LOCUS       sequence-407474-        9300 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, TCF3_FLI1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_TCF3_FLI1
FEATURES             Location/Qualifiers
     source          1..9300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4061..4771,4772..4774,4775..4777))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4103..4124)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4452..4471
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4711..4732
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4774..4783
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4859..5062)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4888..4908)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5063..5366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5623..5667)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5852..6085
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6445..6562
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7054..7179
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7226..7483
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7485..7864
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7895..7999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8000..8068,8069..8860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8218..8237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9031..9300,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagt
     1801 agtatgaacc aaggtgggag ggtacatggg tatttggatg gcgggggacg ttcgggttag
     1861 gataaattcc tccagtcggc gaagtccagt attgactcgc ggcaccaaag aaggacgagg
     1921 aggtaactgg catggaacta gggtgaggcg gcacgaagtt aaccttttgt tggtgagcat
     1981 ggtaggacgg catgtatgaa atatcgctag ggtatttata catagatgac tcggttggat
     2041 gaggctgtaa tgcttgggca ataccgtgaa agtcaaattt ataggcgtaa cgcttcccgt
     2101 gcacctttgt catgatattt ttatcgtagt aatagcgtaa cgcacgcgac aacttgtcgt
     2161 aattcatgtt gggttttgat ttgcgctcac cccagcggcg agcgacctca tccggatcgg
     2221 tcattttgaa ctcaccatta gttccctccc aggtgatgca gcttgcattg gccgagtcgc
     2281 tcagtaattc taataagaat tgccacaact gaatttgtcc agaccctgga ttggccaggc
     2341 gacttgaagt gggtcctaaa atctggtacg gatcaggttg cggacgttgc tccgtgttct
     2401 tggaaattgt ctgagcccca cccagcggag gagatttatt aagaccgctg ttcatattgt
     2461 taccccacgc tccacggcgg acggagtcat agcttgggtc ctcttttact gacagacgtg
     2521 aggattggtc ggtgtgagaa gtcgtgttgt acgccaacag ggaaccgcta tatgagtctg
     2581 gagggcgaga caggtctggc agcgtaccag gttgtgatgg aagggctgcg tggttgtgca
     2641 taagagaggt actgcctgcc agcccatctt ccggatgact gccaccaacc agtccggcgt
     2701 gacgaccacc cagtgacata ggacctgtaa aacctgaagc cagagctccg tgccctggaa
     2761 gtaaggtgtg catgtcacca gctgtgccca ccgcatgact acgtaagaca tggatggcct
     2821 cgtctaagtg gtcttcgatc tttgactgca atccatgaag gccaccatca taagacgggg
     2881 acagagcccc tggggcaccc gcacgtggcc actggctcgt accagccaaa ccttgaggac
     2941 tacctactgg tgtggaagga ctagacgaga aattattgga agaatggtcc ggtgaataaa
     3001 ttgacgctaa tgccttacca agggcgtctc ctgaactacc agcagtcgta ccacgcgatc
     3061 caagcaaaga gtctgctcca ctcacagggg gcgtgtgcga agaaacgcca ccataggtcg
     3121 cgccaggggc gctagaaaat gatgaagcgc taggtaaacc tccgtttact tctgccccat
     3181 gcaattggta gcccatgcgc tcgtgctggt gtaacccacc gaacgtggaa ctggaacctg
     3241 acgagcctac tgggccggag ccgggtggca atggcaaggg cgaagaacca cccccaagca
     3301 ttggtccaaa ccctgcctga ccaggaggag accaaagttc tgcagaagga tgcagcgatc
     3361 catccgcaac gtaaaacggc gcgggatatg tagacgaggg agttttagcc gaagggtacg
     3421 cagttgcatc gcgaccgtag tcttcgccag acgacggcgg gtatacacta ctgggcagtc
     3481 ctggcggcac tttacgtacc tttttgggtt gggtgtctaa tgaaccatca gccgcgcggc
     3541 gacgagagga gcccgagtag cttgggtagt attgtgatgt gcccttcatt ccggacggag
     3601 ataatggccc cggagagttc aaggctaact ctcctgacag gaatcccgcc tgagttaacc
     3661 ctcccacccc tgcatcacgg ccaaaagaag cgtaggcacc acgttcaccg gatttccctc
     3721 ccaagcctgg tcccaagaac gtggagcttg acagactcga atgcgactcg gtgaaatgag
     3781 tgccttccga aaacgtgcgt gaagggtcga aactgcttga actctggtcg ccgctacccc
     3841 aactgcccga ggacgggcgg tcctcaagac ccgatccgcc aaactgagcc ccagccaagg
     3901 acgcggggcg acctttacca tttgtaaccg gcagaggaaa catcatcgaa aaatccagca
     3961 gatctgacag ctctttgtcc gtgccgacgg gcgccatgcg ttgaggctgg ttcattcccc
     4021 gggaagcggc cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc
     4081 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     4141 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     4201 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     4261 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     4321 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     4381 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     4441 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     4501 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     4561 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     4621 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     4681 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     4741 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
     4801 cccgcggtac cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag
     4861 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     4921 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     4981 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     5041 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     5101 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     5161 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     5221 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     5281 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     5341 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     5401 ccgtaattga ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg
     5461 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     5521 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     5581 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     5641 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     5701 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     5761 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     5821 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     5881 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     5941 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     6001 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     6061 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     6121 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     6181 taattcactt ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc
     6241 catgttctaa tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc
     6301 ataatcccta atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg
     6361 tattactact gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt
     6421 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     6481 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     6541 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag
     6601 atgttcagcc tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc
     6661 tactactcct tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg
     6721 ctgtgctttt ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     6781 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     6841 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     6901 gccttctgat gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc
     6961 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7021 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     7081 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     7141 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     7201 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     7261 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     7321 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     7381 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     7441 tctgagggct cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg
     7501 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     7561 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     7621 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     7681 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     7741 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     7801 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     7861 tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     7921 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     7981 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     8041 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     8101 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     8161 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     8221 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     8281 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     8341 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     8401 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     8461 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     8521 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     8581 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     8641 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     8701 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     8761 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     8821 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     8881 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     8941 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9001 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     9061 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     9121 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     9181 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     9241 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
//
