacaaaga tcgccagcca gctcggaaat tgcgtcgaat
     3241 acagcctcta cggcgtgagc ggaaggggcg tgttccagaa ctgtaccgct gtcggcgtga
     3301 gacaacagag gttcgtctac gacgcctacc agaatctcgt gggctactat tccgatgacg
     3361 gcaactatta ctgtctgaga gcttgtgtga gcgtgcccgt gagcgtgatt tacgacaaag
     3421 agaccaaaac ccatgccaca ctgttcggaa gcgtggcttg cgaacatatt agcagcacca
     3481 tgagccagta tagcagaagc acaagatcca tgctgaagag aagagattcc acatatggcc
     3541 ctctgcagac acccgtcggc tgtgtcctcg gactggtcaa ttcctccctc tttgtcgagg
     3601 attgcaagct ccctctggga cagtctctgt gtgctctgcc cgacacacct tccacactga
     3661 cccctagatc cgtgagaagc gtccccggcg aaatgaggct ggcctccatt gcttttaacc
     3721 accctatcca agtggatcaa ctcaatagca gctacttcaa actgtccatc cccaccaatt
     3781 tctcctttgg cgtcacccaa gagtacatcc aaaccaccat ccagaaggtg accgtggatt
     3841 gcaaacagta cgtgtgcaac ggattccaga aatgcgagca gctgctgaga gagtatggac
     3901 agttctgctc caagatcaat caagctctgc atggcgccaa tctgaggcaa gacgactccg
     3961 tgagaaatct gtttgccagc gtgaagagca gccagagctc ccctatcatc cccggctttg
     4021 gaggagattt caacctcaca ctgctggaac ccgtgagcat ctccaccgga tctagaagcg
     4081 ctagatccgc tatcgaggat ctgctgttcg acaaggtgac catcgccgac cccggataca
     4141 tgcaaggcta tgacgactgc atgcagcaag gccccgcttc cgctagggat ctgatttgcg
     4201 ctcagtatgt ggctggctat aaagtgctgc cccctctgat ggacgtgaat atggaggctg
     4261 cctacacaag ctctctgctc ggctccatcg ccggcgtggg ctggacagcc ggactgtcct
     4321 cctttgccgc tatccccttt gctcaaagca tcttctacag actcaacggc gtgggaatta
     4381 cacagcaagt gctgagcgag aatcagaaac tcatcgctaa caaatttaac caagctctgg
     4441 gcgccatgca gaccggattt acaaccacaa acgaagcctt ccagaaggtg caagacgctg
     4501 tcaacaacaa tgcccaagcc ctcagcaaac tggccagcga actgagcaat acctttggag
     4561 ccatcagcgc cagcattggc gatatcatcc agaggctcga cgtgctggaa caagatgccc
     4621 agatcgacag actcatcaac ggaaggctca caacactcaa cgcctttgtg gcccagcagc
     4681 tggtcagatc cgaatccgcc gctctgtccg ctcaactcgc taaggacaag gtcaacgaat
     4741 gcgtcaaggc ccagagcaag aggagcggct tttgcggcca aggcacccac attgtgagct
     4801 tcgtggtcaa cgctcccaac ggactctact tcatgcacgt cggatactac cccagcaatc
     4861 acattgaggt ggtgtccgcc tacggactgt gtgatgctgc caaccccaca aactgcatcg
     4921 ctcccgtcaa cggctacttt attaagacca acaacacaag gattgtggac gagtggagct
     4981 acaccggaag ctccttctac gctcccgagc ccatcacctc tctgaatacc aagtatgtcg
     5041 ccccccaagt gacctaccag aacattagca ccaatctccc cccccctctg ctgggaaaca
     5101 gcaccggaat tgatttccaa gacgagctcg atgagttctt taaaaacgtg agcacctcca
     5161 ttcctaactt cggatctctg acccagatca ataccacact gctcgatctc acctacgaga
     5221 tgctgtctct gcagcaagtg gtgaaagctc tcaacgagtc ctacatcgac ctcaaggagc
     5281 tgggcaacta cacatattac aacaagtggc cttggtacat ctggctgggc tttatcgccg
     5341 gactggtcgc tctcgccctc tgcgtgttct ttattctgtg ctgtaccggc tgcggaacca
     5401 actgcatggg caagctcaag tgtaacagat gctgcgatag atatgaggaa tacgacctcg
     5461 agcctcacaa ggtccacgtg cactgatgtc ttcctggagc tccagtcatt gactataacg
     5521 tctcagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5581 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     5641 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     5701 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     5761 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     5821 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     5881 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     5941 cagtcatagc tgtccctctt ctcttatgga gatccctcga cggatcggcc gcaattcgta
     6001 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6061 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6121 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     6181 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6241 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6301 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6361 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6421 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6481 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6541 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6601 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6661 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6721 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6781 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6841 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6901 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6961 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7021 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7081 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7141 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7201 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7261 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7321 ccggcaccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7381 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7441 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7501 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7561 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7621 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7681 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7741 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7801 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7861 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7921 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7981 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8041 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8101 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     8161 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8221 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8281 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8341 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8401 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8461 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8521 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8581 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     8641 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     8701 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     8761 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctccaccgcg
     8821 gtg
//
