LOCUS       sequence-407446-        9801 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_CBFA2T3 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9801)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_CBFA2T3
FEATURES             Location/Qualifiers
     source          1..9801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4562..5272,5273..5275,5276..5278))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4604..4625)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4953..4972
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5212..5233
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5275..5284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5360..5563)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5389..5409)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5564..5867
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6124..6168)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6353..6586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6946..7063
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7555..7680
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7727..7984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7986..8365
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8396..8500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8501..8569,8570..9361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8719..8738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9532..9801,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 gtggtacggt gtccaaaggt cccggtggtg aaggggaacc tggacggctg ggaccagcag
     1861 accctgcttc ggagggacta gccgctccga caggcaatga gggccccaac gaatgagcgg
     1921 cttctggcgg accaggtacc gggtcagcaa caacggcggt agggccctgc aatgattgac
     1981 cacacacgtg gtggtgcttc tcccaatcgc gatgctgaca gaagctgccg caatagcgag
     2041 cagcattgca acccgagcac gtttcactgg ctttacgtcc acagttccaa cacgactcac
     2101 tggagtcttc ctgttgattg ataacagtaa gagcgtcttc actcgcttga cgtttagctt
     2161 cggcaagtgc acgttccatt ttggcgcgct ctgttgtgat taattcgtgg gccttacgtt
     2221 cggcgtcgct aaccgccttc tgcaattcac tcatggcctg gcgtttaacc tcatttactg
     2281 cctcttccgc cttgcgccaa atatcttccg gaacgtaccc ggtaagcgtg cgtggtaaaa
     2341 actcgcgtgg gacgtccagt tgaggaccct ccggcccggc agatgacgag cgcgggcgtg
     2401 cagcagctgg tgccggcccc ttttttgtat cctccgcgtc cgaatagcgg cgcgcccaat
     2461 ggtttaattc ctcacggtct gcttcctgac aacgacgaag cacagttaat gaacgacgag
     2521 ttttttcgac catatccata atgcagttca aaagattgtt aaggtgcttc cattcttcag
     2581 cccactcgcg ttctgttaac ttatgatcga ttacttcttc ctggcgggac ccaggtacca
     2641 ctaacgggcg atggcgctcg cgcaattcac gggggtccgg gtgacgatac gcgtcacgaa
     2701 agtggtgcgc catagcgatg tcctccaaac gataatgagg tggtggcgtc ggctgcgggg
     2761 gtccattgga aggggagtag cgttgagctg gatttaaagt gcaggggcgc tttgacaaat
     2821 gctcgggatg cagagggtcg cgatcagatc cgttctcctt agtacggtcc ggagtacggc
     2881 gtttaccatt ctcatttact tccaaaagta attccgagga atcaatagga ctggacgcac
     2941 tggcatccag taacaactgt tcgtgctgtg ccaagtattg ggccggggtc tgcttagcca
     3001 agcgtgcgca gtgaagaagt tcgcgctgaa gtaatggtaa gttagctttt aagaagggga
     3061 taacaaaagg gcgtaacgga aagttggtag cttcttgcaa cttgctatga aactcttcga
     3121 tggtcaacgt tgagttcaca agccccaaaa ccagcgtacg gacgcgttcc ccgatctccg
     3181 gtgaaatgtc agagccaaat tgctgtaaag tcgtcaaaaa acgtttcaac ttgcttaact
     3241 ggcgggctcc acatgcagga ggcaagtgct gtgtggacag cgaggcagtg ctcgaagtgg
     3301 caggcccgtt cgaaaatcca ttcggggtac agggtgctcc attaatagcg gtgggggagt
     3361 gagacgagcc attcatcaat aaacacacca tcgaccactt caggcatcca tggaaacgtc
     3421 catgcggaag tgtcgcggga ccgtcttcgc gatgcgtgtg aggcgtgaaa cttgggggac
     3481 gcgttgcacc ctgactagcg gccggaggag gcggcggcat agacggcggt gtactgcggg
     3541 gctgagtctt aacctctgct ggcgaatctg gcatggcgga tgctttcgct ttacgatcca
     3601 ctggtgcggt gacgatggga tatgattgag ggcctggcac ggaacttccg atgtctgcgg
     3661 gggtcggaga tgccaggttc gctttcatgt cgtcaaggcc gccagcaagt gaggccatgg
     3721 cactgtattc tgtggtctgt tcgggtttga tcggttccgt agtcgtaaag atatcgctat
     3781 aatgttgacg ttcaaaaaca cggtcaagta cttcaagttg ctgttgcgtg aataaatcgc
     3841 cgcgcatctg tttgcgcaga aagtcacgtc ctggcaacga atgaccgttc ggcaccgggc
     3901 tttcctgaat gccctcatcg cgcttacgtt tgttggtatc ggcgcttggg gaggtaattc
     3961 ctaaaatccc cgagatgcta taggaagagc ctgcagagtc ggttgacacg ctggacacct
     4021 gagtgacgga acctgtactt acaatgctat gcgaagaagc gggaactggt tggttcgggg
     4081 gctgttgcac ctttgtacgg atgatacggt tgatgctaga cactgatggt acagtgtcgt
     4141 tatcgcagac acgctctgcc aataagcggt cgcggatttc ccacgcaaac atagtagggt
     4201 tttgacgctt gtactcagca attttttcaa cgaccttcgg cgtagctacc ttaggtttcg
     4261 agcctccgat aacccccggc ttaatacttc cggtttcata gtagcgcccc aggattttgg
     4321 atacacaacc gtgcgataca cgcagttgac gactaatatc acaggggcgt accccctgat
     4381 gtgccagttc cacaatacgt tgacgaacta cgtcaggcaa tggacggcca tttacaaaca
     4441 cccctcccag ttgattaaca cccccatgtc ctgtacggga agtgcgtgga gtggggtagt
     4501 ttttctccaa atccattccc cgggaagcgg ccgcacttcc cccggagcct ccgcttccgc
     4561 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     4621 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt
     4681 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     4741 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     4801 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     4861 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     4921 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     4981 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     5041 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     5101 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     5161 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     5221 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     5281 tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcgg ttcactaaac
     5341 gagcccggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     5401 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     5461 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     5521 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     5581 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     5641 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     5701 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     5761 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     5821 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     5881 atgggctatg aactaatgac cccgtaattg attactatta ataactagca cgcgttcgaa
     5941 gcttgagctc ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     6001 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     6061 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     6121 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     6181 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     6241 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     6301 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     6361 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     6421 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     6481 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     6541 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     6601 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     6661 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctta
     6721 tggtgtttta ctaaactttt ccatgttcta atcctcatcc tgtctacttg ccacacaatc
     6781 atcacctgcc atctgttttc cataatccct aatgatcttt gcttttcttc ttggcactac
     6841 ttttatgtca ctattatctt gtattactac tgccccttca cctttccaga ggagctttgc
     6901 tggtcctttc caaagtggat ttctgctgtc cctgtaataa acccgaaaat tttgaatttt
     6961 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     7021 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     7081 catttgtact gctgtcttaa gatgttcagc ctgatctctt acctgtccta taattttctt
     7141 taattcttta ttcatagatt ctactactcc ttgactttgg ggattgtagg gaatttccag
     7201 gtctgaagat cctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat
     7261 cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt
     7321 tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag
     7381 ctgtcccagt atttgtctac agccttctga tgtttctaac aggccagtat taactgcgaa
     7441 tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc
     7501 tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct
     7561 ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag
     7621 tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag
     7681 ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc
     7741 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     7801 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     7861 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa
     7921 agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg
     7981 cctcccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     8041 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     8101 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     8161 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     8221 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     8281 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     8341 taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg
     8401 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8461 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8521 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8581 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8641 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8701 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8761 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8821 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8881 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8941 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9001 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9061 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9121 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9181 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9241 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9301 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9361 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9421 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9481 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9541 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9601 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9661 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9721 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9781 cgataagtcg tgtcttaccg g
//
