LOCUS       sequence-407472-        9762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, PAX5_FOXP1 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9762)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_PAX5_FOXP1
FEATURES             Location/Qualifiers
     source          1..9762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4523..5233,5234..5236,5237..5239))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4565..4586)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4914..4933
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5173..5194
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5236..5245
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5321..5524)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5350..5370)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5525..5828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6085..6129)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6314..6547
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6907..7024
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7516..7641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7688..7945
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7947..8326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8357..8461
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8462..8530,8531..9322)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8680..8699)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9493..9762,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 ccatgtcttc gttaacgggt tcgtcttcat aatcgcgatc atgatcgaaa tccggggaat
     1861 ggttcgctgt agtcaccagc gacagggggc cctcggcttc ctccggatct aaaggctcct
     1921 cctttacgtg aactgggtga accgcctgca ttgggctacg acctggggag gagtcagatt
     1981 cattggaatt tgtgtgctcc attgctccgt ttaattcttc gcggattgcg ctagctaaat
     2041 taccaagggt tgggtttccc atagatgcag tagtatataa cggaattgaa ttttcggcca
     2101 tacttgcctg cagcgcagca ttcaacgggg tacagtaagc atgcgaggac tgcatgtttt
     2161 tgattaacga tggattgtga gccaccagtt cgtcgaaatt ttcatctgga caatacttgc
     2221 ggggacggcc acgctgaatg tgacgaccgc gtttaaactc ctcatcgtca acggtccaaa
     2281 agctaccaaa ttcatcttct acacggataa agcacttgtg aaggcttaaa ttagtacgaa
     2341 ttgcgccctt ccacgtagca gcattacgac gaaaatacgc aaacatacgc gtaaaccagt
     2401 tataaatctc atttaatgtc agttgctttt caggtgattc aaggatcgct tgacgaatta
     2461 agctggcata ggtaaatggt ggacgaacct ctgcgttctt gtaaaattcc tggttttgag
     2521 caatgtcagc ggaagaaatg ggtacgttat acttgtccga ataacggcga cggatagggc
     2581 cgacagtatg catcgaggtt gttgtgatta ctgacggccc ttgtgttacg ggagtaagtg
     2641 gtgccgtagg agtggttggg gtgtggggca agctctgtgg actggcttca gaagcactct
     2701 tgcttaaggt gacggaactg actaagttca acggttgagg agctgctttg ggttcggtag
     2761 actttacatg aaggtgcgtc atcatcgcct gcaagcgttc tttgtctttg gctaattgta
     2821 actcaagttg ctggacgact tgcatttgaa cgcggcattg cgcagtagaa cggtcgtcaa
     2881 gagcatgttc ggagtttaaa tgtttcagaa aactctggaa atcctcgcag accgcttcgc
     2941 agccaggcca tttgcaaacc ccgtgaccgt aaagcgggtg tgaatgagga tgttcttcgt
     3001 ggctcagact ttcacgtttg ggagtgtgca ctgataattg accgttggtt gaggcatgcg
     3061 gattcataat caaagacgtc ttactgggtg ctgacgaact cacacacgta gttgtcaaat
     3121 ccaatgagct gtggttgtta ccggttgttt cttctgcagt gtgcgcgctc gtaacttctt
     3181 tccataactg ttgcaactct gtcgggatca taccttgagc caggggttgt aatggcagag
     3241 caggttggcc gggttggatg gttaataatc cttgacgctg taatgataac agatgctgct
     3301 gctgaagttg ttgcatttgc aaaagctgct gttgaaaggc taattgctgt gtcgcaactt
     3361 gctgctgttc tttcggctgt ttaccagcgt gctgctgttg taataactgc aattgaagtt
     3421 gctcttgttg ttttttgtaa aactcttgca gctgctgctg ttgcaacatc agggcttgtt
     3481 gttgctgtaa aagtacttga agttgctgtg gtgacagcac ttgctgctgc aaaatttgct
     3541 gcatttgttg aggtgtgatt acttggggcg tcatcatcgc cacagacacg ggaacttgca
     3601 gagcaggctg cttgtcgttg cgtttaggac tcttaagtcc ggatacctgc tgctgctgct
     3661 gttgttgcag taacagttgg cgcgccactt gcaatgcctg ctcgggttta atgggttcgg
     3721 tcgtagtaaa gatgtcggaa taatgctgac gctcaaacac acggtcaagg acttccaatt
     3781 gctgctgtgt gaataagtcc ccacgcatct gtttgcgcaa aaaatcgcga cctggaagag
     3841 agtgtccatt cggaaccggc gactcctgaa taccttcatc gcgtttgcgt ttattcgtgt
     3901 ctgcgctcgg agatgtaatt cccaggatac cgctgatgga gtagctagac ccagccgagt
     3961 ccgtacttac actgctaacc tgggtaacag aaccagtact tacaatgctg tgtgaggagg
     4021 cgggaacagg ctgattaggt ggctgctgta ctttcgtgcg aataatacgg ttgatcgagg
     4081 agactgatgg cacggtatcg ttgtcacaaa cacgttcggc caaaagacga tcacgaattt
     4141 cccaagcgaa cattgttgga ttctggcgct tatattcggc aattttctcc actaccttag
     4201 gagtagctac cttcggcttg gagccgccga ttacaccggg cttaatactg cctgtctcat
     4261 agtagcgtcc caaaatcttg ctcacacacc cgtgtgaaac gcgaagttgg cgagagatgt
     4321 cacacgggcg tactccttgg tgggccaact caacgatgcg ctgacggaca acatctggca
     4381 gtggacgacc gtttacgaaa accccgccaa gctgattgac accaccatgc cctgtgcggc
     4441 tagtacgtgg tgtcggatag ttcttttcca gatccattcc ccgggaagcg gccgcacttc
     4501 ccccggagcc tccgcttccg cccttgtaca gctcgtccat gccgagagtg atcccggcgg
     4561 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcagct
     4621 tggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     4681 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     4741 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     4801 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     4861 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     4921 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     4981 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     5041 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     5101 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     5161 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     5221 cctcgccctt gctcaccatg gtggcgaccg gtggatcccg ggcccgcggt accgtcgact
     5281 gcagaattcg gttcactaaa cgagcccggt tcactaaacg agctctgctt atatagacct
     5341 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     5401 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     5461 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     5521 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     5581 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     5641 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     5701 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     5761 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     5821 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     5881 aataactagc acgcgttcga agcttgagct cggtcccctc ggggttggga ggtgggtctg
     5941 aaacgataat ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac
     6001 tattcttaaa cctaccaagc ctcctactat cattatgaat aattttatat accacagcca
     6061 atttgttatg ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg
     6121 ttcattcttt tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta
     6181 attgttaatt tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga
     6241 tttattactc caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca
     6301 accccaaatc cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc
     6361 ctggagctgc ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc
     6421 cctcagcaaa ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt
     6481 cagcgtcatt gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa
     6541 agctcctatt cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg
     6601 tgctactcct aatggttcaa tttttactac tttatattta tataattcac ttctccaatt
     6661 gtccctcata tctcctcctt atggtgtttt actaaacttt tccatgttct aatcctcatc
     6721 ctgtctactt gccacacaat catcacctgc catctgtttt ccataatccc taatgatctt
     6781 tgcttttctt cttggcacta cttttatgtc actattatct tgtattacta ctgccccttc
     6841 acctttccag aggagctttg ctggtccttt ccaaagtgga tttctgctgt ccctgtaata
     6901 aacccgaaaa ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt
     6961 tgctattatg tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt
     7021 aaaattgtgg atgaatactg ccatttgtac tgctgtctta agatgttcag cctgatctct
     7081 tacctgtcct ataattttct ttaattcttt attcatagat tctactactc cttgactttg
     7141 gggattgtag ggaatttcca ggtctgaaga tcctgctgct tgctgtgctt ttttcttact
     7201 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
     7261 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
     7321 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
     7381 caggccagta ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
     7441 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgcg atctaattct
     7501 cccccgctta atactgacgc tctcgcaccc atctctctcc ttctagcctc cgctagtcaa
     7561 aatttttggc gtactcacca gtcgccgccc ctcgcctctt gccgtgcgcg cttcagcaag
     7621 ccgagtcctg cgtcgagaga gctcctctgg tttccctttc gctttcaagt ccctgttcgg
     7681 gcgccactgc tagagatttt ccacactgac taaaagggtc tgagggatct ctagttacca
     7741 gagtcacaca acagacgggc acacactact tgaagcactc aaggcaagct ttattgaggc
     7801 ttaagcagtg ggttccctag ttagccagag agctcccagg ctcagatctg gtctaaccag
     7861 agagacccag tacaggcaaa aagcagctgc ttatatgcag gatctgaggg ctcgccactc
     7921 cccagtcccg cccaggccac gcctcccatg gtaatagcga tgactaatac gtagatgtac
     7981 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     8041 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     8101 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     8161 actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca
     8221 ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat
     8281 gaccccgtaa ttgattacta ttaataacta gtcaataatc aatgtcgacc caggtggcac
     8341 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     8401 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     8461 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     8521 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     8581 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     8641 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     8701 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     8761 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     8821 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     8881 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     8941 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     9001 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     9061 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     9121 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     9181 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     9241 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     9301 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     9361 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     9421 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     9481 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     9541 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     9601 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     9661 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     9721 accagtggct gctgccagtg gcgataagtc gtgtcttacc gg
//
