LOCUS       sequence-407440-       10965 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_AUTS2 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_AUTS2
FEATURES             Location/Qualifiers
     source          1..10965
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(2171..2194)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(3974..3991)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             complement(3980..3997)
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             complement(join(5726..6436,6437..6439,6440..6442))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5768..5789)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6117..6136
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6376..6397
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6439..6448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6524..6727)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6553..6573)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6728..7031
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(7288..7332)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7517..7750
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8110..8227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8719..8844
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8891..9148
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        9150..9529
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9560..9664
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9665..9733,9734..10525)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9883..9902)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10696..10965,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tagcgcgctt
     1801 caatgtcctt cagggtgtgc gacggcggac gctcacgaat ctctgcagat agcggggttg
     1861 tacgacgcgg ggagaccgga cggcccccga gggtgctaat cagcggcggc ggggcagaca
     1921 gcgctgccgt cggcggggtt ttgttcagca ggccgttctg gttaccggca gtggggctga
     1981 tacgcggata atgcatgctt ggcagacccg gagtgatcag ggacgggtgc ggaaggtgcc
     2041 cgtccaggct cgccggatga acggagtgca ggcgggtatg ctcgtaatcc tcgcgcagca
     2101 tgtgcaatct ttctctctcg tccaggtggc cgccgcgttc gtgctcacga cgcgggtcca
     2161 cagacaatgg gtggtggtgg tgatggtgat ggtgggagta atcatgcggc tcacgatcac
     2221 ggaagctacg atcggcttca tataacctcg gcgtactcag gcgatgcaga ggatcattac
     2281 gcaacagaaa gtcgcgaccc agcgggtcac gacgatgaat gtccagctcg cggtacggat
     2341 cacgcaacgg gtcacgaatc gggtcccaat gaaatgacgg gtacggaaaa cgctcaccac
     2401 ctggcaacgg gctaataccc atgaacgggg tcatcatgcg agtacgatcc aagctgctta
     2461 tgctattcat cggatggatg ccggtaacac ccacggtcat cggcatgctc gccagtggac
     2521 ccggatgcac agagctagag ctgtttggcg gaggcggttc cgacgctcga tgggtctgcg
     2581 gggcctccgg cggcaggtcg tggtcctctt ttctttcttc tttgacttta acctcgctgc
     2641 ttttcttcgg gttctcgtac gccggctcac ccttcctagg ctccgcctca cgggacgaag
     2701 tgctgttggg cctcgcgctc tcaacaaccg gggtccgcac gtacgggctc ggcacacgcg
     2761 ccaactgttt agcctcctca cccgccgcac gaccttcgtg gccgtggccg tccttctccg
     2821 gtaggtggcc ctccttcgcc ttatgttcat cagccgccag gtccttacgg ctttcggaat
     2881 ggtcacgttc acgttccttc ggcttgtcct tttcgcgagc ctcggtattc aggtgcgcgc
     2941 gaatctgctc ggtagagctg cgggtgtggc ccagcgcgtt caccggcagg accggagccg
     3001 gactagggtg cgacgaatgg cgcttttcga cgctttcgcg ttctttatcg tccttgctaa
     3061 cggagctgtc acgtttatcg acgtcacgat cacggtcgtg cgcagccgca ctcgcgctac
     3121 gttccaactc gcccggcttc agccacggcg gtggggtcgg gaaggatggc ggggtgcggt
     3181 ggagtctatt ccacggttca tgcgggttgc taaagttctg taccgacgga ccgtccttgt
     3241 gaccgaacat gctatttgga gttacggacg ggttgcctaa tccaccaaat gcattaccac
     3301 ccaccgccgc caagcctgta aacgtagacg gtctgttaaa cggttccaga tgggccgctg
     3361 ggttcagaaa attagagtgg tgtgggggtg gaccgaacgg ggtgcccgtc ggatgcgccg
     3421 cgcccgccgc gctgaacaac gtgcttggcc gggccaggtc atgtgggtgg tgaattgcgc
     3481 cgaacagtga cgggcccgga ggacggctca gaaactcagg tttcagaccg aagtccagtt
     3541 tatgcggatc gctctgcatc tgttttttga ctttctgttg gtgatgataa atctgccatg
     3601 caatgtgcac gtgcatcgca caccatttac ccggcttacg cagcatcgga cgaaacggat
     3661 cggtcaagcg cggatctttc tgcagcaggg tgtgcggcac cgtacccggg cgtgcagcca
     3721 catcgatcgg gttggatgtc ttcggctgaa acgcaccctg aagactaccg aacgggccag
     3781 tcggcggtat cattggcggg atgccggaaa ccgctggcgg gtatgagtgg aataagctat
     3841 gacgatagaa cgggtccacc ttggtcgggt atttgtcaaa cataggaggc gcgggggtcg
     3901 gcatgatcgc cgtcgggggg atagcgtgag ggaacggggt aaaggtgtgc tgatgggtat
     3961 gttgatgcgt gtgctggtgt tgatgttgat gttggtggaa ctccgttctt aggtacggcg
     4021 gcggaccgag gctagcaccg cgatccgcgc tctggctcgc cagaaaacgg gtattcaact
     4081 cctgcctcag gatatcctgc tctgaatagg tgctgccggc cgggtggcct gcaacttgca
     4141 gactaccgct ggtcagtggc ggagggggcg gcagcgcggt cggcggtgca aacatgttcg
     4201 gatgatggct gtgcgccggc ggttgcaagg ttggcgtaaa gctgtgcaga ggagaatggt
     4261 tcggcaggct aagcgggaac ggagacgcag acggatggtg gctgatgtgc ggcggggccg
     4321 gctgggtttt agcgggcgtc gaggaacgag aagaggacag actattcaag ctcaagctgc
     4381 tgctattgta ggcggacagc ggctgggaca ggctctgtgc agaagggtgg gcctgcaccg
     4441 gaggcaggtt ttggtgcagg agttgggttg gggactgcgg acgcggcgga cgttgaacct
     4501 gaggctgcgg cgctggttgc aactgcgctt ccggcggacc ttgaggcggc tgagtgctca
     4561 atggcggcgg ttgcggaggt gcttccgtac gctgaaccaa atccgggtcc ggagacggag
     4621 cacgcagctg cggttcggtt tgcggttgcg ggatcggctg cgcaacctgc gggcacgggt
     4681 ctttcagaac cacaggttca aagatgggct ctttgcagca atcttggctt ttttcttggc
     4741 tacgttccag accggagatt ttgggcacaa tcgggccggc gtcttgtgaa tcgtgctccg
     4801 gcagagttcc tacgcctaac tccgggtcct tgttgacgat cacggtgttg aacagctttt
     4861 cggaagaagc gtccgatgcc ttttcctcct ggtctgaatc gctatcacat tgctccggtt
     4921 tgatcggctc ggtcgtggta aagatgtccg aataatgttg acgctcgaac acgcgatcca
     4981 gaacttccag ttgctgttgc gtgaaaagat caccacgcat ctgcttgcgc aggaaatcac
     5041 ggcccggtaa gctatggccg ttcgggaccg ggctctcctg gataccttcg tcgcgcttcc
     5101 gcttattggt atcagcagac ggagaggtaa tcccgaggat accgctgatg ctgtaggagc
     5161 tacccgcaga atcggtggaa acgctgctaa cctgggtaac ggagccggtg ctcacgatgg
     5221 agtggctgga agccggaacc ggttggttcg gaggttgttg gaccttggta cgaataatac
     5281 ggttgatgga tgaaaccgac ggaacggtgt cgttgtcgca cacgcgttcg gccaacagtc
     5341 tgtcacgaat ttcccaggcg aacatcgtag ggttctgacg tttatactcc gcgatctttt
     5401 ccaccacctt gggggtagca actttcggtt tcgagccgcc gatgacaccc ggtttaatgg
     5461 aaccggtttc gtaataacga cccagaattt ttgaaacaca gccgtgcgaa acgcgaagtt
     5521 gacggctgat gtcgcaaggt ctcacgcctt gatgtgccaa ctccacaatg cgctgacgta
     5581 caacatctgg cagcggacgg ccattcacga aaacgccgcc caactgattc acaccgccgt
     5641 gaccggtacg gctggtgcgg ggtgttggat aatttttttc taaatccata gcggccgcac
     5701 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     5761 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     5821 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     5881 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     5941 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     6001 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     6061 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     6121 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     6181 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     6241 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     6301 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     6361 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     6421 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     6481 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     6541 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     6601 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     6661 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     6721 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     6781 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     6841 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     6901 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     6961 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     7021 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     7081 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     7141 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     7201 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     7261 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     7321 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     7381 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     7441 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     7501 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     7561 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     7621 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     7681 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     7741 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     7801 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     7861 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
     7921 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
     7981 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
     8041 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
     8101 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     8161 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     8221 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
     8281 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
     8341 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
     8401 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     8461 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     8521 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     8581 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     8641 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     8701 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     8761 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     8821 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     8881 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     8941 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     9001 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     9061 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     9121 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
     9181 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     9241 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     9301 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     9361 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     9421 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     9481 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     9541 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9601 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9661 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9721 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9781 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9841 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9901 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9961 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    10021 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    10081 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    10141 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    10201 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    10261 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    10321 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    10381 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    10441 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    10501 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10561 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10621 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10681 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    10741 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10801 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10861 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10921 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
