LOCUS       sequence-407459-        9276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, RUNX1_CBFA2T3 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_RUNX1_CBFA2T3
FEATURES             Location/Qualifiers
     source          1..9276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4037..4747,4748..4750,4751..4753))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4079..4100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4428..4447
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4687..4708
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4750..4759
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4835..5038)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4864..4884)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5039..5342
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5599..5643)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5828..6061
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6421..6538
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7030..7155
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7202..7459
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7461..7840
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7871..7975
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7976..8044,8045..8836)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8194..8213)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9007..9276,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gcttctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 gtggtacggt gtccaaaggt cccggtggtg aaggggaacc tggacggctg ggaccagcag
     1861 accctgcttc ggagggacta gccgctccga caggcaatga gggccccaac gaatgagcgg
     1921 cttctggcgg accaggtacc gggtcagcaa caacggcggt agggccctgc aatgattgac
     1981 cacacacgtg gtggtgcttc tcccaatcgc gatgctgaca gaagctgccg caatagcgag
     2041 cagcattgca acccgagcac gtttcactgg ctttacgtcc acagttccaa cacgactcac
     2101 tggagtcttc ctgttgattg ataacagtaa gagcgtcttc actcgcttga cgtttagctt
     2161 cggcaagtgc acgttccatt ttggcgcgct ctgttgtgat taattcgtgg gccttacgtt
     2221 cggcgtcgct aaccgccttc tgcaattcac tcatggcctg gcgtttaacc tcatttactg
     2281 cctcttccgc cttgcgccaa atatcttccg gaacgtaccc ggtaagcgtg cgtggtaaaa
     2341 actcgcgtgg gacgtccagt tgaggaccct ccggcccggc agatgacgag cgcgggcgtg
     2401 cagcagctgg tgccggcccc ttttttgtat cctccgcgtc cgaatagcgg cgcgcccaat
     2461 ggtttaattc ctcacggtct gcttcctgac aacgacgaag cacagttaat gaacgacgag
     2521 ttttttcgac catatccata atgcagttca aaagattgtt aaggtgcttc cattcttcag
     2581 cccactcgcg ttctgttaac ttatgatcga ttacttcttc ctggcgggac ccaggtacca
     2641 ctaacgggcg atggcgctcg cgcaattcac gggggtccgg gtgacgatac gcgtcacgaa
     2701 agtggtgcgc catagcgatg tcctccaaac gataatgagg tggtggcgtc ggctgcgggg
     2761 gtccattgga aggggagtag cgttgagctg gatttaaagt gcaggggcgc tttgacaaat
     2821 gctcgggatg cagagggtcg cgatcagatc cgttctcctt agtacggtcc ggagtacggc
     2881 gtttaccatt ctcatttact tccaaaagta attccgagga atcaatagga ctggacgcac
     2941 tggcatccag taacaactgt tcgtgctgtg ccaagtattg ggccggggtc tgcttagcca
     3001 agcgtgcgca gtgaagaagt tcgcgctgaa gtaatggtaa gttagctttt aagaagggga
     3061 taacaaaagg gcgtaacgga aagttggtag cttcttgcaa cttgctatga aactcttcga
     3121 tggtcaacgt tgagttcaca agccccaaaa ccagcgtacg gacgcgttcc ccgatctccg
     3181 gtgaaatgtc agagccaaat tgctgtaaag tcgtcaaaaa acgtttcaac ttgcttaact
     3241 ggcgggctcc acatgcagga ggcaagtgct gtgtggacag cgaggcagtg ctcgaagtgg
     3301 caggcccgtt cgaaaatcca ttcggggtac agggtgctcc attaatagcg gtgggggagt
     3361 gagacgagcc attcatcata cgcggctcac gtgggccgtc aacggtgatc ttgattgcgc
     3421 gatgatatgt tgccacttgc ggcgggtttg taaagactgt gatggttaag gtgaatgatt
     3481 tcccacgccc actgcgaccc acgaaacgca gatcgttgaa acgtgcaacc tgattcttca
     3541 ttgctgcggt agcgttacgc aactccgcac tgtaattttc atcgttgcct gccatcactg
     3601 ttaccaaagt accgtcaggc acatcaccca gcgctacaac cttgaacgcg attggcaagg
     3661 ttttattgca gcgccagtgt gtgggtaaca cactgcataa aaaattagga ctatccgtac
     3721 gtacaagctc accgggatgg tccgccagaa cttcaaccat actacgatcc ccgctacgca
     3781 gtttgccggc cagtgcagca ccggcgtcgg gtgcgccaag gggcaacgct tccgacattt
     3841 tgcccggaga caaggcggtc gagggagggg taaagcggcg cgaagtactc gcatcgtgca
     3901 cgtcacgcga cgggttcatt cctaaaatgc actcgcgcat gaagcattgg ggatatgaag
     3961 gaaaagattc gaagatacta tctgaggcca ttccccggga agcggccgca cttcccccgg
     4021 agcctccgct tccgcccttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca
     4081 cgaactccag caggaccatg tgatcgcgct tctcgttggg gtctttgctc agcttggact
     4141 gggtgctcag gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct
     4201 gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt
     4261 tcaccttgat gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt
     4321 tgtactccag cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct
     4381 cgatgcggtt caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc
     4441 cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga
     4501 agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa gcactgcacg ccgtaggtca
     4561 gggtggtcac gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca
     4621 gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc ggacacgctg aacttgtggc
     4681 cgtttacgtc gccgtccagc tcgaccagga tgggcaccac cccggtgaac agctcctcgc
     4741 ccttgctcac catggtggcg accggtggat cccgggcccg cggtaccgtc gactgcagaa
     4801 ttcggttcac taaacgagcc cggttcacta aacgagctct gcttatatag acctcccacc
     4861 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     4921 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     4981 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     5041 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     5101 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     5161 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     5221 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     5281 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     5341 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     5401 tagcacgcgt tcgaagcttg agctcggtcc cctcggggtt gggaggtggg tctgaaacga
     5461 taatggtgaa tatccctgcc taactctatt cactatagaa agtacagcaa aaactattct
     5521 taaacctacc aagcctccta ctatcattat gaataatttt atataccaca gccaatttgt
     5581 tatgttaaac caattccaca aacttgccca tttatctaat tccaataatt cttgttcatt
     5641 cttttcttgc tggttttgcg attcttcaat taaggagtgt attaagcttg tgtaattgtt
     5701 aatttctctg tcccactcca tccaggtcgt gtgattccaa atctgttcca gagatttatt
     5761 actccaacta gcattccaag gcacagcagt ggtgcaaatg agttttccag agcaacccca
     5821 aatccccagg agctgttgat cctttaggta tctttccaca gccaggattc ttgcctggag
     5881 ctgcttgatg ccccagactg tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag
     5941 caaattgttc tgctgctgca ctataccaga caataattgt ctggcctgta ccgtcagcgt
     6001 cattgacgct gcgcccatag tgcttcctgc tgctcccaag aacccaagga acaaagctcc
     6061 tattcccact gctctttttt ctctctgcac cactcttctc tttgccttgg tgggtgctac
     6121 tcctaatggt tcaattttta ctactttata tttatataat tcacttctcc aattgtccct
     6181 catatctcct ccttatggtg ttttactaaa cttttccatg ttctaatcct catcctgtct
     6241 acttgccaca caatcatcac ctgccatctg ttttccataa tccctaatga tctttgcttt
     6301 tcttcttggc actactttta tgtcactatt atcttgtatt actactgccc cttcaccttt
     6361 ccagaggagc tttgctggtc ctttccaaag tggatttctg ctgtccctgt aataaacccg
     6421 aaaattttga atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat
     6481 tatgtctact attctttccc ctgcactgta ccccccaatc cccccttttc ttttaaaatt
     6541 gtggatgaat actgccattt gtactgctgt cttaagatgt tcagcctgat ctcttacctg
     6601 tcctataatt ttctttaatt ctttattcat agattctact actccttgac tttggggatt
     6661 gtagggaatt tccaggtctg aagatcctgc tgcttgctgt gcttttttct tacttttgtt
     6721 ttgctcttcc tctatcttgt ctaaagcttc cttggtgtct tttatctcta tcctttgatg
     6781 cacacaatag agggttgcta ctgtattata taatgatcta agttcttctg atcctgtctg
     6841 aagggatggt tgtagctgtc ccagtatttg tctacagcct tctgatgttt ctaacaggcc
     6901 agtattaact gcgaatcgtt ctagctccct gcttgcccat actatatgtt ttaatttata
     6961 ttttttcttt ccccctggcc ttaaccgaat tttttcccat cgcgatctaa ttctcccccg
     7021 cttaatactg acgctctcgc acccatctct ctccttctag cctccgctag tcaaaatttt
     7081 tggcgtactc accagtcgcc gcccctcgcc tcttgccgtg cgcgcttcag caagccgagt
     7141 cctgcgtcga gagagctcct ctggtttccc tttcgctttc aagtccctgt tcgggcgcca
     7201 ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca
     7261 cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc
     7321 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
     7381 ccagtacagg caaaaagcag ctgcttatat gcaggatctg agggctcgcc actccccagt
     7441 cccgcccagg ccacgcctcc catggtaata gcgatgacta atacgtagat gtactgccaa
     7501 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
     7561 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
     7621 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
     7681 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
     7741 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
     7801 gtaattgatt actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg
     7861 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7921 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7981 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8041 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8101 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8161 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8221 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8281 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8341 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     8401 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     8461 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     8521 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     8581 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     8641 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     8701 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8761 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8821 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8881 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8941 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9001 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9061 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9121 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9181 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9241 ggctgctgcc agtggcgata agtcgtgtct taccgg
//
