LOCUS       sequence-407447-        9288 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_NOL4L from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_NOL4L
FEATURES             Location/Qualifiers
     source          1..9288
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             3724..3735
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(4049..4759,4760..4762,4763..4765))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4091..4112)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4440..4459
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4699..4720
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4762..4771
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4847..5050)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4876..4896)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5051..5354
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5611..5655)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5840..6073
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6433..6550
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7042..7167
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7214..7471
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7473..7852
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7883..7987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7988..8056,8057..8848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8206..8225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9019..9288,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagat caattttgtt
     1801 gcagaatcaa gttttctaac tcgtcagcag agcgcaataa gaacgcagca gactcacggt
     1861 aacccgcgat aagttggcgg acagcgctaa tttcagtggg agacagctgc gccgtaggaa
     1921 ctgggcgact agtgctagta ctcgacggag tcggcgttgg tgtagttgat gtagtagagg
     1981 ccccgccttt cattgataaa tctgtcgggc cattagaatg gtttcctgtt tgaagacttg
     2041 cgggaggttg cagattactg gacagtgcac cgtatccgcg atatgaataa ttcagtcctc
     2101 cgttggcgta cacggggtct tgtgaatacg aactggccgg aagagaatac gtgctgtgtg
     2161 tgattgcggc agaatcgcga gaatgttgct tatccagcgc gattggttcg tcctgcgatg
     2221 attggtagat ttccagacgc atacgtttcg cagctttgcg agtttcggac tcgcaggccg
     2281 cggccaaaat attttctgcc atcgcggagg ttaaatgcgg tggagtaggg cgggtcatct
     2341 ccatgccatt cttcttcata cggcgacaac tcttaaggta cgtgcgaatg cgtttgcgcg
     2401 cgcgttcctg gaactcaggg aattggcgcg aacaactttc aatgatggct tgaattttct
     2461 cctttggctg tttgctaatt gggaccatac ggtccagatt ctcgtctacg aataagcgga
     2521 cgaacatatt aaatgccttc aggcgttcgg ggtccatacc ctcagaatcg ttcattttgt
     2581 cattatcttc atggtcatca tggtcatcgt catcgtcgtc ggccgttgga gcacgtccaa
     2641 cggtcaaatc ttcggggcaa ccggacactt cagtcttaat gctgtcatag cttccgctcg
     2701 aataaggggg cgactctgga gtagtcttta cgccgtattt cacgcgggaa cgcaatccgt
     2761 ctgcgccaca accgtccgaa gggtagctgg ccgtccccaa agcagtcgct gggggaagtt
     2821 tgtcacacag attaattggc tgatcctccg ctgcagtagt gaaatccata ggtgccactg
     2881 cgccgtttcc gttcatctca ggaaatgctg gatcaccctg cgtcgaagaa gacgtggacg
     2941 gattcaaggt acttgaacca ttgcctgaac cagactctga ggaggagtcg tcgggaacca
     3001 ttcctgtcag agttggcgca gaataagatc cttggcccgc aggtggcaca tgcgggggat
     3061 accccggtaa tgtggtagat gcaaggtcac gaccagtgac aattgggtag gactggggac
     3121 caggaaccga agagccaata tcagcgggag tggggcttgc aaggttcgct ttcatatcgt
     3181 ctaagcctcc agctaaggac gccatcgctg agtattccgt agtttgttca ggcttgatag
     3241 gttcggtggt cgtgaagatg tcagagtagt gttgacgctc aaatacgcgg tccaagactt
     3301 ctaattgctg ttgtgtgaac agatctccgc gcatctgctt acgcaaaaag tcacgacccg
     3361 gaagactatg accgtttggc acaggactct cttgaatacc ttcatcacgt ttacgcttgt
     3421 tggtgtcggc ggaaggcgag gtgataccta agataccgga gatactgtat gatgaaccag
     3481 cggaatcagt ggacacacta cttacttggg ttactgaccc cgtgctaaca atactgtgag
     3541 atgaagcagg cactggttga ttgggcggtt gctgaacttt agtacgaata atacgattaa
     3601 tggaacttac ggatggaacg gtgtcattgt cacaaacacg ctctgccaac agacgatcac
     3661 ggatctccca tgcgaacatt gtggggtttt ggcgcttata ctcggcgatt ttttctacca
     3721 ctttgggagt tgcaaccttg ggcttactgc ccccaataac tcccggtttg atcgaaccag
     3781 tttcatagta gcgacccaga atctttgaca cacacccgtg agaaacacga agctgacgcg
     3841 aaatatcgca cgggcggact ccttgatgag ccagttcaac gatacgttga cgaacgacat
     3901 ccggcagagg acggccattt acgaacacac cgccaagctg attaacgccg ccatgtcctg
     3961 tgcgagatgt acgcggtgtg gggtaattct tttcaaggtc cattccccgg gaagcggccg
     4021 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     4081 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     4141 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     4201 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     4261 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     4321 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     4381 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     4441 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     4501 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     4561 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     4621 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     4681 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     4741 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     4801 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     4861 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     4921 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     4981 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     5041 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     5101 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     5161 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     5221 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     5281 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     5341 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     5401 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     5461 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     5521 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     5581 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     5641 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     5701 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     5761 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     5821 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     5881 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     5941 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     6001 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     6061 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     6121 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     6181 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     6241 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     6301 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     6361 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     6421 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     6481 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     6541 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     6601 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     6661 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     6721 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     6781 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     6841 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     6901 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     6961 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     7021 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     7081 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     7141 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     7201 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     7261 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     7321 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     7381 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     7441 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
     7501 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     7561 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     7621 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     7681 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     7741 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     7801 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
     7861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     7921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     7981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     8881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     8941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     9181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
