LOCUS       sequence-407467-        8265 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SNTB2_ZCRB1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8265)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SNTB2_ZCRB1
FEATURES             Location/Qualifiers
     source          1..8265
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3026..3736,3737..3739,3740..3742))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3068..3089)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3417..3436
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3676..3697
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      3739..3748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3824..4027)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3853..3873)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4028..4331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(4588..4632)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4817..5050
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5410..5527
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6019..6144
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6191..6448
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        6450..6829
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6860..6964
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6965..7033,7034..7825)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7183..7202)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(7996..8265,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagt
     1801 cggataattc ctcttcatca ctaaagtatg tagatttctt gatacgcgga cggcgactgt
     1861 catcagaggt gctaggtact cccgaagaag gcttccattt tttttgctct tcttcgattt
     1921 tagcctgctg aaacgcgatt gcctggctta agctgtctaa tgccgggtct tctccctgct
     1981 ctgccaacca cgcaatgtgc ttaacttctt tcgacccccc agcggtactc gtggctccca
     2041 acatcgcatt taattccgcc aggacttgtg gaagcaacgc cataatgtta gtatggattg
     2101 ccacgaacca ggagtgggca gttgccgtgt ctttgcagcg taaaataaga gtgttgcgcg
     2161 aatctgggga gtgcagctca atcaaacggt tttctaagtc tggcatgctc aggttacgag
     2221 cggcgaagca cattttcagt gggataatct tgcgatcctt agtcgaattt tggtgtttag
     2281 gtgagcccga gtcttccgag ccagagaacg atgggctttg aggagctgct ccttcccaag
     2341 gcaggtcaga taccagggat ggctttttga tatatggagt cacttcgcga atgaacttca
     2401 cttccaacaa tacctctttg ccggcgcgct tcaaagcctg tacggcttgg tcgtgagtgg
     2461 cctgacgtaa gtcggtacca ttgacagaca gaatcgcatc acctaaacgc aaggcgcgac
     2521 tctgatctgc agctaaaccg ggaaagatct tactgatcag aattggcatg cggttttcgc
     2581 ggccaccttt gatactaatg cccaggccgc ctgcttcctg cttgactaca cggacacggc
     2641 gaactggggg gctcgccccc gcctcaccgg cggggccacg gggcggagcg ggcggtgacg
     2701 gtggacccaa tccgcgagag ggcgaaccgg gtaagctatc cccagcgccg ccaccattgg
     2761 gcagcccatt gaaagctgca gccgcgggac caagagcggg ctccagttct gccgcggctg
     2821 cgtcacccgt aaggctcaaa ctttcgccgg ataactccgc cactacacgt acccaacgct
     2881 cacgcaacaa taactcaaca agtccggcct tggttgcgcg tgtccacact gccatagcag
     2941 ggcctgcccc ggctgctgcc gttgctgcgg caacacgcat tccccgggaa gcggccgcac
     3001 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     3061 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     3121 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     3181 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     3241 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     3301 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     3361 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     3421 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     3481 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     3541 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     3601 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     3661 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     3721 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     3781 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     3841 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     3901 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     3961 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     4021 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4081 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     4141 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     4201 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     4261 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     4321 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     4381 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     4441 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     4501 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     4561 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     4621 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     4681 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     4741 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     4801 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     4861 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     4921 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     4981 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     5041 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     5101 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     5161 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
     5221 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
     5281 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
     5341 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
     5401 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     5461 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     5521 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
     5581 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
     5641 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
     5701 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     5761 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     5821 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     5881 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     5941 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     6001 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     6061 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     6121 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     6181 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     6241 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     6301 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     6361 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     6421 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
     6481 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     6541 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     6601 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     6661 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     6721 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     6781 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     6841 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6901 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6961 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7021 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7081 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7141 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7201 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7261 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7321 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7381 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7441 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7501 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7561 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7621 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7681 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7741 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7801 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7861 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7921 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7981 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8041 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8101 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8161 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8221 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
