LOCUS       sequence-407432-       11706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_ZNF521 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11706)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_ZNF521
FEATURES             Location/Qualifiers
     source          1..11706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(6467..7177,7178..7180,7181..7183))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6509..6530)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6858..6877
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7117..7138
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7180..7189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(7265..7468)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7294..7314)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7469..7772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(8029..8073)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8258..8491
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8851..8968
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9460..9585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9632..9889
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        9891..10270
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10301..10405
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10406..10474,10475..11266)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10624..10643)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11437..11706,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggtctaac
     1801 tgctgtgttg ggtcattgta tgattctgca gctctgtttg gaagaaaaac ttctgtggac
     1861 attgtgtaca gtcatagatc ttgtcttctt gtccatgggc agagaaaata tgctgctgca
     1921 acttgtttgc ttgaacaaat actgtaaagc agactggaca cttgaaggtg cctcccatcc
     1981 cttcgaagct gtgctctatc aggtggcact ggagtttggc aggagagtca aaggtctggc
     2041 tgcagagttt gcattcatgg ttcagtcctt catcaatcat gtgatttgca acatgaacct
     2101 gaatatccca ttcattgtag aaaaccatct gacacttgat gcattgatag gtcttcttct
     2161 catccgactg ggagggactg attctgggca ttggtgatac ttggggcgtt ttcaactgtg
     2221 tgctgttgct gtctggcacg agctctcggt ggatggtttg gatgtggttc tggagttcac
     2281 tttcagactc aaacttaacg ttgcagctag agcagcgtgt cttcagtccc cccaccttgc
     2341 ctttcccctc aatggcactc agattctcat tctggcccaa gcctggtcta ttcgtgccgg
     2401 gagggacgtt aatgcctggg ctggcgctct tactgagatt cacgcagccg gcacacagac
     2461 catatggcag gccattgata tcaagtttca ccagatcttg cttggaacgg aattctttga
     2521 ggcaagatgc gcacttatac agtttttgga cgtgctggcc ccgccctgtg gtctgaactg
     2581 cagacccatt ccctgtcttt tgcatgtgga acgtcccatg gattttgagt tccaaggtgg
     2641 aggtcactgt ctgcatgcac accacgcagc gaaagcctgt cagggaattc ctcaagtcag
     2701 ggtgcatttg gcaatgctct aaaaactcct cttcactctg gagaggcatc ttgcaaatcc
     2761 ggcagtttcc agtatcaaga ctcttactat gcgtgacttt gtgttcagta agagttaaaa
     2821 gggagggaaa ccgctctccg caaatagggc acatgtagtg tttgacaggg cctaggtggg
     2881 tctgcatatg ttcccggagg ccattttcgg agaagaaggt tcgagagcac acgttgcact
     2941 tgtaattccc tttaatgagc tcagctttct ttttcacgat ggcactttct ccaggtctga
     3001 tgttgtggtc tcggagctgg tgattctgca gcaaagtttc catagtatag gctgccccac
     3061 aaatgtcgca gccgtacata ggctcagagg tgtcaacgtc ttcttcgctc ccatcgtgac
     3121 tgttgtggga ctcctggctg ttggtcagca aagtctgcag ctccacttcc tctttctgca
     3181 cttgctcgga agctccattt gttccacagt tgggtgtctt ggtttcgaat acacagtgtt
     3241 tttctcgcaa gtgtttttct aacaaaatga tcgcatggaa ggctttgcta cagaacttgc
     3301 agttgtactt cttactgtga gtggtgatgt ggcattgcag ctccacctcg gtgccaaagg
     3361 actcaccgca gaaaatgcac ttatgcactt tcccttggtt ttccaggtgg ttgtgtttca
     3421 catggagctg caagtcagtt tcgttgcgga agtcccagtt gcaagatgtg cacctataga
     3481 ctttcttttc gttactgtgc ttcacagcca agtggagctg aatggagact tttgagtcaa
     3541 aaacttcctg gcagagggtg cagcgaaaga agacaaaggt gtgcatgtcc agcaggtgtt
     3601 tctgaaggtc atccactgat gtgaattgct tgtcacaact ctcacagatg taatacgttg
     3661 aagtgatcat aaagtgaatg gtaacatgct tcagcaagga ttcttggttg gggaattcct
     3721 tgttgcactg aggacaggtc aattttggaa gcacagtgtc gagatgagtt tttaggtgag
     3781 tctgaaagct gtctagggat gtgtacttag caccacattg attacagata tattctccag
     3841 tgggacgtgc aggtgcacct cctactgcct gcatcatctt aagagatgtc tgctctatgg
     3901 ccacaggaga tagggggctt aaggccctgg atttcttccc attgtggata taattcaggg
     3961 ccaagggaat gtttttatga ttctctttga tatgcttgtt cagtttaaga acgctgttga
     4021 atattggcga atttgtacaa taggaacaag aatagacttc tactactggt tctttgggag
     4081 tcccaagcac tggagaccca aatcgggagc cactgaggtc acaatgaacc tgtctaatat
     4141 gctcttccag ggaagagtca gtgagaaacc ccatatagca atggggacaa aagaatgcat
     4201 tactatcttt agctgcaggg tttgcaaatc catgagaaca tcggatgtgt tcctgaagag
     4261 tgttgaggtc gttgacaact tcggaacaga agttacactg gtagacaatg gcaggcatgg
     4321 cagaaacaat cagacctggg tcctgagctt catgcacttg cttaagatgt tcatttaggt
     4381 tatagagtga gggcaggacc tccaagcaat actgacaaat atgggcctgt tctggcttat
     4441 ctaagtgcat agttttcagg tgaatctgca gaactgcaag acttgaaaat aattgtttgt
     4501 tgcagtaaat acagctgtag gtaacttttg cttgtttact cgagggacca gtcatgtcag
     4561 gggtttgttg agcggccctc ttcctccctc gactctttgg gattggcggg gcagcttcca
     4621 ccatggttga gctgtccact gagaggttgg aatctggagt cgtactggac acggaggtat
     4681 agcccaccgt gaccagggaa gggctgttgc tgtgattgca tgactccggt tgctggtgac
     4741 tgtccatgtg gctgtacagt tcctcaactg tgtggaaact ctcagaacaa atgctgcatg
     4801 agttcttctt ctccccgcta tgcacctgct tcatgtggtt catgagggag gtctcctcta
     4861 cgaagagctc gtggcagtag acacactgga gggccgctcg gtcctcattt ggggagcatt
     4921 cggggtggca ctctgcaatg tgtttttgga ggtcttccgg gaagtcaaag ccttcctcac
     4981 actgactgca cttctgagtg tccttcatct tccagtcctc catcctggaa ccggactgag
     5041 agccgtcctt gttcctctca tgaacctgca tgtgtccgtg taaggaacta gaggacagaa
     5101 acccacggcg acaaatggca catttatatg gcttgttgga cgtgtgagtc tttaagtgga
     5161 tcttcaagtg atcacttctg gaaaacgcag catcacattc actgcagtgg tacttcttgt
     5221 ccccggtgtg gagttttatg tggcgatctc ggctgcgctt gtgtttgaac agcctactgc
     5281 agtaggtgca tttgaaaggc agtttgtcac tgtgactctg ctcatggtgc tttaggtagc
     5341 tgaggcggct aaacgacttg tcacagaatt gacacgggta tggaagccca gggccacctt
     5401 cttcctctcc aaaatcgcaa ccttctccat ggctagggga agtctgatcc ttgctagaag
     5461 gtgaggaagc tggccaagag caagtcggat catcttcaac atccactcca tctgtcacaa
     5521 tggggtagga ctgcgggcct ggcacactgc tcccgatgtc agcaggggtg gggctggcca
     5581 gattggcctt catgtcgtcc agcccaccag ccagcgaggc catggctgaa tactctgtgg
     5641 tctgctcggg cttgatgggc tctgtggtgg tgaagatgtc tgagtagtgc tgcctctcaa
     5701 acacgcggtc cagcacctcc agctgctgct gtgtgaacaa gtctccccgc atctgcttcc
     5761 ggaggaagtc tctgcccgga agcgagtggc cgttcggcac cggagactcc tgaatacctt
     5821 cgtctctctt gcgcttgttg gtgtcggcgc tgggggacgt gatgcccagg atgccgctga
     5881 tggagtacga cgagccggcc gaatccgtgc tcaccgagga cacctgcgtc acggagccag
     5941 tggacactat gctgtgactg gaagctggga ctggttggtt gggtggctgc tgtacttttg
     6001 tccggatgat cctgttgatg gaactgacgc taggcacggt gtcattgtca cacacccgct
     6061 ctgccagcag ccggtccctg atctcccagg caaacatggt gggattttgg cgtttatatt
     6121 cagcgatttt ttccaccact ttgggtgtgg cgacctttgg tttggatcct ccaattaccc
     6181 caggcttgat gcttcctgtc tcataatacc tgccaagaat tttgctgaca caaccatggc
     6241 tgacccgaag ctgcctggag atgtcgcagg gcctgacacc ttgatgagca agttccacta
     6301 tcctctggcg gactacatcc gggagtggcc gtccattcac aaaaaccccc ccaagctgat
     6361 tcactcctcc atgtcctgtc ctgctggtcc gaggagtcgg ataatttttc tctaaatcca
     6421 ttccccggga agcggccgca cttcccccgg agcctccgct tccgcccttg tacagctcgt
     6481 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
     6541 tctcgttggg gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca
     6601 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc
     6661 cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg
     6721 ccatgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
     6781 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga
     6841 acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct
     6901 ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt
     6961 agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg
     7021 gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct
     7081 cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga
     7141 tgggcaccac cccggtgaac agctcctcgc ccttgctcac catggtggcg accggtggat
     7201 cccgggcccg cggtaccgtc gactgcagaa ttcggttcac taaacgagcc cggttcacta
     7261 aacgagctct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
     7321 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
     7381 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
     7441 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
     7501 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
     7561 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
     7621 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
     7681 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
     7741 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
     7801 atgaccccgt aattgattac tattaataac tagcacgcgt tcgaagcttg agctcggtcc
     7861 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
     7921 cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat
     7981 gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca
     8041 tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat
     8101 taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt
     8161 gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt
     8221 ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat cctttaggta
     8281 tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa
     8341 cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga
     8401 caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc
     8461 tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac
     8521 cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata
     8581 tttatataat tcacttctcc aattgtccct catatctcct ccttatggtg ttttactaaa
     8641 cttttccatg ttctaatcct catcctgtct acttgccaca caatcatcac ctgccatctg
     8701 ttttccataa tccctaatga tctttgcttt tcttcttggc actactttta tgtcactatt
     8761 atcttgtatt actactgccc cttcaccttt ccagaggagc tttgctggtc ctttccaaag
     8821 tggatttctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     8881 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     8941 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtactgctgt
     9001 cttaagatgt tcagcctgat ctcttacctg tcctataatt ttctttaatt ctttattcat
     9061 agattctact actccttgac tttggggatt gtagggaatt tccaggtctg aagatcctgc
     9121 tgcttgctgt gcttttttct tacttttgtt ttgctcttcc tctatcttgt ctaaagcttc
     9181 cttggtgtct tttatctcta tcctttgatg cacacaatag agggttgcta ctgtattata
     9241 taatgatcta agttcttctg atcctgtctg aagggatggt tgtagctgtc ccagtatttg
     9301 tctacagcct tctgatgttt ctaacaggcc agtattaact gcgaatcgtt ctagctccct
     9361 gcttgcccat actatatgtt ttaatttata ttttttcttt ccccctggcc ttaaccgaat
     9421 tttttcccat cgcgatctaa ttctcccccg cttaatactg acgctctcgc acccatctct
     9481 ctccttctag cctccgctag tcaaaatttt tggcgtactc accagtcgcc gcccctcgcc
     9541 tcttgccgtg cgcgcttcag caagccgagt cctgcgtcga gagagctcct ctggtttccc
     9601 tttcgctttc aagtccctgt tcgggcgcca ctgctagaga ttttccacac tgactaaaag
     9661 ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac tacttgaagc
     9721 actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc agagagctcc
     9781 caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag ctgcttatat
     9841 gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc catggtaata
     9901 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     9961 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
    10021 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
    10081 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
    10141 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
    10201 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
    10261 aatcaatgtc gacccaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
    10321 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
    10381 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
    10441 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
    10501 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
    10561 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
    10621 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
    10681 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
    10741 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
    10801 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
    10861 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
    10921 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
    10981 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
    11041 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
    11101 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
    11161 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
    11221 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
    11281 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
    11341 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
    11401 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
    11461 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
    11521 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
    11581 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
    11641 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
    11701 taccgg
//
