LOCUS       sequence-407427-        9231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, NUP98_KDM5A from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_NUP98_KDM5A
FEATURES             Location/Qualifiers
     source          1..9231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3992..4702,4703..4705,4706..4708))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4034..4055)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4383..4402
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4642..4663
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4705..4714
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4790..4993)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4819..4839)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4994..5297
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5554..5598)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5783..6016
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6376..6493
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6985..7110
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7157..7414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7416..7795
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7826..7930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7931..7999,8000..8791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8149..8168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8962..9231,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaac
     1801 tggtctcttt aagatcctcc attggtagtt tgtagctcat tatgaaggaa ggaggtggtg
     1861 ctggacctgg gctaactggc ccctgcttct ttgcacagtt tatacagatg taatcttcat
     1921 tttcagccat ttctggagat acacccacac aaacttgatg aaaccactca tcacagccac
     1981 catcacattg tacccagtct accttgtcct tgcagggcct ttggcagttc tgtgctgcgc
     2041 acacagcatt ctcatcatca gactcctctg ctcctgacca gtcatacttt gaggggatgt
     2101 ccagcacctt tttctctctt ttcttttcag tgctctcttt cacaagttca actttggctg
     2161 cagcagcctt ctccttcttt ttctttctct cttcttcttt tgctagtttc ttggccagtt
     2221 tattcagctc ctttgatttg tctgcaccta attttaattt cttctttcta ggtttgtcca
     2281 ttttctttaa ctccttggac ttctgttttc cttctccaaa aagttgctct accttttcta
     2341 gcttccgttt ccgtttcttc tctgaagagt cctttccttt cacttttagt ggtttctctt
     2401 ccatgctgtc atcctcttcc ttcttcttag ggtctgacat cggattccgg aagagaggag
     2461 agtctccaaa aggtgagtat gttagactat tgatgtgctg ctggagaaca gcctgctggg
     2521 cagcagaagc atttggatct gtcaaagcta ctggggcctg gggggctcca aagcccaaag
     2581 tggctgtcgt agtattaaat ccaggggccc caaaggctcc tgtaccaaga ggccctccaa
     2641 tcttaggttg gttgttccca aacaaagatg cctgtccagc accaagagct gttccaaatc
     2701 ctgcaccaag cccagttcca agagtcccag gtgctggttt acttccaaaa atactattcc
     2761 cactggtatt tgtgccaaaa ccaaagagcc cgccactggt tgtaccaaat gaaggtgcac
     2821 tggttgtgct gcttccaaat gtagtaagct tgttattgcc aaacagggtc gaaccaacag
     2881 caccaaatcc agtattggtc tgcccaaaga gacctgttcc tgttccaaat gctgtcccag
     2941 tgctggtgtt tgtagctgtc ccaaaaagac ctccaggctg tgaggcttgg gttactccaa
     3001 ataatcccat ggtgttggtg cttggctgtc ctatggtgct ggtattacca aaggaaaagc
     3061 cagtgttctg ggtggttgta gcctggccaa atggtttgct gaagaggctg gtagtctgct
     3121 gattctgttg gccaaagaga ccacctggat ttgttccaaa tccagttgta ctagttccaa
     3181 aggcagtttt gttctgacca tatgcaaagc ctgaattagt ggtggaggag ctgaagagtc
     3241 ctgttgcgct ggaagtggct ggagaagacc caaacaagcc agttgtggta cctgctccca
     3301 cctggttctg tgggcccttc ctgttagcct gataatcctc taaacgaagt tcctctagtg
     3361 acttgctttc atattctttc atagcagtaa tacactggtg cttggtactt atgttagtgc
     3421 taactccagc tttgaccata gtatctgtac cagttggagg gttaaattta atagtagtcc
     3481 cagtaggagc agctgtaaaa ctacttggcc caaagaggga gccagatgtg ctgccaaaag
     3541 gattagaggt ggtatttgtg gttccaaaga gtcctccact gctagtactg gttccaaaat
     3601 tgccaaagcc agttggttta ttttgtgcaa aggcattgtt ttgggatgag aagagactgg
     3661 tccctgtgct tgcagttcca aacaaggtat ttgctgttcc tgttgacgta ccaaacccaa
     3721 agccagtgct tgtggaggta gctggctggc taaatgaact ggttccaaac aatcctcctg
     3781 gtttagtctg tgaatttcca aagaggcctc cagtattgtt gctagaacca aatgcagatg
     3841 ttccaaatgc ccctccacta gtagtgccaa agccagtatt ctgtccaaat gttgaagttg
     3901 tgccaaagcc acctgtgcca cccccaaagg gtgttccaaa tgatttgtta aacatagcgg
     3961 ccgcacttcc cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga
     4021 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     4081 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     4141 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     4201 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     4261 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     4321 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     4381 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     4441 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     4501 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     4561 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     4621 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     4681 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta
     4741 ccgtcgactg cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta
     4801 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     4861 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     4921 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     4981 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5041 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5101 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5161 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5221 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5281 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5341 attactatta ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag
     5401 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     5461 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     5521 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     5581 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     5641 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     5701 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     5761 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     5821 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     5881 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     5941 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     6001 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     6061 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     6121 tctccaattg tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta
     6181 atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct
     6241 aatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac
     6301 tgccccttca cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc
     6361 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     6421 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     6481 ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc
     6541 ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt ctactactcc
     6601 ttgactttgg ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt
     6661 tttcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6721 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6781 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     6841 tgtttctaac aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat
     6901 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     6961 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7021 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7081 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7141 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7201 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7261 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7321 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7381 tcgccactcc ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg
     7441 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     7501 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     7561 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     7621 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     7681 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     7741 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcgaccc
     7801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     7921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     7981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     8821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     8881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     8941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg g
//
