LOCUS       sequence-407456-       10983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SEC22B_BCL9 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10983)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SEC22B_BCL9
FEATURES             Location/Qualifiers
     source          1..10983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(114..218)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        249..628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             630..887
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    934..1059
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1551..1668
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2028..2261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2446..2490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2747..3050
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3051..3254
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3205..3225
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3330..3339
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3336..3338,3339..3341,3342..4052)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3381..3402)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3642..3661)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3989..4010
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(8249..8268)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8314..8369
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8368..8387)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8730..8746)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8730..8746)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8743..8765)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8754..8770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8778..8784,8785..8802,8803..8808))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8823..8844
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8903..9098
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8949..9084
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9104..9238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9141..9160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9195..9214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9306..9323)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9477..10065)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9557..9576)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10236..10983,1..44,45..113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10859..10878
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
       61 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      121 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      181 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
      241 cctgggtcga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
      301 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      361 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      421 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      481 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      541 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      601 ctacgtatta gtcatcgcta ttaccatggg aggcgtggcc tgggcgggac tggggagtgg
      661 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
      721 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      781 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      841 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
      901 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
      961 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     1021 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     1081 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     1141 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatactgg cctgttagaa
     1201 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     1261 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     1321 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     1381 aaaaaagcac agcaagcagc aggatcttca gacctggaaa ttccctacaa tccccaaagt
     1441 caaggagtag tagaatctat gaataaagaa ttaaagaaaa ttataggaca ggtaagagat
     1501 caggctgaac atcttaagac agcagtacaa atggcagtat tcatccacaa ttttaaaaga
     1561 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     1621 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     1681 agggacagca gaaatccact ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg
     1741 gcagtagtaa tacaagataa tagtgacata aaagtagtgc caagaagaaa agcaaagatc
     1801 attagggatt atggaaaaca gatggcaggt gatgattgtg tggcaagtag acaggatgag
     1861 gattagaaca tggaaaagtt tagtaaaaca ccataaggag gagatatgag ggacaattgg
     1921 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1981 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     2041 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     2101 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     2161 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     2221 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     2281 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     2341 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     2401 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     2461 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     2521 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     2581 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2641 cacctcccaa ccccgagggg accgagctca agcttcgaac gcgtgctagt tattaatagt
     2701 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2761 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2821 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2881 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2941 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     3001 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     3061 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     3121 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     3181 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     3241 atataagcag agctcgttta gtgaaccggg ctcgtttagt gaaccgaatt ctgcagtcga
     3301 cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     3361 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     3421 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     3481 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     3541 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     3601 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     3661 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     3721 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     3781 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     3841 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3901 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     3961 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     4021 ggatcactct cggcatggac gagctgtaca agggcggaag cggaggctcc gggggaagtg
     4081 cggccgcttc ccggatggtg ttattaacta tgattgctcg cgtcgctgat gggttaccgc
     4141 tggcagcgag catgcaagaa gatgaacaga gtggtcgtga tttacagcag tatcagtccc
     4201 aggccaaaca gctttttcgt aaacttaacg agcagtcccc aacacgttgt acacttgaag
     4261 ctggggcgat gaccttccag gccgcagaag ctgtacttaa agggcaagtg gagacgattg
     4321 tatcgtttca tatccagaat attagtaata ataaaactga acgttcaacc gcaccgctta
     4381 acactcagat ctcggcatta cgcaatgatc ctaagccatt gccgcaacag ccgccggctc
     4441 cggctaatca agaccaaaac tccagtcaaa atactcgttt acagcctaca ccccccattc
     4501 cggcgcctgc ccctaaacca gcggctcctc cgcgcccact ggaccgcgag agtcccggag
     4561 ttgaaaataa attgatcccc agcgttgggt ctcctgcctc ctctacccca ttgccaccag
     4621 acggtaccgg cccaaatagc actccaaata atcgcgctgt gacaccagtg agccaaggaa
     4681 gcaatagttc cagtgcagac ccaaaagcac ccccgccccc tcccgtatca tctggggaac
     4741 caccgacgtt gggtgaaaat ccggatggcc tttcacaaga acagctggag catcgtgagc
     4801 gttcactgca gaccttgcgc gatattcaac gcatgttatt ccccgacgaa aaggagttta
     4861 ccggagcaca gtctggtggc ccccagcaaa acccgggcgt actggatggc cctcaaaaga
     4921 agccggaggg cccgattcag gccatgatgg ctcagtctca gtccttaggg aagggtccag
     4981 gtccacgcac tgacgtgggt gcccctttcg ggccccaggg gcatcgtgac gttccgtttt
     5041 cgcccgacga gatggtgcct ccatccatga acagccaatc cggtacgatc ggtcctgacc
     5101 atttggacca catgaccccc gagcagattg catggcttaa attgcagcaa gagttctacg
     5161 aggagaagcg tcgcaagcaa gaacaggtag ttgtgcaaca gtgctcactg caggacatga
     5221 tggtccacca acatgggccg cgtggtgtgg tccgtggacc ccctcctccg tatcagatga
     5281 cgcccagtga aggatgggca ccagggggca cggagccgtt tagtgatggc atcaacatgc
     5341 cacattctct gccgccacgt ggcatggctc ctcaccccaa catgccgggg tcgcagatgc
     5401 gcttgcccgg tttcgctgga atgattaact cggaaatgga gggcccgaac gttccgaacc
     5461 ccgcctcccg cccaggattg tccggggtta gctggccaga cgacgtgccg aaaattcctg
     5521 acggccgcaa cttcccccca gggcaaggca ttttctcggg gccagggcgt ggggagcgct
     5581 ttcccaatcc acagggtctt tcggaggaaa tgttccagca acagcttgcc gaaaaacaac
     5641 ttggactgcc acctggaatg gcaatggaag gaattcgtcc tagcatggag atgaaccgca
     5701 tgattcccgg atcccagcgc cacatggagc ctggcaacaa tccgatcttc ccccgcatcc
     5761 ccgttgaagg gccactgtcg ccctctcgcg gcgattttcc taagggaatc cctccccaga
     5821 tgggccccgg ccgtgaattg gagtttggta tggtaccgtc tggcatgaaa ggcgatgtaa
     5881 atttgaatgt caatatgggg agcaattctc aaatgattcc ccagaagatg cgtgaagccg
     5941 gtgccggacc tgaggaaatg ttgaaactgc gcccaggtgg gtcagatatg ctgcctgcac
     6001 agcaaaagat ggtccctctt ccgtttggag aacatccgca acaagagtac ggtatgggac
     6061 cgcgcccctt cttaccgatg tcgcaagggc ccggtagtaa ttctgggtta cgcaatttac
     6121 gcgagccgat cgggccggac caacgcacaa actcacgcct ttcacacatg ccgcccttac
     6181 ctttgaatcc tagttccaat ccaacatctc tgaatactgc accgcctgtg cagcgtggtt
     6241 tgggccgtaa gccattggat atcagcgtcg cgggctcaca ggtacactca cccgggatca
     6301 acccgcttaa aagtcccaca atgcaccagg tacagtcgcc aatgttagga tctccaagcg
     6361 gaaacttaaa atctccccag acgccctcgc aactggccgg gatgttggcc ggaccggcag
     6421 ctgctgctag catcaagagc ccacctgttt taggatcggc tgccgcgtct ccagtccact
     6481 tgaaatcacc aagtttgccc gcgccatcac cgggctggac aagctctccg aaacctcccc
     6541 ttcaatcccc tgggattccc ccaaatcaca aggctccctt aactatggcc tcgccggcga
     6601 tgcttggcaa tgttgaatct ggcggaccac cgcctccgac agcatcccag ccggcaagtg
     6661 tcaacatccc aggatcactg ccttcttcta cgccctatac gatgccgcct gagccgactt
     6721 tgtctcaaaa tcccttaagt atcatgatga gccgcatgtc aaaattcgcc atgccttcca
     6781 gcactcctct gtatcatgat gctattaaga ctgttgcttc ctccgacgat gactcccctc
     6841 cagcccgttc accaaattta cccagcatga ataatatgcc gggaatgggt atcaatactc
     6901 agaatcctcg catcagtgga ccaaaccctg tcgtcccaat gccgactctg agccctatgg
     6961 ggatgacaca accattgtcc cactcgaatc aaatgccctc accgaatgct gttggaccga
     7021 acatccctcc acacggcgtg cctatgggcc cgggtttaat gagtcacaac ccaattatgg
     7081 ggcacggtag tcaagaacca ccgatggttc cacaaggtcg catgggcttc ccgcaaggat
     7141 tccctccagt gcaatcaccg ccccaacagg tacctttccc ccataacggt cccagcgggg
     7201 gtcagggtag ttttcctggc ggcatgggtt tccccgggga gggtccttta ggccgtccta
     7261 gcaatttgcc tcagtcaagt gccgatgctg ctctttgcaa accgggaggc ccggggggtc
     7321 ctgatagctt tactgtatta ggcaactcca tgccttcggt attcactgat cccgatcttc
     7381 aagaggtaat ccgtcccggg gcaacaggga ttccggaatt tgacctttct cgcatcatcc
     7441 ccagtgaaaa acccagtcaa acgttacagt attttccccg tggtgaggtt ccgggacgta
     7501 aacaacctca gggacccggc ccagggttct ctcacatgca aggcatgatg ggtgaacagg
     7561 cgccacgcat ggggctggcc ttaccgggca tgggagggcc gggtccggtt gggacaccgg
     7621 atatcccttt aggaacagct ccgtctatgc ctgggcataa cccaatgcgc ccccctgcct
     7681 ttttgcaaca aggtatgatg ggcccgcacc atcgtatgat gtctcctgcg cagagtacaa
     7741 tgccgggaca accaactttg atgtcaaatc ccgccgccgc agtcgggatg atcccgggaa
     7801 aggaccgtgg accggcaggc ctgtacaccc accctggtcc tgtgggctct ccaggcatga
     7861 tgatgtctat gcaggggatg atgggtcccc agcagaatat catgattccg ccgcaaatgc
     7921 gccctcgtgg catggccgct gacgtcggta tgggaggatt tagccaaggc ccaggaaacc
     7981 ctggcaatat gatgttttga accggttcta gataagatta cggtaccttt aagaccaatg
     8041 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     8101 ctaattcact cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta
     8161 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8221 taaagcttca gctgctcgag ctagcagatc tttttccctc tgccaaaaat tatggggaca
     8281 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     8341 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     8401 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     8461 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     8521 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     8581 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     8641 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     8701 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     8761 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8821 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8881 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
     8941 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     9001 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     9061 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
     9121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     9181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
     9241 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9301 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     9361 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     9421 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     9481 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     9541 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     9601 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     9661 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     9721 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     9781 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     9841 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     9901 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     9961 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10021 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10081 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10141 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10201 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10261 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    10321 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    10381 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    10441 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    10501 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    10561 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    10621 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    10681 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    10741 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    10801 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    10861 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    10921 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    10981 ccc
//
