LOCUS       sequence-407461-        9678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SEMA6C_DLG2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9678)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SEMA6C_DLG2
FEATURES             Location/Qualifiers
     source          1..9678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4439..5149,5150..5152,5153..5155))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4481..4502)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4830..4849
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5089..5110
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5152..5161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5237..5440)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5266..5286)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5441..5744
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6001..6045)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6230..6463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6823..6940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7432..7557
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7604..7861
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7863..8242
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8273..8377
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8378..8446,8447..9238)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8596..8615)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9409..9678,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaaa
     1801 gtttctcctt ggacggaatc caaatgaacg gtcctgattg ttcttcgatc accaacttac
     1861 actgattgta aatatcttct aacgtgtctc cctgaacaat tgcggtaaag tattcaccaa
     1921 actcctgttc cagcttgatc gcgcgatcgt aggttttctt cgcttgctcc tcagtcagac
     1981 gcttgttcat ttccattaaa ggctccaagc tgcgaggctt aatgaagatt gcaatcggat
     2041 acaactgagc aacttgaaga cgtttaatag cgttgccact cacgtctaaa atacagtgct
     2101 tcccacgctc agccacgaaa cgaactgatt gaacggatgt tccgtataaa ttgtcattgt
     2161 actgaccggc ttcaataaat ttgtgctctt gaatatcttt ctccatctgc tcacgcgaga
     2221 tgacgaagtg gtagtcgcgt ccgtctacct cataatcacg cttaggacgg gtggtgtgag
     2281 ggacacacga gccgaattta tcaggaaatt cggaaatcag gtcgtcattg atgcgatcct
     2341 tcatcgggcc caggatgata acagggcggg tgtagttgat ttcttggcgc gtgacaggtt
     2401 catacgaaag gatcaaatct tcctgaccac gaagagtctt agttccatac ccgtcgtcac
     2461 ctaagcctgg aatatctcct ttcgaatcaa tcacccccgg cttggcgtta aattttacag
     2521 ttttcaaacg agcgcgttct ttgcgctcta cgcgacgttt agatggaatg acacccatct
     2581 cttcactgtc cccctctaac atgacacggc gcgcctgcca ccattcatcg tccgatgcat
     2641 taataacgtg cagaatgtcg ccatatttaa aagacaatcc ttgggaaggc aaaccgctat
     2701 cctttgactt gtcgtaatca aacatagcgc gtacataaag agagcgtttt tgattcgtac
     2761 gtaaactgcc ggacccggag gacatagagt ggttcatcat ctgttcacgc aagtcgtgaa
     2821 ttttggcctc gaagcgtgcg taatcttctg gctggtattg tgcaataatg gtaaccgtct
     2881 gcccagcccc tttaagcgca gctgccgctt gctcatgtga ggcaccacgc aaatcaatac
     2941 cgttgaccga caaaatttgg tcaccacgtt gcaattcgcc gctcaagtcc gcaggcccac
     3001 cagcaagaat gaatgatacg aagatgcctt ctccgtcctc tccaccaacg atattgaatc
     3061 ccaaccccgt tgaaccttta tgtaatacga ccttgcgcgg ctccccctcc agggagacgg
     3121 cacgctgtaa ggtcatggaa ggctggcgag ttgcggtact gtgctgagag tgtgaagtca
     3181 tttcactatc aggcagcaat cccaggtggt aatgcgagta aggtgcgctc aacaggaagg
     3241 atttatcaca ttccaccggg gagtagtgac gtggcgacgc tggcttatcg caaagtttat
     3301 ttacagtcga atagactggt tctggtgggc gggtatagtc gtcgtcaacc agcatgtgtt
     3361 taggaattgg gctataacgc cccggagaaa tagggggcaa cgaagtcttg tactcaagcg
     3421 ttccgttgtt accactaagc aggtgattct ccatgggtgg agaatatgaa tgggtaatgt
     3481 cgggtggccc gtaggggtcg gtcatgtaaa tcgttgtagg cttgccaacc ttaagataaa
     3541 caacctcgga tgtgtttttc aagatagcca ccgcttcctc atgggtgacc tcttctaaac
     3601 tatagttatt gaccataaga aggcgatcgc cgacctgtaa gcgtccatct ttttgagccg
     3661 caccaccatc aataatcttt gtaacgtaaa tactgttatc accaggaatg tgctggttcc
     3721 ccaccccgcc cgcaatcgaa aatcccaagc ctttcggacc cttaaacaat ttaatctcta
     3781 ctacggtttc caaaatggga cggcgacggc gcacatacaa gcggacgatg gagcctgctt
     3841 ccttcaaggc ctccactgct ttagagtggc taacctcact cacatctact tcattgacgc
     3901 gcaggatgca atcattcaca cgaaggcgcc cgtcctcggc tgcagcgccg cctgggatga
     3961 tcttagtaat gaaaatgcct gggtcatccc caatatgagg gttgtccgtt cctcctgcga
     4021 tcgaaaatcc aaggccacta ttgccacgtt caagtgtaat ctcttcaaat tcatactcaa
     4081 tctcggtgcc atttaccttg ttaggtacta aaccctcacc ctcttcttct gcctgaaggt
     4141 cgaacgagaa cacgtgatca cgtgcggcaa ccaacagcgt acggttcaaa gtcagaaaac
     4201 gttggaaatc cagacccaat tcggcggcga ccgcgtcatc ttctaagcca cggaaccacg
     4261 acaaggggga cgtaccttgc aagtccgaga taagcaacgg aagtgggtcc tgagggaatg
     4321 cggcttgagt atgtggcaga gacagcaaca acaataaaag caaaagaggc atgaaatgcg
     4381 gggcacgggg cattccccgg gaagcggccg cacttccccc ggagcctccg cttccgccct
     4441 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4501 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     4561 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4621 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     4681 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     4741 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4801 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4861 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4921 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     4981 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     5041 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     5101 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     5161 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcggttc actaaacgag
     5221 cccggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     5281 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     5341 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     5401 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     5461 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5521 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5581 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5641 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5701 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5761 ggctatgaac taatgacccc gtaattgatt actattaata actagcacgc gttcgaagct
     5821 tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     5881 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     5941 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     6001 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     6061 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     6121 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     6181 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     6241 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     6301 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     6361 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     6421 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     6481 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     6541 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctccttatgg
     6601 tgttttacta aacttttcca tgttctaatc ctcatcctgt ctacttgcca cacaatcatc
     6661 acctgccatc tgttttccat aatccctaat gatctttgct tttcttcttg gcactacttt
     6721 tatgtcacta ttatcttgta ttactactgc cccttcacct ttccagagga gctttgctgg
     6781 tcctttccaa agtggatttc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     6841 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     6901 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     6961 ttgtactgct gtcttaagat gttcagcctg atctcttacc tgtcctataa ttttctttaa
     7021 ttctttattc atagattcta ctactccttg actttgggga ttgtagggaa tttccaggtc
     7081 tgaagatcct gctgcttgct gtgctttttt cttacttttg ttttgctctt cctctatctt
     7141 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     7201 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     7261 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccagtattaa ctgcgaatcg
     7321 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     7381 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     7441 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     7501 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     7561 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     7621 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     7681 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     7741 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     7801 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     7861 cccatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     7921 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     7981 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     8041 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     8101 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     8161 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     8221 taactagtca ataatcaatg tcgacccagg tggcactttt cggggaaatg tgcgcggaac
     8281 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8341 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8401 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8461 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8521 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8581 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8641 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8701 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8761 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8821 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8881 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8941 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     9001 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     9061 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9121 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9181 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9241 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9301 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9361 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9421 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9481 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9541 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9601 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9661 taagtcgtgt cttaccgg
//
