LOCUS       sequence-407437-        9750 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, MYDGF_DNM2 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9750)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_MYDGF_DNM2
FEATURES             Location/Qualifiers
     source          1..9750
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4511..5221,5222..5224,5225..5227))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4553..4574)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4902..4921
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5161..5182
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5224..5233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5309..5512)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5338..5358)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5513..5816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6073..6117)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6302..6535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6895..7012
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7504..7629
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7676..7933
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7935..8314
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8345..8449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8450..8518,8519..9310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8668..8687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9481..9750,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagt
     1801 caagaagtga gggctctgct ggacggataa tggttgggcg ggatggagct gccggcggac
     1861 gacggctagg aacgccgggt ggaataccgg gtggaatacg gacggggcga cttggaattt
     1921 gtggcggtgc agggaaaagg tccgaattgg caaagacact ctgtggtccc ggacgtgagg
     1981 gaataggggg tgcactaaaa ctagccgctg cccctacggg gaccgggata agaggaggac
     2041 cgggagttgg accgcgcacg gccggtggac gtccaggtgg gtgaatagaa gagacggggt
     2101 ggcgctgggg agtaggagag tgagaagagg cactttgtaa ccaagtatca tcgaccggag
     2161 gaggcacagg tgtgctcaca gtactcgtag agatatcgcc gataatatta agagcctcct
     2221 ttaatgcatg atacatacgc aacatatcgt cgcgacgctg cgcctggtcc gcagattctt
     2281 ccattaatga ggattggtcg gcgctcgaat aaaggtaggc cagtaactcg tggtggataa
     2341 aggctttggt attattgatc atcaaatgca tgatagtttt aggcataaga tcgcggatag
     2401 atttattaat aatggccaca taggagtcaa ccaaattacg gatagtttcc acctggcgtt
     2461 ccagttgcgg gtccatgctg aatgtgtttt cttgtgcgcc atcttcattt tccgcctggt
     2521 ccttttcagg atacacgcca gcacgcagga aggaagcctt ccaggaatct acatcctctt
     2581 gggaatcgca cgctaactcg atctgacgca aatctttata cacgttgcgt tgctctgtgt
     2641 tgaagatcgc aaacacatgt ttgttagaca tgaaaccttt ctccacatcg cggatcttaa
     2701 ggttgtccaa aggtaacata tattttttct ccttctcctc ctcatctttg taccagctta
     2761 aagattcagc ggtcagaaca aaccaatatt cctttgaacc tcctttcatc aaagagatgt
     2821 tattgatcgt caaccatcca cggcgaatca ccaaaatttc accttgattt ggaatcgcgc
     2881 gtttcttgtt taactgagtc gagcgctgtt gcgcgtttgc aaacccaatg aagtcttcat
     2941 ggttggtgtt aatgtagctt tgctcaatgt cgataagcag taagatctga tctttggtgt
     3001 gcccttcacg ctcacgaata tacgtggtta caatgcgttc ggtctcctcg cgtaaacgag
     3061 ggtagctaga cagcttagaa gtacattgac gcacggtatt gatcagttct tggatgacca
     3121 aatctacaca cttcaagcat ggctctttta atttaacaac ctgcttctta acaatggctt
     3181 caaatgccag gtctggtgtg aacaacccgg tacgaactcc atgaatattc ttgatggcgt
     3241 aggagatctc acgacgcaag tccttttcgt caaactccat ttttacaagt tcgaagggaa
     3301 agcgttcgtg aaaaatgcgg ttaatacgcg ccccaccgct taattccagc gtgtcaacct
     3361 ggtcgcccga accttcaata cgcttctcaa agtctacacc aaactgctgt accatttgca
     3421 acagagcttt cgttttgcgt gttgggtcgt ccgggcggaa gttcttgtac tcttccacct
     3481 ccttttctaa ggacaacagc tgggattgta acttactacg cagggcaggc aaggactcgc
     3541 gaatatgatt tgttaactgc tgatttaagg ttttttgaag gtggggagtg cccatgcgat
     3601 cggccatgtg acggtaggcg gggtgggaaa ggaaaaattt acgctctgct gctaaagcag
     3661 cgcggatgtc ctttttacct tcgatgtctt tctgcgaacg attcacgacc ccaatatagc
     3721 cgcggcgcaa aggaagtaat ttattttcca atacgtcgcg ggcatccgtt ccctcatcca
     3781 tcaaatccag tttcgtaatg acgccgatgg tgcgtaagcc ttgcgggtcc acctccttcg
     3841 ccaacttaag agcgtcagaa tttgccaggt ccatattagc tggtgtgacc gcaagaatta
     3901 aagaactttc gcgtgagata aattgaagaa tcatgtcctt aatttgatac tcaatatcag
     3961 gtggctggtc gccaactggc actttagtaa tgccaggcag atcaatcagg gtcaagttca
     4021 gcacggctgt tttggtcacc tcaaactctt cagttttcag aggtacatcg gattcgcgct
     4081 cgaaggccgc cttactatat gccatcgcgt actcaatttc ggctccgcga acctctgctt
     4141 taaattgtgt gaagtacaag taagacttac cttgggggcg ccaaattgta caggtgaagt
     4201 gctggtggtc ctcgctcgtc cccaagctca tttgccactg ttcgtttgta cccccttgtg
     4261 aagcataggt aaacatgcat gtatatttgt cgccgggacc cacgttatga gaaaaactat
     4321 gtacgacccc ccctgggcgt acgtcgaagg cgactgtggt aggctcggaa actgcctctg
     4381 cgggacgtaa agcgaccgcc cctaacagca gggcagccca aagactagcc ccaactccgt
     4441 tccatccgcc cgatggtgca gccattcccc gggaagcggc cgcacttccc ccggagcctc
     4501 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     4561 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     4621 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     4681 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4741 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4801 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4861 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4921 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4981 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     5041 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     5101 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     5161 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     5221 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     5281 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     5341 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     5401 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     5461 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     5521 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     5581 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     5641 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     5701 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     5761 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     5821 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
     5881 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     5941 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     6001 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     6061 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     6121 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     6181 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     6241 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     6301 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     6361 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     6421 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     6481 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     6541 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     6601 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     6661 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     6721 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
     6781 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
     6841 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
     6901 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     6961 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     7021 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
     7081 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
     7141 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
     7201 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     7261 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     7321 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
     7381 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     7441 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     7501 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     7561 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     7621 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     7681 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     7741 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7801 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     7861 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     7921 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     7981 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     8041 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     8101 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     8161 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     8221 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     8281 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     8341 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8401 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8461 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8521 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8581 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8641 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8701 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8761 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8821 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8881 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8941 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     9001 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     9061 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9121 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9181 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9241 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9301 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9361 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9421 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9481 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9541 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9601 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9661 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9721 tgccagtggc gataagtcgt gtcttaccgg
//
