LOCUS       sequence-407422-        6792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Ebola virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_EBOV_GP
FEATURES             Location/Qualifiers
     source          1..6792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3497..3516)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3562..3617
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3616..3635)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3975..3991)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3975..3991)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3988..4010)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3999..4015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4023..4029,4030..4047,4048..4053))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4068..4089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4206..4223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4377..4965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4457..4476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5136..5927,5928..5996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5759..5778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5997..6101)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6127..6582)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6264..6285)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6476..6495
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6709..6731
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6723..6740
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6724..6740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6750..6769
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6750..6768
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga gtgaccggca
     1441 ttctgcagct ccctagggat agattcaaga ggaccagctt ctttctgtgg gtcattatcc
     1501 tcttccagag gaccttttcc atccccctcg gagtgatcca taacagcaca ctgcaagtca
     1561 gcgacgtgga caaactcgtc tgtagagata agctcagcag cacaaaccaa ctgaggagcg
     1621 tgggactgaa tctcgagggc aatggagtgg ctacagacgt gccttccgct acaaaaaggt
     1681 ggggctttag gagcggcgtg ccccccaaag tggtgaacta tgaggccgga gaatgggccg
     1741 agaattgcta caacctcgag atcaagaaac ccgacggcag cgagtgcctc cccgccgctc
     1801 ccgacggcat taggggattc cctagatgca gatacgtcca taaggtgtcc ggaaccggcc
     1861 cttgcgctgg cgactttgct ttccacaagg aaggcgcctt ctttctgtac gacagactgg
     1921 cctccaccgt gatctataga ggcacaacct tcgctgaggg agtcgtggct tttctcatcc
     1981 tcccccaagc caagaaagac ttcttcagca gccaccccct cagagagccc gtgaacgcta
     2041 ccgaggaccc ttccagcggc tactatagca caaccatcag atatcaagcc accggcttcg
     2101 gaaccaacga aaccgagtat ctgttcgagg tggacaacct cacctatgtg cagctcgagt
     2161 ctagattcac acctcagttt ctgctgcagc tcaacgagac catttacacc agcggcaaga
     2221 gatccaacac aaccggcaag ctgatctgga aggtgaaccc cgagattgac accaccatcg
     2281 gcgagtgggc cttctgggaa accaagaaga acctcacaag aaaaatcaga tccgaggagc
     2341 tgtccttcac agtcgtcagc aacggcgcta agaatatcag cggccaatcc cccgccagaa
     2401 caagcagcga ccccggcaca aatacaacca ccgaggacca caagatcatg gcttccgaaa
     2461 acagctccgc catggtgcaa gtgcatagcc aaggcagaga ggctgctgtg agccacctca
     2521 caacactggc taccatcagc accagccccc agtctctgac cacaaagccc ggccccgaca
     2581 atagcaccca caataccccc gtgtacaagc tggatatctc cgaggctacc caagtggaac
     2641 aacaccatag aagaaccgac aacgattcca ccgccagcga tacaccttcc gccacaacag
     2701 ctgctggccc tcccaaggct gagaacacca acacctccaa gagcaccgac tttctggacc
     2761 ccgccaccac caccagccct caaaaccact ccgaaaccgc tggcaacaac aatacccacc
     2821 atcaagacac cggcgaagag tccgccagca gcggaaagct gggactgatt accaacacca
     2881 tcgccggagt ggccggactg attaccggcg gcagaagaac aagaagggaa gccattgtga
     2941 acgcccaacc caagtgcaac cctaatctcc actactggac cacacaagac gaaggcgccg
     3001 ccatcggact ggcttggatc ccttactttg gacccgctgc cgagggaatt tacatcgagg
     3061 gcctcatgca caaccaagac ggactgatct gcggactgag acagctggct aacgaaacca
     3121 cccaagctct gcagctcttt ctgagggcta caaccgagct gagaacattt tccatcctca
     3181 atagaaaggc catcgacttt ctgctgcaga gatggggcgg cacatgtcat atcctcggac
     3241 ccgattgctg catcgagcct cacgactgga caaagaacat cacagataaa attgaccaga
     3301 ttatccacga cttcgtggat aaaaccctcc ccgaccaagg cgataacgat aactggtgga
     3361 ccggatggag acaatggatt cccgccggca ttggcgtgac cggcgtcatc atcgctgtca
     3421 tcgctctgtt ctgcatctgc aaattcgtct tttgatgtct tcctggagct ccagtcattg
     3481 actataacgt ctcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3541 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3601 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3661 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3721 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3781 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3841 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3901 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ggatcggccg
     3961 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     4021 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     4081 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     4141 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     4201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     4261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     4321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     4381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     4441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     4501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     4561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     4621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     4681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     5041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     5101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     5161 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5221 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5281 ccacgctcac cggcaccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5341 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5401 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5461 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5521 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5581 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5641 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5701 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5761 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5821 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5881 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5941 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     6001 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     6061 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     6121 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     6181 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     6241 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     6301 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     6361 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     6421 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     6481 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     6541 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     6601 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     6661 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     6721 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattggagc
     6781 tccaccgcgg tg
//
