LOCUS       sequence-407434-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PDGFB_DGCR2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PDGFB_DGCR2
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     17..36
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(255..359)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        390..769
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             771..1028
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1075..1200
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1692..1809
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2169..2402
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2587..2631
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2888..3191
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3192..3395
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3346..3366
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3471..3480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3477..3479,3480..3482,3483..4193)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3522..3543)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3783..3802)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4130..4151
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6659..6678)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6724..6779
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6778..6797)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7140..7156)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7140..7156)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7153..7175)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7164..7180
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7188..7194,7195..7212,7213..7218))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7233..7254
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7313..7508
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7359..7494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7514..7648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7551..7570)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7605..7624
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7716..7733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7887..8475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7967..7986)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8646..9252,1..185,186..254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
       61 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      121 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      181 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      241 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      301 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      361 acatttcccc gaaaagtgcc acctgggtcg acattgatta ttgactagtt attaatagta
      421 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      481 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      541 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      601 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      661 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      721 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg gaggcgtggc
      781 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
      841 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      901 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      961 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     1021 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     1081 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1141 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1201 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1261 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1321 gttaatactg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1381 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1441 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1501 gaagagcaaa acaaaagtaa gaaaaaagca cagcaagcag caggatcttc agacctggaa
     1561 attccctaca atccccaaag tcaaggagta gtagaatcta tgaataaaga attaaagaaa
     1621 attataggac aggtaagaga tcaggctgaa catcttaaga cagcagtaca aatggcagta
     1681 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     1741 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     1801 caaaattttc gggtttatta cagggacagc agaaatccac tttggaaagg accagcaaag
     1861 ctcctctgga aaggtgaagg ggcagtagta atacaagata atagtgacat aaaagtagtg
     1921 ccaagaagaa aagcaaagat cattagggat tatggaaaac agatggcagg tgatgattgt
     1981 gtggcaagta gacaggatga ggattagaac atggaaaagt ttagtaaaac accataagga
     2041 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     2101 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     2161 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     2221 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     2281 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     2341 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     2401 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     2461 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     2521 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     2581 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     2641 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     2701 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2761 tcaccattat cgtttcagac ccacctccca accccgaggg gaccgagctc aagcttcgaa
     2821 cgcgtgctag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2881 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2941 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     3001 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     3061 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     3121 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     3181 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     3241 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     3301 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     3361 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgg gctcgtttag
     3421 tgaaccgaat tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg
     3481 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3541 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3601 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3661 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3721 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     3781 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3841 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3901 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3961 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4021 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4081 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4141 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggaa
     4201 gcggaggctc cgggggaagt gcggccgctt cccggatgaa ccgttgctgg gctttgttct
     4261 tgagtctgtg ttgctacctt cgtttagtca gtgcagaggg ggacccaatt ccggaagaac
     4321 tgtatgagat gctttctgac cattccatcc gtagtttcga cgaccttcag cgtttactgc
     4381 atggcgatcc aggtgaggaa gacggcgccg aattggactt aaatatgact cgttcccatt
     4441 ccgggggcga gcttgagagt cttgcgcgcg gtcgtcgttc tttaggctcc ttaacaatcg
     4501 ccgaacccgc aatgattgca gagtgcaaaa cccgtactga agtttttgaa atttcccgcc
     4561 gcttaatcga tcgtacgaat gcgaactttt tggtttggcc gccctgtgta gaagttcaac
     4621 gctgcagtgg gtgttgtaac aatcgtaacg tacaatgccg ccctacacag gtacaactgc
     4681 gcccagtcca agtccgtaag atcgaaattg ttcgtaagaa accgattttc aagaaagcga
     4741 cggttactct ggaagaccac ttagcatgta aatgcgagac cgtggcagcc gctcgccccg
     4801 tgacgcgcag cccaggtggg agccaggaac agcgtgagtt gcgttgcaat ccaggtcaat
     4861 ttgcctgtcg cagcggtacc atccagtgta tcccgttacc ttggcaatgt gacggttggg
     4921 cgacatgcga agacgagtca gatgaagcga attgtcctga agtaactggt gaagtacgcc
     4981 cgcaccacgg aaaagaagcc gttgatccgc gccagggccg cgctcgcggt ggagaccctt
     5041 ctcacttcca cgctgtaaat gtagcacaac ctgtacgttt ctcctcgttc ttaggcaagt
     5101 gcccgactgg gtggcatcac tatgagggaa cggcttcatg ctaccgcgta tatctttcag
     5161 gtgaaaatta ctgggatgca gcccagactt gccaacgcct taacggatcg ctggcgacgt
     5221 ttagtacgga ccaggaatta cgctttgtat tagctcagga gtgggatcaa cccgaacgtt
     5281 cttttgggtg gaaagaccaa cgtaaactgt gggtagggta tcagtacgta atcactggtc
     5341 gcaaccgcag tctggaaggt cgttgggaag tggccttcaa gggatcttct gaagtcttcc
     5401 ttcccccaga tccaatcttc gctagtgcaa tgagtgaaaa tgataacgtt ttttgcgctc
     5461 agctgcagtg cttccacttc cctacattac gccaccatga cttacattca tggcacgccg
     5521 agtcatgtta cgagaaaagc agctttttat gcaaacgctc tcaaacatgt gtcgatatca
     5581 aggataacgt ggtcgatgaa ggtttctatt tcacgccaaa gggcgacgat ccgtgcttgt
     5641 cttgtacgtg ccatggagga gagcctgaaa tgtgtgtagc tgcactgtgc gagcgccctc
     5701 agggatgcca acagtatcgt aaggacccca aggagtgctg taaatttatg tgtctggacc
     5761 ccgacgggaa cagtttattt gactccatgg catcgggaat gcgtcttgtt gtctcctgta
     5821 tctcctcctt cttgatctta tcgttattgc tgtttatggt ccaccgcctt cgccagcgcc
     5881 gtcgcgagcg tattgagtcc ctgattggtg ctaacctgca tcacttcaat ctgggacgtc
     5941 gtattcctgg tttcgattac ggccctgatg gcttcggtac tggtcttacc cctttgcacc
     6001 tgtcggatga tggagagggc ggaactttcc atttccacga ccctcctccc ccttacactg
     6061 cgtacaagta cccggacatc ggtcaaccgg acgacccacc cccaccttat gaagcatcca
     6121 ttcaccctga cagcgtcttt tacgatcccg cggacgacga cgcttttgaa ccagtagagg
     6181 tatccttgcc tgctccaggg gacgggggat cagagggggc tttactgcgc cgtttagagc
     6241 agccgcttcc gaccgcgggg gcatccttag cggatctgga ggattcagcg gacagctcaa
     6301 gtgcacttct tgttcctccc gacccagcac aaagtggctc gacgccagct gcagaagcac
     6361 ttcctggggg aggtcgtcac agccgttcct ccttaaatac cgtggtatga accggttcta
     6421 gataagatta cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6481 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaaagaag acaagatctg
     6541 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6601 taactaggga acccactgct taagcctcaa taaagcttca gctgctcgag ctagcagatc
     6661 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     6721 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     6781 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     6841 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     6901 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     6961 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     7021 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     7081 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     7141 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7201 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7261 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     7321 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7381 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7441 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7501 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7561 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7621 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     7681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7981 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8701 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9241 agttgctctt gc
//
