LOCUS       sequence-407470-        9825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, RCSD1_ABL2 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9825)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_RCSD1_ABL2
FEATURES             Location/Qualifiers
     source          1..9825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4065..4076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(join(4586..5296,5297..5299,5300..5302))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4628..4649)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4977..4996
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5236..5257
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5299..5308
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5384..5587)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5413..5433)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5588..5891
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6148..6192)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6377..6610
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6970..7087
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7579..7704
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7751..8008
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8010..8389
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8420..8524
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8525..8593,8594..9385)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8743..8762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9556..9825,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacctctgca
     1801 ccacatcact gatttcctgt acacatgaca ataagttatt aaggacaggg tttgtcccgg
     1861 gcacaccagc agctgctgaa gaaacctgta gctcctgcag gctgagttcc agtttgctca
     1921 cagcctctcg gaaggcaaat ttgttgcgag tttgagggat gcagtccaca tagcctgagc
     1981 agtagtcaag cagctggtgt ccagtgtcta ccagctggct gttgggcaca ggttccgtga
     2041 gtgcactgga cagtaggtca gcacattcca gcagggcctc tttgctgatt ttgtctgctg
     2101 agattttctc agcggcctgt ttggtttttc tcagagccac tttagtacct gctgtgccat
     2161 tggccatttt ggctggcgag atggaagatg tgggcagagg cacttgaggt ggaggcatca
     2221 ctggcctccc agctttccca ctgatgggca ctgcgcccag agctgccttc tttcctcctt
     2281 cctgtgtttc tgatgtggac tgtcctgcag ttagggcagt tggctcttct gtagggtctg
     2341 agcagatgga cggatgctgc agtagtctca tcactggtgg tgggggtggg gcacactttg
     2401 gttttacccg tcggggtcgg tccttgtctc cagaggatgt gacctgatgc tcagataaga
     2461 gcttgaattt attcccctga gagtctgtgc caatgagctg cacgtcagct ggagtgtgtt
     2521 tcagagtggg tgagataagg actggcactt tgtggttgtg agtggttggg aggacggggg
     2581 cagccttggc tggagaaggc cagcccggct gctctccatc ctctggaact ccagccatcc
     2641 caagtcgtgc cccaccattc ttctctttac ccttgggggc agctgccact ccagccactc
     2701 ccacccctgg agggtccttc tctgtaatgg ctagatcccc agagggtgtt ctgagaggaa
     2761 gagctgtggc tcctctgggc aataacttgg cttttggtct ctccctgctt ggagcagcac
     2821 tttcctctga tttttttgga agcatgtcat tggccctgtc cacattctct tctggctgag
     2881 aagaggtgga cactgtcctt tccagctgga gtttggacct ctggcagttc ctgggaaggg
     2941 tcattgccat cctatcctgc tctggaagcc ccgtgagttc gtatttctta tggggctgat
     3001 tctccatttc tcggaaggag ctgctgcgtt tggggggtgt aggagcattt ctcttcttca
     3061 tgaaggagct gaagaagccc cccttcctat ccctggtgaa gcatgtctct ttggcatctt
     3121 ccaagaggct gctgggtgac ttgtctcttt gctttcgagg cagtgctggg gatccactag
     3181 aggcctgtgc acctctgatg aaccctggtg ctaaactgga agcagaattt tctgtggcat
     3241 cttgtgcccc ttcaatgttc tccttgttct ccacctgttt cttcagtgtc cgagtcttgg
     3301 aaggaagtat aggtagccgg ggcaggtatg gaacaacaga tgacgaggag gcggctctcc
     3361 caagctcctc agctacctct tcagaaatgc tggagtcatg gaacatggtt tcaaaagctt
     3421 ggtgtgtttc agcaaaagag ggcctatcgg cagggctcca cttccagcat gctctcataa
     3481 gttcataaac cttagggggg catccctcag gctgttccat tcgatatcct ttttctagta
     3541 ggtcatagac ctgagacagg tcaatacctg gatatggtga cattccatag gtagcaattt
     3601 cccacaacaa taccccaaaa gcccagacgt cagatttaat tgagaaggta ttgtaggcaa
     3661 gactctctgg tgctgtccac ttaataggaa atttggctcc agcatgagca gtataagtgt
     3721 ctccagtcat caatctactt aagccaaagt cagccacttt taccacatgg ttttctccca
     3781 ctaggcagtt acgagctgca agatctctat ggatgaaatt cttcttctct aagtactcca
     3841 ttgcagaaga aatctgagtg gccatgtaga gcagcacaac tgcagtcacc tcttctcggt
     3901 tgcattctcg gaggtaatcc agcaaattcc cgtatggcat gtattcagtc acaatgtaaa
     3961 atggtggctc caaagtacac acacctaaaa gttgtaccag attaggatgc ttgatttcct
     4021 tcattactgc agcttctttc aggaattctt ctacctccat ggtatcttcc ttcaatgttt
     4081 tcacagcaac tgtaaggctg tatttcttcc agacgccaac gtaaacctct ccatactgac
     4141 cgcccccaag tttgtgcttc atggtaatat ctgttcgctc catttcccat ttgtcgtgga
     4201 tgggggacac accatagact gtaggcttat tacacttggg tgctgggtag tgtaatgttg
     4261 tcaccagccc atcagccact gtggagtgat ggtgtacaag ctctgccaag gtgctgaagc
     4321 ggctctcagc agtcacatac acctcctcac cattctggcc caggtctacc ttggggggga
     4381 acagggggag ggaacagggc ggtttccttc gggttggttt actggctggt gtctccttgg
     4441 ctgcagccgc ctgctcccta aaccgcccgg ccagctgggc caccgagggg gacgccgagt
     4501 tgtccacatt ggcattggtc tctgccggtc tttcctccat tccccgggaa gcggccgcac
     4561 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     4621 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     4681 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     4741 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     4801 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     4861 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     4921 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     4981 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     5041 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     5101 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     5161 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     5221 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     5281 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     5341 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     5401 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     5461 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     5521 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     5581 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     5641 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     5701 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     5761 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     5821 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     5881 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     5941 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     6001 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     6061 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     6121 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     6181 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     6241 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     6301 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     6361 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     6421 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     6481 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     6541 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     6601 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     6661 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     6721 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
     6781 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
     6841 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
     6901 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
     6961 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     7021 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     7081 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
     7141 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
     7201 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
     7261 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     7321 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     7381 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     7441 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     7501 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     7561 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     7621 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     7681 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     7741 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     7801 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     7861 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     7921 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     7981 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
     8041 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     8101 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     8161 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     8221 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     8281 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     8341 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     8401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8521 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8581 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8641 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8701 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8761 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8821 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8881 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8941 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9001 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9061 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9121 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9181 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9241 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9301 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9361 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9421 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9481 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9661 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9721 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
