LOCUS       sequence-407466-        9474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PML_RARA from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9474)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PML_RARA
FEATURES             Location/Qualifiers
     source          1..9474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4235..4945,4946..4948,4949..4951))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4277..4298)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4626..4645
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4885..4906
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4948..4957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5033..5236)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5062..5082)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5237..5540
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5797..5841)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6026..6259
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6619..6736
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7228..7353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7400..7657
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7659..8038
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8069..8173
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8174..8242,8243..9034)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8392..8411)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9205..9474,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagg
     1801 gtgagtgcgt tgccggagag ctgcgatttg atgacggaga cagagaaggt gaacaagagc
     1861 cggggggcgg ggctaatcct cccccatcgc gaccaccccc tcctggctgg ccagacaggg
     1921 tgtcaagccc ttcgctattc tctaacattt cttgaatcaa gggaggcatg cttccaggaa
     1981 tctccatttt aagagtaatt acacgctcgg ctcctttcgc tgagattgaa cgcaggtcag
     2041 tgattttcat caacattttg gggaacatgt gtggacggga cgggcgacgt ttacgaacat
     2101 agaccttaag tgcctcaagc aaaggctctt gcagcatatc cacgcgatcg ggttgctcaa
     2161 gatcctggcg atcgccgcaa atcaaacaaa tcgcggacaa cagccctgtc tctgcatcgt
     2221 ccatttccag gggcaacaac tgatttgcaa aggcaaagac aagatcagtc aatgggccaa
     2281 atcctgcatt gtgcatttgc gtgcggttaa gcgttaagcc atcggagaat gtcattgtat
     2341 cttgctccgg cgtataacgc gtgcagatgc gcaaaatcag aatatcaaga caggcagctt
     2401 taagcaaagt gatttggtcc gcaattgtca aagtcgtgaa gcctggcaac tgcttcgcaa
     2461 attcaacggt cttgatgata catttcgtgg ataactccga aaatttatcc cacaaatcaa
     2521 tgtctaacga gacgcgttgt tcggatgagt tattcgtggt atacttacca agttgacaca
     2581 aagcggggaa cgtttcttgg tgtgctttgc gcactttctc aattaactct cctacctcag
     2641 gagtaagtgt atagctctct gagcattctg gtttaggtac ctcttttttt ttcttgttac
     2701 ggtcattacg aacactctcc tttgacatcc ccacctcaaa acacttctga aggcggcagt
     2761 attgacagcg attacgagta accttattaa tgatacaatt tttgtcgcga tgacaggtat
     2821 aaaccatatt tttttggatg gagcgacgga agaagccctt gcagccctca cacgctgaga
     2881 caccataatg gtagccagaa cttttgtcct ggcagacaaa acacggcttg taaatgcggg
     2941 gcagtggcgg cggtgagggt ggtgaaggta caatctcttc tgatgatgaa ctctgcgtct
     3001 cgatcgcctt cccttgtgta atgcaagaac taagatcttg aagacgtacc ttaaattcat
     3061 cgaagccatc agtacgaacg gcggcctgaa ggctctgcgg ctcctcctgg cgaaggcgac
     3121 ataacgcttg acgcaaaaac ccgtgcatgt ctaatacctc ctgatccgag gcataacatt
     3181 tcatacgctg aactaaggct gatcctgtac gaatgcgttg cagtaccgcg tccaaacgcc
     3241 ctaagcggga tgccatctcc tcgtaatcgc gttggtagcg agcatccacc gcttccaaca
     3301 attcgcgttc ttgcgcacga acgtgagcta ctacctgacg tacgcgctca cgaatcaact
     3361 cctcagtctc agcgcgtgca cggcccaatt gcccaacagc agcgtgcatc tgtgcgtgca
     3421 ctgctccaaa cgctgaatcc tgctcctgca aagcctgagt catggcgtca agttcttcct
     3481 gacgttgctg gatttccgcc gaaatgtcgc actttaattc tgaatgcgac gagtccagca
     3541 atgcgcagct acaacaaagt ggtttcgagc acccacggca ataaatagat gttaaggtag
     3601 gagtacggtg gttagggtta gagcaaaaga tgttattagt tttgcgtgtt ccgtctaaaa
     3661 actcgcgcac agattggtta cgcagctctg ccagtgggcg agcctcgtgt ttaaggaacc
     3721 attgatgcgc ttcaaagcac ttagcgcaaa gtaattgctc gcattcaaaa caccaaaaat
     3781 cggcagattc tttgcagcgg gtgcacactg cttgcgcgtc cacaatttgg cgatatacag
     3841 acaggcgacg ttgcaggctc tcaaaaaaca cattatccag cgccggcgta tctgcaccaa
     3901 gcggccaagg cgcttggcag attggacact gcatacccga ggcttccaag caaccgctac
     3961 acagggtgtg caaacatggc aacaacttag ggcacttggc ctccgcctga cactgctggc
     4021 aacgaaggaa ctgaaattcc tcctcggacg cgggggcacg ctctgtaggg ctcgggctcg
     4081 gacttggttg acgaccctca ctgggtgttt ctggcggtgg catcgtcggc tcctgtggac
     4141 gagcaggatc ttgttgtgga cgaggtgaac gtgctggtgc aggctccatt ccccgggaag
     4201 cggccgcact tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag
     4261 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     4321 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     4381 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     4441 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     4501 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     4561 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     4621 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     4681 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     4741 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     4801 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     4861 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     4921 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg
     4981 gtaccgtcga ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc
     5041 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5101 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5161 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5221 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5281 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5341 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5401 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5461 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     5521 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     5581 ttgattacta ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg
     5641 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     5701 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     5761 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     5821 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     5881 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     5941 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     6001 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     6061 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     6121 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     6181 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     6241 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     6301 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     6361 acttctccaa ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt
     6421 ctaatcctca tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc
     6481 cctaatgatc tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac
     6541 tactgcccct tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct
     6601 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     6661 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     6721 cccttttctt ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc
     6781 agcctgatct cttacctgtc ctataatttt ctttaattct ttattcatag attctactac
     6841 tccttgactt tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc
     6901 ttttttctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     6961 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     7021 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     7081 tgatgtttct aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac
     7141 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     7201 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     7261 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     7321 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     7381 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     7441 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     7501 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     7561 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     7621 ggctcgccac tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat
     7681 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     7741 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     7801 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     7861 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     7921 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     7981 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
     8041 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8101 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8161 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8221 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8281 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8341 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8401 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8461 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     8521 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     8581 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     8641 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     8701 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     8761 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     8821 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     8881 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     8941 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9001 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9061 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9121 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9181 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9241 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9301 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9361 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9421 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgg
//
