LOCUS       sequence-407441-        9057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_JAK2 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9057)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_JAK2
FEATURES             Location/Qualifiers
     source          1..9057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3818..4528,4529..4531,4532..4534))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3860..3881)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4209..4228
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4468..4489
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4531..4540
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4616..4819)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4645..4665)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4820..5123
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5380..5424)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5609..5842
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6202..6319
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6811..6936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6983..7240
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7242..7621
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7652..7756
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7757..7825,7826..8617)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7975..7994)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8788..9057,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaac
     1801 cagccatatt gtcgcggatt tgatctacac gaagcgcaag gtcgcggaaa ctcggacgct
     1861 gattaacatt gttgttccaa cactcggtca tgatcatgta aatttcgtca gggcacccgt
     1921 ctggacgagg taagcggcca ttgtttttta ataactcaat caaatgaaaa acaatcatct
     1981 gtccctgctt atcgttccca atcatacgca taaattcagc cgggggcgat ttcgattttt
     2041 cgatgtaggt aaacaactca tacagtacca ccccaaaact ccatacgtcg gatgcgacac
     2101 taaacttaga ttccgtcagt gactctgggg cataccagaa gatgggtgac tcccccggct
     2161 ctttgacttt gtagtattct ttatcttgag gcaaaacctt ggtaagaccg aaatccccga
     2221 tttttacacg gttctcattc tctacaagga tgttacgggt cgccaaatcg cggtgaatat
     2281 aacgcttcgt accaaggtac tccattccct tacagatctg gcttgtatat tgcaataact
     2341 tgatgtgatc aatacgttct ttgtgttttt gcaggtaatc gcgcaatgac ccgtagggaa
     2401 ggtattccat gatcaacttc aggttacgac gccccgcact atagcaaaca cctttatact
     2461 tgacgatatt atcatgttga agtgatttaa ggatctcgat ctcgcgttca aaatcacgaa
     2521 gatgctcttc ggtagaatgt tgaagtttct tcacagcaac gacctcgcca gtgttatcct
     2581 gaagcgggtc gtagcgacac atttcaacgc taccaaagtt acctttaccc aactgctgca
     2641 agaacttcaa gtgacgttct tcaaactgtg tcgggtcacg gtcttcaaaa gctccactaa
     2701 agcctaatgc gccgatacgc atattgggaa gcatgtcatt ttcggtcagc aactcgtagt
     2761 cggggaccat gcccgtcagc gtaggcgcgc tatatgagcc ctgtccagcg ggcggaacat
     2821 ggggcgggta tccgggcaat gtggttgatg ctaaatcgcg ccccgttaca ataggatacg
     2881 actgcggccc tggaacggat gacccaatgt ctgcgggtgt tggagacgct aaattggcct
     2941 tcatgtcatc cagtcctccg gccaagctag ccatcgcgct atactcagtc gtctgttcag
     3001 gtttaattgg ctcagtggtt gtaaaaatat cactgtagtg ctggcgttcg aaaacgcgat
     3061 ctaagacttc aagctgctgc tgagtaaaca ggtcgccgcg catttgctta cgcaggaagt
     3121 cgcgtcccgg cagagagtgt ccgtttggca cggggctttc ctggatgcct tcatcgcgtt
     3181 tacgcttgtt agtatcggcc gaaggtgagg taattcctaa aattccagag atgctataac
     3241 ttgacccggc gctgtcggtt gaaacactcg atacttgtgt taccgacccc gtagacacga
     3301 tcgaatggga gctcgctggc acgggttggt taggcggctg ttgcacttta gtgcgaatga
     3361 tgcgattaat cgacgaaacc gatggaaccg tgtcattatc gcaaacacgc tccgccaaca
     3421 gacggtcgcg aatttcccag gcaaacatag tcgggttttg gcgtttgtac tctgcaatct
     3481 tctctacgac cttcggtgtg gcgacctttg gcttggaacc cccgatgaca cccggcttga
     3541 tcgagccagt ctcataataa cgacccagaa tttttgatac gcagccgtgc gaaacgcgta
     3601 attgacgcga aatgtcgcaa gggcggacac cctggtgcgc taactctacg atacgctggc
     3661 gcaccacatc cggaagcggg cggccgttga cgaaaacccc cccaagctgg ttaaccccac
     3721 catgccccgt gcgtgaggta cgcggggtag gatagttctt ctctaagtcc attccccggg
     3781 aagcggccgc acttcccccg gagcctccgc ttccgccctt gtacagctcg tccatgccga
     3841 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
     3901 ggtctttgct cagcttggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
     3961 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
     4021 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
     4081 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     4141 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     4201 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     4261 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     4321 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     4381 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     4441 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     4501 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gaccggtgga tcccgggccc
     4561 gcggtaccgt cgactgcaga attcggttca ctaaacgagc ccggttcact aaacgagctc
     4621 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     4681 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     4741 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     4801 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     4861 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     4921 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     4981 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     5041 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     5101 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     5161 taattgatta ctattaataa ctagcacgcg ttcgaagctt gagctcggtc ccctcggggt
     5221 tgggaggtgg gtctgaaacg ataatggtga atatccctgc ctaactctat tcactataga
     5281 aagtacagca aaaactattc ttaaacctac caagcctcct actatcatta tgaataattt
     5341 tatataccac agccaatttg ttatgttaaa ccaattccac aaacttgccc atttatctaa
     5401 ttccaataat tcttgttcat tcttttcttg ctggttttgc gattcttcaa ttaaggagtg
     5461 tattaagctt gtgtaattgt taatttctct gtcccactcc atccaggtcg tgtgattcca
     5521 aatctgttcc agagatttat tactccaact agcattccaa ggcacagcag tggtgcaaat
     5581 gagttttcca gagcaacccc aaatccccag gagctgttga tcctttaggt atctttccac
     5641 agccaggatt cttgcctgga gctgcttgat gccccagact gtgagttgca acagatgctg
     5701 ttgcgcctca atagccctca gcaaattgtt ctgctgctgc actataccag acaataattg
     5761 tctggcctgt accgtcagcg tcattgacgc tgcgcccata gtgcttcctg ctgctcccaa
     5821 gaacccaagg aacaaagctc ctattcccac tgctcttttt tctctctgca ccactcttct
     5881 ctttgccttg gtgggtgcta ctcctaatgg ttcaattttt actactttat atttatataa
     5941 ttcacttctc caattgtccc tcatatctcc tccttatggt gttttactaa acttttccat
     6001 gttctaatcc tcatcctgtc tacttgccac acaatcatca cctgccatct gttttccata
     6061 atccctaatg atctttgctt ttcttcttgg cactactttt atgtcactat tatcttgtat
     6121 tactactgcc ccttcacctt tccagaggag ctttgctggt cctttccaaa gtggatttct
     6181 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
     6241 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     6301 cccccctttt cttttaaaat tgtggatgaa tactgccatt tgtactgctg tcttaagatg
     6361 ttcagcctga tctcttacct gtcctataat tttctttaat tctttattca tagattctac
     6421 tactccttga ctttggggat tgtagggaat ttccaggtct gaagatcctg ctgcttgctg
     6481 tgcttttttc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     6541 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     6601 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     6661 ttctgatgtt tctaacaggc cagtattaac tgcgaatcgt tctagctccc tgcttgccca
     6721 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     6781 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     6841 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     6901 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     6961 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     7021 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     7081 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     7141 atctggtcta accagagaga cccagtacag gcaaaaagca gctgcttata tgcaggatct
     7201 gagggctcgc cactccccag tcccgcccag gccacgcctc ccatggtaat agcgatgact
     7261 aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc
     7321 caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact
     7381 tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa
     7441 gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg
     7501 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat
     7561 gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt
     7621 cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7681 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7741 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7801 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7861 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7921 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7981 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     8041 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     8101 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     8161 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8221 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8281 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8341 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8401 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8461 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8521 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8581 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8641 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8701 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8761 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     8821 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     8881 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     8941 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     9001 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgg
//
