LOCUS       sequence-407412-        8184 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Blue River virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_BRV_GP
FEATURES             Location/Qualifiers
     source          1..8184
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4889..4908)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4954..5009
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5008..5027)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5367..5383)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5367..5383)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5380..5402)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5391..5407
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5415..5421,5422..5439,5440..5445))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5460..5481
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5598..5615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5769..6357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5849..5868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6528..7319,7320..7388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7151..7170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7389..7493)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7519..7974)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7656..7677)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7868..7887
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8101..8123
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8115..8132
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8116..8132
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8142..8161
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8142..8160
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggtg ggctgggtgt
     1441 gcatctttct ggtggtgctc acaaccgcta ccgccggact caccagaaat ctgtacgagc
     1501 tgaaaattga gtgcccccat accgtgggac tgggacaagg ctacgtgacc ggcagcgtcg
     1561 agacaacacc cgtccctctc ggacaagtga ccgatctgaa gatcgagagc agctgcaatt
     1621 ttgatctgca cgtgcccagc accacaatcc agaagtacaa ccaagtggag tgggccaaaa
     1681 agacctccac caccgagagc acaaatgccg gcgctagcac attcgaggcc aaaaccaagg
     1741 aagtcaatct gaagggcaca tgcaatattc ccgtgaccac cttcgaagct gcctacaagt
     1801 ccagaaagac cgtgatctgc tacgatctgg cttgcaacca gacacactgt ctccctaccg
     1861 tccatctgat tgcccccgtg caaacatgca tgtccgtgag aagctgcatg atcggactgc
     1921 tcagcagcag aatccacgtc atttatgaaa agacctattg tgtgaccggc caagtggtgg
     1981 aaggactgtg ctttatccct acccacacaa ttgctctgac ccaacccggc cacacctacg
     2041 acaccatgac actgcccatc acatgctttc tggtggctaa gaaactggga acccagctga
     2101 agattgccgt ggagctcgag aagctcatca ccgctagcgg ctgcacagag aacagctttc
     2161 aaggctacta catctgcttt ctgggaaagc actccgagcc tctgtatgtc cccaccatgg
     2221 acgactatag gagcgctgag ctcttcacaa gaatggtgct caaccccaag ggagaggatc
     2281 atgaccccga ccaagacggc caaggactga tgagaatcac cggccccatc accgccaagg
     2341 tgccttccac agaaacaacc gagacaatgc aaggcgtggc ttttgctggc acccccatgt
     2401 actcctcctt cagcacactg ctcagaaagg ctgatcccga gtacgtgttc tcccccggca
     2461 ttatccccga gtccaatcac tccgtgtgcg ataagaaaac catgcccatc acatggaccg
     2521 gatttctggc cgtgtccggc gagatcgaga agattaccgg ctgcaccgtg ttctgcacac
     2581 tggccggccc cggcgccagc tgcgaagctt acagcgagac cggcatcttc aatatcagct
     2641 cccctacatg tctggtgaac aaagtgcaaa agtttagagg ctccgagcag agggtcaact
     2701 ttatgtgcca gagggtggac caagacgtcg tcgtgtactg caatggccag aagaaggtga
     2761 ttctgaccaa gacactcgtg attggccaat gcatttacac attcacatct ctgttctctc
     2821 tgctgcccgg agtggctcat agcctcgccg tcgagctctg cgtccccggc ctccatggct
     2881 gggccaccac cgctctgctc atcaccttct gtttcggctg gctgctgatc cctagcgtga
     2941 cactgatcat tctgaggatt ctgagactgc tgacctttag ctgcagccat tactccacag
     3001 agtccaagtt taagattctg ctcgagaagg tgaaagtgga gtatcagaaa acaatgggat
     3061 ccatggtctg cgacatctgt caccatgagt gtgaaaccgc caaggaactc gacacacata
     3121 ggaagagctg ccccgagggc caatgcccct attgcatgac catgacagaa tccaccgaat
     3181 ccgctctgca agctcacttc agcgtctgca aactcacaaa tagattccaa gagaatctga
     3241 aaaagtctct gaagcacccc gaggtgaagc aaggctgcta tagaaccctc ggcgtgttta
     3301 ggtacaagtc tagatgctat gtcggactcg tgtggggcat tctgctgacc gctgagctga
     3361 tcatctgggc tgcctccgct gagacacctc tgatggaaag cggatggagc gatacagccc
     3421 acggcgtggg aattgtccct atgaaaaccg acctcgagct ggatttcgcc ctcgcttcca
     3481 gcagctccta cagctataga aggaagctcg tcaatcccgc caacagcgaa gagacactgc
     3541 ccttccatct gcagctggaa aaacaagtgg tccatgccga gatccataat ctgggccact
     3601 ggatggatgg caccttcaac atcaaaaccg ctttccattg ctatggcgag tgcaagaagt
     3661 acgcttaccc ttggcagaca gctaaatgct tctttgaaaa agactaccag tacgaaacca
     3721 gctggggatg taaccctccc gattgccccg gcgtgggaac cggatggacc gcttgcggag
     3781 tctatctgga caaactgaga agcgtcggca aggcctacaa gattgtgtct ctgaaataca
     3841 caagaaaggt ctgcatccag ctgggaaccg agcagacatg caaacatatc gacgtgaacg
     3901 actgcctcgt cacccccagc gtgaaagtct gtatgatcgg cacagtcagc aagctgcaac
     3961 ccggagacac actgctgttt ctcggccctc tcgagcaagg aggcatcatc ctcaagcaat
     4021 ggtgtaccac cagctgcgtg ttcggagacc ccggcgacat tatgagcacc accagcggaa
     4081 tgaagtgtcc cgagcatacc ggatccttta gaaaaatctg cggattcgcc acaaccccca
     4141 catgcgagta tcaaggcaac acagtgagcg gattcaaaag aatgatggcc acaagagatt
     4201 ccttccaatc cttcaatgtg accgagcccc atattaccag caataggctc gagtggatcg
     4261 accccgatag cagcatcaaa gaccacatca acgtcgtgct gaatagggac gtgtcctttc
     4321 aagacctctc cgataaccct tgcaaagtgg atctccacac ccagtccatc gatggcgctt
     4381 ggggaagcgg cgtcggcttc acactcgtgt gtacagtcgg cctcaccgag tgcgccaatt
     4441 tcattacatc catcaaggct tgcgatctgg ctatgtgcta tggcgccacc gtcaccaatc
     4501 tgctgagagg ctccaacacc gtgaaggtcg tgggcaaggg aggccacagc ggctctctgt
     4561 tcaaatgctg ccacgatacc gattgcaccg acgaaggact ggctgctagc cctcctcatc
     4621 tcgatagggt gaccggattc aatcagatcg acagcgacaa agtgtatgat gacggcgccc
     4681 ccccttgtag cgtcaagtgc tggttcacca agtccggcga gtggctgctg aggattctga
     4741 acggaaactg gatggtggtc gccgtgctga tcgtgatcct catcctcagc attctgctgt
     4801 tctccttctt ctgccctatt agaagcagaa agagcaaacc caattgatgt cttcctggag
     4861 ctccagtcat tgactataac gtctcagatc tttttccctc tgccaaaaat tatggggaca
     4921 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     4981 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     5041 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     5101 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     5161 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     5221 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     5281 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     5341 acggatcggc cgcaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     5401 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5461 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5521 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6121 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6181 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6241 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6301 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6361 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6421 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6481 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6541 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6601 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6661 ataccgcgag acccacgctc accggcacca gatttatcag caataaacca gccagccgga
     6721 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6781 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6841 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6901 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6961 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7021 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7081 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7141 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7201 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7261 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7321 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7381 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7441 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7501 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     7561 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7621 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7681 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7741 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7801 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     7861 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     7921 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     7981 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     8041 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     8101 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     8161 gcgaattgga gctccaccgc ggtg
//
