LOCUS       sequence-407445-        9628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_ESRRA from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9628)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_ESRRA
FEATURES             Location/Qualifiers
     source          1..9628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             2281..2292
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3602..3613
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(join(4389..5099,5100..5102,5103..5105))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4431..4452)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4780..4799
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5039..5060
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5102..5111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5187..5390)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5216..5236)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5391..5694
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5951..5995)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6180..6413
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6773..6890
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7382..7507
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7554..7811
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7813..8192
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8223..8327
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8328..8396,8397..9188)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8546..8565)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9359..9628,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagt
     1801 ccatcatggc ctccagcatc tcaagaaata acttgtgcat cgggaccttt ccttccaact
     1861 taaccccata gaaatgagcc aacactttgc ccgctgtttg gcgtaataac ggtaaggtta
     1921 acagcaagcg acctgcgcgg cgacgctctg cgcctcctcc cggtcccgca cgtccagctt
     1981 catactccaa taatgcttcg tgaagagctt cgcgcaattg ttctaccgcc tccgcatcct
     2041 caatgtgcac gctatcggag tttgccaggg caagggcctt aagtaacacg tattcctcgc
     2101 gttctaagcg aagagcctgt aaacgacgga ctaattgaag caaggctgcc cctaactcac
     2161 ctaacccggc ggcgcgcgcg ccttcttcgt caagaaccaa atcctctgca aacgctaatt
     2221 catcttgtaa aggcaagctg cgttgcgcga ctcccaaaac cagaacctcc atccataccg
     2281 attgaaggac gctcatctgg tctgacaatg acagactgct aaatccgggg attgatttcg
     2341 cccaactgat tgtgactaca atttcacgat caaataagtc gcacagtgtt gctacagctg
     2401 gcaggtgtcc atcaggacct gctggatcag gcatcgcata caacttctct ggttctacta
     2461 ccaacaaatg cgagactaaa gcatttaccg gggctgcagt tttacgcgga cctcccgcga
     2521 ctgccagggg gcctgctggg aatgggccgg gaaagggtaa agggtctacc tcgggacggc
     2581 gcttatactt ctgacgtccc ccacgcacac ggtccaagcg aacgccctcc ttcagcatac
     2641 ctacacgaag acattttgta aagcggcaag cctgacaggc tttacgacga cgcttcgtga
     2701 tttcacattc gttactggct ggacaagagt actcgataga accctcgtcg cgtttacgct
     2761 tattggtatc ggcgctcggc gaggtgatgc ccaaaatgcc ggagatacta tacgagctac
     2821 ctgcagagtc cgtcgaaacg ctactaactt gtgtaacaga acctgtggac acgatcgagt
     2881 gagatgaggc aggaacgggc tgattgggag gctgctgtac cttcgtgcgg atgatgcggt
     2941 tgatgcttga aactgacggt acggtgtcat tatcacaaac acgctcagcc agcaaacgat
     3001 cgcggatctc ccacgcaaac attccccggg aagcggccgc cttgtacagc tcgaagggcg
     3061 aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga accggttcag
     3121 tccatcatgg cctccagcat ctcaagaaat aacttgtgca tcgggacctt tccttccaac
     3181 ttaaccccat agaaatgagc caacactttg cccgctgttt ggcgtaataa cggtaaggtt
     3241 aacagcaagc gacctgcgcg gcgacgctct gcgcctcctc ccggtcccgc acgtccagct
     3301 tcatactcca ataatgcttc gtgaagagct tcgcgcaatt gttctaccgc ctccgcatcc
     3361 tcaatgtgca cgctatcgga gtttgccagg gcaagggcct taagtaacac gtattcctcg
     3421 cgttctaagc gaagagcctg taaacgacgg actaattgaa gcaaggctgc ccctaactca
     3481 cctaacccgg cggcgcgcgc gccttcttcg tcaagaacca aatcctctgc aaacgctaat
     3541 tcatcttgta aaggcaagct gcgttgcgcg actcccaaaa ccagaacctc catccatacc
     3601 gattgaagga cgctcatctg gtctgacaat gacagactgc taaatccggg gattgatttc
     3661 gcccaactga ttgtgactac aatttcacga tcaaataagt cacacagtgt tgctacagct
     3721 ggcaggtgtc catcaggacc tgctggatca ggcatcgcat acaacttctc tggttctact
     3781 accaacaaat gcgagactaa agcatttacc ggggctgcag ttttacgcgg acctcccgcg
     3841 actgccaggg ggcctgctgg gaatgggccg ggaaagggta aagggtctac ctcgggacgg
     3901 cgcttatact tctgacgtcc cccacgcaca cggtccaagc gaacgccctc cttcagcata
     3961 cctacacgaa gacattttgt aaagcggcaa gcctgacagg ctttacgacg acgcttcgtg
     4021 atttcacatt cgttactggc tggacaagag tactcgatag aaccctcgtc gcgtttacgc
     4081 ttattggtat cggcgctcgg cgaggtgatg cccaaaatgc cggagatact atacgagcta
     4141 cctgcagagt ccgtcgaaac gctactaact tgtgtaacag aacctgtgga cacgatcgag
     4201 tgagatgagg caggaacggg ctgattggga ggctgctgta ccttcgtgcg gatgatgcgg
     4261 ttgatgcttg aaactgacgg tacggtgtca ttatcacaaa cacgctcagc cagcaaacga
     4321 tcgcggatct cccacgcaaa cattccccgg gaagcggccg cacttccccc ggagcctccg
     4381 cttccgccct tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc
     4441 agcaggacca tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc
     4501 aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag
     4561 tggtcggcga gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg
     4621 atgccgttct tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc
     4681 agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg
     4741 ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc
     4801 ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg
     4861 tgctgcttca tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc
     4921 acgagggtgg gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc
     4981 ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg
     5041 tcgccgtcca gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc
     5101 accatggtgg cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcggttc
     5161 actaaacgag cccggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc
     5221 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
     5281 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     5341 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
     5401 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     5461 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     5521 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     5581 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     5641 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     5701 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagcacgc
     5761 gttcgaagct tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg
     5821 aatatccctg cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta
     5881 ccaagcctcc tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa
     5941 accaattcca caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt
     6001 gctggttttg cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc
     6061 tgtcccactc catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac
     6121 tagcattcca aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca
     6181 ggagctgttg atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga
     6241 tgccccagac tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt
     6301 tctgctgctg cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg
     6361 ctgcgcccat agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca
     6421 ctgctctttt ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg
     6481 gttcaatttt tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc
     6541 ctccttatgg tgttttacta aacttttcca tgttctaatc ctcatcctgt ctacttgcca
     6601 cacaatcatc acctgccatc tgttttccat aatccctaat gatctttgct tttcttcttg
     6661 gcactacttt tatgtcacta ttatcttgta ttactactgc cccttcacct ttccagagga
     6721 gctttgctgg tcctttccaa agtggatttc tgctgtccct gtaataaacc cgaaaatttt
     6781 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
     6841 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga
     6901 atactgccat ttgtactgct gtcttaagat gttcagcctg atctcttacc tgtcctataa
     6961 ttttctttaa ttctttattc atagattcta ctactccttg actttgggga ttgtagggaa
     7021 tttccaggtc tgaagatcct gctgcttgct gtgctttttt cttacttttg ttttgctctt
     7081 cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat
     7141 agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg
     7201 gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccagtattaa
     7261 ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct
     7321 ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac
     7381 tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac
     7441 tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc
     7501 gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga
     7561 gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag
     7621 acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt
     7681 ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca
     7741 ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca
     7801 ggccacgcct cccatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     7861 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     7921 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     7981 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
     8041 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
     8101 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
     8161 ttactattaa taactagtca ataatcaatg tcgacccagg tggcactttt cggggaaatg
     8221 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8281 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8341 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8401 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8461 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8521 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8581 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8641 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8701 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8761 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8821 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     8881 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     8941 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9001 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9061 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9121 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9181 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9241 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9301 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9361 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9421 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9481 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9541 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9601 ccagtggcga taagtcgtgt cttaccgg
//
