LOCUS       sequence-407449-        9312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PRKCE_AFTPH from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PRKCE_AFTPH
FEATURES             Location/Qualifiers
     source          1..9312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4073..4783,4784..4786,4787..4789))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4115..4136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4464..4483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4723..4744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4786..4795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4871..5074)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4900..4920)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5075..5378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5635..5679)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5864..6097
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6457..6574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7066..7191
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7238..7495
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7497..7876
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7907..8011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8012..8080,8081..8872)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8230..8249)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9043..9312,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatc
     1801 cgtccgcttt ttcctggcta cttgttgacg gtgtcaaagt tgcggggaac atcaacactt
     1861 tagcatgcat aaaggtcaaa tccggtaatc cagcaatgac tttaatggct tcctctgaaa
     1921 gatgctcttc gcgctttggc ttacgactcg tagacgtgct agtcttttcg accgtactca
     1981 taaggcgtgc aaaggcatcg gtgactttta aagacgatgt cgagatctcc agtttactag
     2041 tggttaattc ataaagttct gggtccactc cgtccagggg gttggtcaga cccgaagacg
     2101 accaatcgaa ttgaactggg ggcaatgatt cttgaaactg atcggaggtg catgtattca
     2161 tgtctggact catggtcgct gtttgcccaa tggaagcgat cttttccgcg gctgaaagtg
     2221 gctttaatgg ttccttggtc ggctctaaca tacctaagcc tgccgcatac ataggcacaa
     2281 tcactggttg cttcttgttt ccagtaaata aaatattgcg agtgtcgatg ccaagactcg
     2341 ataacagttt cttgttagaa tgtgaaccgc cccattggta gcgtaatcca tgtgcatcat
     2401 gaatgtcttg aagctccgtc cacacatcca acaactcccc ggattctgtc agagcctcac
     2461 gcgtcttaat gggcagagtg gaggtctcaa gtaagtgttt caacgatgtg acctcctcct
     2521 ctgcatcagg taccaggatc gagggaaagc atgcctcaaa gatacgctcc aaacgattta
     2581 acagtgcagt tttcgtttga aagcaacagt aaagttgagt aaggtacgga tgcttgcgtg
     2641 caagcgccaa gatgcgcttt tctgtcatgg tacaatcaac atcgtcatct tgcaagataa
     2701 catctttctt aagtacctta actgcataaa cctcatcttt gcctttcaac tcggcaagca
     2761 tcaccttacc gaaacttccc ttccccaata ccttaataaa gttaaattcg tctaacccca
     2821 gacgcttggc ctgcccctgg cgcacttcgc cgttttcacc tggggacatc aattgaccgt
     2881 ccggtgacga agctgcgcga tgttcttctc cacgattatc gaaggacagt gctttgcgga
     2941 tgttattttc caactctttg atctcctggt cacaaggcga ggtgggagca cttttgctac
     3001 gatcttcctc actgggggac gaacctgagg cgggttgggg actctccgcg ccagcgataa
     3061 gtttcttgcg acgctgacca gaattggtaa ttttgtctgg ggtgaccccc aagtcggcca
     3121 ataccttcgc gattccgcga gcatcgaccc cgcaatttgg tgccacattg gtttcgcagc
     3181 ggcgatgcac attcatctta cataccttgc actgtaatcc ctggcgtaat aagccccaca
     3241 gaagcgatcc gcagtgatcg caaaatgtag gaaccttgta gttgtgaata ccgaacttgt
     3301 gaggcatgtt aacgctgaag cgttgggagc ccacctgatc cggcgtttcc tgctttttaa
     3361 gaccagcgca ctttgtaata ataagctcat ggcaacgctt atgaaccaca caggtacaca
     3421 cctggcactg ataaccctgc ttgccaatta ctccccaaat aaaatcacga cagtgtgaac
     3481 aatacgtcgg ttggcgtaag tatgtggcca taaatttatg accgttgacc tgatgtacgc
     3541 ggcggcgaac agcgccttgg cgtttgcgtg gacgcatgcg ttcacgaaat acgcgctctt
     3601 cattgtcctt aggtgcctcc cccgaactac cgcttaagtc gatgatgacg tacacgcggc
     3661 cctccggctc aaggtcgatc caatcctcga agtggcgact accattctgc agcagctctt
     3721 cgaattgaat ggtgcagttt gccacaaaat catcgtaccc aattggggcg tcgtgaaaga
     3781 cggccaattc gattttgcgc ccgttacaaa catcggttac aaattcatca tgccaggccg
     3841 gtgagtttgt cttttgtttt gtggccgtct gaccaatgcg ggagtcatct acattaagtg
     3901 caatgtacgg atctaaaaga aaagtttggg gacgaggtcc aacggcgtga cgaagtgacc
     3961 acgccgtcgg ctttaatgat accgcctcac aaattttaat tttcaaaagg ccattgaaca
     4021 ctaccattcc ccgggaagcg gccgcacttc ccccggagcc tccgcttccg cccttgtaca
     4081 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     4141 cgcgcttctc gttggggtct ttgctcagct tggactgggt gctcaggtag tggttgtcgg
     4201 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4261 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     4321 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4381 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4441 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4501 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4561 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     4621 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     4681 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     4741 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     4801 gtggatcccg ggcccgcggt accgtcgact gcagaattcg gttcactaaa cgagcccggt
     4861 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     4921 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     4981 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     5041 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     5101 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     5161 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     5221 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     5281 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     5341 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     5401 gaactaatga ccccgtaatt gattactatt aataactagc acgcgttcga agcttgagct
     5461 cggtcccctc ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac
     5521 tctattcact atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat
     5581 cattatgaat aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact
     5641 tgcccattta tctaattcca ataattcttg ttcattcttt tcttgctggt tttgcgattc
     5701 ttcaattaag gagtgtatta agcttgtgta attgttaatt tctctgtccc actccatcca
     5761 ggtcgtgtga ttccaaatct gttccagaga tttattactc caactagcat tccaaggcac
     5821 agcagtggtg caaatgagtt ttccagagca accccaaatc cccaggagct gttgatcctt
     5881 taggtatctt tccacagcca ggattcttgc ctggagctgc ttgatgcccc agactgtgag
     5941 ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa ttgttctgct gctgcactat
     6001 accagacaat aattgtctgg cctgtaccgt cagcgtcatt gacgctgcgc ccatagtgct
     6061 tcctgctgct cccaagaacc caaggaacaa agctcctatt cccactgctc ttttttctct
     6121 ctgcaccact cttctctttg ccttggtggg tgctactcct aatggttcaa tttttactac
     6181 tttatattta tataattcac ttctccaatt gtccctcata tctcctcctt atggtgtttt
     6241 actaaacttt tccatgttct aatcctcatc ctgtctactt gccacacaat catcacctgc
     6301 catctgtttt ccataatccc taatgatctt tgcttttctt cttggcacta cttttatgtc
     6361 actattatct tgtattacta ctgccccttc acctttccag aggagctttg ctggtccttt
     6421 ccaaagtgga tttctgctgt ccctgtaata aacccgaaaa ttttgaattt ttgtaatttg
     6481 tttttgtaat tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc
     6541 actgtacccc ccaatccccc cttttctttt aaaattgtgg atgaatactg ccatttgtac
     6601 tgctgtctta agatgttcag cctgatctct tacctgtcct ataattttct ttaattcttt
     6661 attcatagat tctactactc cttgactttg gggattgtag ggaatttcca ggtctgaaga
     6721 tcctgctgct tgctgtgctt ttttcttact tttgttttgc tcttcctcta tcttgtctaa
     6781 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     6841 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     6901 tatttgtcta cagccttctg atgtttctaa caggccagta ttaactgcga atcgttctag
     6961 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     7021 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     7081 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     7141 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     7201 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     7261 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     7321 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     7381 agctcccagg ctcagatctg gtctaaccag agagacccag tacaggcaaa aagcagctgc
     7441 ttatatgcag gatctgaggg ctcgccactc cccagtcccg cccaggccac gcctcccatg
     7501 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg
     7561 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt
     7621 ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat
     7681 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc
     7741 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg
     7801 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta
     7861 gtcaataatc aatgtcgacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7921 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7981 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8041 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8101 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8161 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8221 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8281 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8341 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8401 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8461 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8521 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8581 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8641 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8701 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8761 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8821 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8881 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8941 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9001 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9061 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9121 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9181 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9241 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9301 gtgtcttacc gg
//
