LOCUS       sequence-407430-        9186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_BZW1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_BZW1
FEATURES             Location/Qualifiers
     source          1..9186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             3010..3021
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(join(3947..4657,4658..4660,4661..4663))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3989..4010)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4338..4357
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4597..4618
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4660..4669
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4745..4948)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4774..4794)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4949..5252
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5509..5553)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5738..5971
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6331..6448
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6940..7065
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7112..7369
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7371..7750
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7781..7885
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7886..7954,7955..8746)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8104..8123)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8917..9186,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaat
     1801 ccccttcctc agcctctgac tctgattcct cctcggcatt ctttaaccac tctacaaact
     1861 ttttcatctg ctctaagaaa acactcttgc ctttcgcaac gtgcgcgtcc ttataccact
     1921 tcagaatcgg ttcctcacta agcacctcag ccttgtagaa taaaaccaca atcttctgga
     1981 acgccttcat aaagtggata ttatcataac aatattcttg gattttcaat aacagagtaa
     2041 gttccgactg gccttgcgtt gtaaaggccg ccaataatgg ggaatactgc tttaaatgct
     2101 taatggcttg ttccgcaact aattcctcct ttttattcca ctcgaccgtg ctcattaccg
     2161 atgaccatac gatcccgatg accaccggct cagggatatt gttctttttc atctcttctt
     2221 tgacatacaa aatgatgtct ttaaagggat cgccgcgaga catttgttcc tgtaactcct
     2281 tttgaagctc tttgcgcgca ccaatggttt gttggttgcg gacgtattca ctcagctctt
     2341 ttaaaccagc ctcggtaaag tatttcgtaa agtgctcgac agactgtttg ttggcaggga
     2401 acagttccat aaggcggtta tccatagata ctttgcgaag gctggcagcc accgcattaa
     2461 tgtccttctc attgatccat gacttgaata attttaccgc gaatgctgct gacacccctt
     2521 ccttaaccag attctcgttg tacagcgagt tcaagatcga tgcattaagt gtgccgttcg
     2581 ctaacaggac ccctgttaac atggccagct tattacgctc gctttccgag aatcctttca
     2641 aaaaaagcaa caattttttc acttcatcct caaaaccctt ttccaggtac ttgtagcggc
     2701 ggataagttt gttgaagact tgggcgaatg cttgcatagt ctccaagtcc tcctgggcgg
     2761 caaatacgca cacgtcagtg cgcatcatat catcggccaa agttccccca ggtgcaagca
     2821 tacctccggc gacaaggatg tcaaacaatg tctctgcgta gcgacgatag tccagctttg
     2881 caccggacgc atctaagaat tttgccaccg cttcaaggtc cgttccggtt tctgtcagac
     2941 cctggataat acaatcctga aattgggttg ggtcgaaacg ctctttctcg tccgtgacaa
     3001 taggatagga ttgagggccg ggtacggagg acccgatgtc ggctggcgtg ggagatgcaa
     3061 gattggcctt catgtcgtcc agcccacctg caaggcttgc cattgcggaa tattccgttg
     3121 tctgctccgg tttgatgggt tcagttgtcg tgaaaatgtc gctataatgc tggcgctcga
     3181 acacgcggtc cagcacctcc aactgttgct gtgtaaacag atccccacgc atctgtttac
     3241 gcaaaaaatc gcgtccgggc aggctgtgtc cgttcgggac tggcgactct tggatacctt
     3301 cgtcacgctt acgcttgttc gtgtccgcgc ttggactggt aatacctaaa attccggaaa
     3361 tgctataaga actacctgca gagtctgtgc tcacgctgga gacctgtgtg acgctcccag
     3421 ttgacacaat agaatgagaa ctggccggca ccggttgatt agggggttgt tgaactttag
     3481 tacggatgat gcgattgatt gaagagacac taggcacagt gtcattgtcg caaacacgtt
     3541 ccgccaataa acgatcacgg atttcccaag caaacatcgt tggattctgg cgtttatatt
     3601 ccgcgatctt ctcgacaacc ttgggagtcg ctactttagg tttgctaccc ccgattacgc
     3661 cgggcttaat agagccggtc tcatagtagc ggccaaggat tttggacacg caaccgtgcg
     3721 atacacgaag ttgacgtgaa atgtcacaag gacgaacacc ttgatgcgca agttctacaa
     3781 tacgttgacg aaccacatca ggcaaagggc gtccgttaac aaatacacct cccagttgat
     3841 tgacaccgcc gtgcccggtg cgggaggtgc gcggtgtggg gtagttcttt tctaaatcca
     3901 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     3961 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     4021 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     4081 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     4141 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     4201 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     4261 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     4321 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     4381 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     4441 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     4501 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     4561 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     4621 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     4681 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     4741 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     4801 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     4861 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     4921 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     4981 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     5041 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     5101 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     5161 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     5221 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     5281 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     5341 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     5401 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     5461 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     5521 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     5581 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     5641 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     5701 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     5761 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     5821 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     5881 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     5941 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     6001 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     6061 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     6121 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     6181 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     6241 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     6301 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     6361 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     6421 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
     6481 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
     6541 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
     6601 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     6661 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     6721 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     6781 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
     6841 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     6901 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     6961 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     7021 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     7081 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     7141 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     7201 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     7261 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     7321 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
     7381 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     7441 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     7501 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     7561 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     7621 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     7681 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     7741 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7801 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7861 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7921 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7981 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     8041 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     8101 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     8161 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     8221 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     8281 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     8341 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     8401 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     8461 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     8521 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     8581 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8641 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8701 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8761 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8821 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8881 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8941 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     9001 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     9061 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     9121 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     9181 taccgg
//
