LOCUS       sequence-407475-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SUCO_CRTC2 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SUCO_CRTC2
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4391..5101,5102..5104,5105..5107))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4433..4454)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4782..4801
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5041..5062
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5104..5113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5189..5392)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5218..5238)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5393..5696
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5953..5997)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6182..6415
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6775..6892
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7384..7509
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7556..7813
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7815..8194
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8225..8329
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8330..8398,8399..9190)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8548..8567)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9361..9630,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcatt
     1801 gtaagcggtc actacggaaa gactcctcaa ctgccggatc aggcagtaac gcacaagggt
     1861 ccgaaagcat gttaagtcct tctaaaccta agggttccat acgtaattca tcctctaagc
     1921 ccagtcctaa ttctaaaccc gccgcactaa cctcgaaacc agggacccca gcaagtgccg
     1981 cagcaatttc ttttgagaaa ccaggactac tgtcccccgt caaaatgata ttagggcctg
     2041 acccatgacg agagcaatgg gttaagtttt ggtggttgaa agtgtgaggg tcctgaggtc
     2101 cccccatcgg gccttcgccc cccaagaacc ctgggccctc cgagaaacca gggggatcaa
     2161 gaactaatga tgcagagggg ctttccatac tgaactgttc cagattccct aaattgaaat
     2221 cgctcattgg acggtgatag gattgttgcc cgtgacctga gggtcctggg gggtagggtg
     2281 ttccgtaatg cgattggcga ccgggcggtt gaccacctga tgattgcacg gagcacgatt
     2341 gagatggcag accgggctgc tgaagggact ttggtgtatg cggttgtgtt ggcaatacaa
     2401 gggatggtga ggaatagggg taggggggca agcgctgatc cgtggacaac ttggatgtgt
     2461 caagaggaac tccttgcgta atgctcgaca aagtaggact cattgttggc gagaactgct
     2521 tgggtaactg ctgctgtgag cgacgcgcat ctgcagggcc tgccaaaagg ctaagtggcg
     2581 acaatggaac gcgacggtgg tgtggggacg cccccggagt gctcgcagga tatgaagggg
     2641 ctccaagtac tggagaactc gttgatgaag aagacgaaga tgaagacaga gctggtgcgg
     2701 aaagggaagg atgccctaag gaagtggtgg gaagtacatg gcgtgctaag gacgatgcag
     2761 gaagagatgg atggctgtgt gagccttgta actgcggctg aggggatgat aaagaagcct
     2821 gaaggttagg gttgctaagg ctcgactgta aactagggtg ggacaggggt gagtgaagtc
     2881 cgggagcgtc atagccgggt cccagtccca tcccgcgtga aatacccagg tgggtcattg
     2941 tgtgggtaag gttagacgtg gaattaccac ctgacagcga agggtaggcg gtctcttccg
     3001 ggtccaaagg cgttggtaat ggtggtggga aatgaagatt ggtcaggtcc ggaagcgagc
     3061 cgcccgtatt cattgcaggg ggcagcaccg gtacattagc cggctggtcc ggcgacggaa
     3121 agatgttaat acctggcacc tcgcatgagc gaggacgaga ggacgaggac gataacttct
     3181 tggcatccca gggcttaaga aggtgtttgt cgtccaacaa gttttcttca atcgcaggca
     3241 ctttcgggtc catttccccg tccaagatac cgccacgacg agacggaaga atagaaggcg
     3301 gggttggacc tgggtacgtg tcttgtgggg aagggttcat gactgaagta tgcagtgccg
     3361 agtcgctcga agtacgattc agggcagagg gtaaacggaa caattgaccc ttctcagctg
     3421 gaaaatttcc ccaagccatc gtgcggcgcc aactagattc gggtggcggg ctcaggtaag
     3481 ccggagaata aggggacgaa tcgatgtgac gagtgtagcg gcgcagtggg ctaaccatgc
     3541 ggcgaggatc gcgctgaacg cgctcaacta acccatgatg gcgtgtgctg cgagatgagt
     3601 caaggggtga atgaagtggg ctctgaaatt ctgccagtcc gctcccaatt tggttcacgt
     3661 tcggcagtga gcccccgtaa tggctactac gagtgtaggc taagcgcagt ttttgggcct
     3721 gcaattcatt cttcaaatta tcggacgcgt tcaaagctga cttcggatca cccccgccct
     3781 gctcagaggc cgatacttta gactcgaact ccgacttcgt gattttacct ttaccgtggg
     3841 agcttgatac attctcaatg tgctggccaa ccagagagtc gggtggagaa acaaagctag
     3901 gttgttcaat cggggcactg gcatccaggg cctcgccgac gtcgcagtcc gtctctgatt
     3961 gttcagtctc cgagggcttc gcgattggaa ttgtcccact tttctcaatt tcatcaagtt
     4021 tcgaaatggg ggtaatttct gaagtacttg aggagctaat attctcaaca gtttctgaat
     4081 ctacgaccgc attactgctt tcctcatgaa ggtcaactgt tggtaaggtc tcaacaacag
     4141 gtgggcttaa cttttttgac tctgtgtttt ttttttggaa ttgtacgtct tcattctcaa
     4201 gggcgcaatt gtcatcctga ctataatagc tactagccga cgcggaagat gattccttgc
     4261 aacagacacg ccaagacggc aaccagacaa gcgaacacag gaataaacaa gatactaaag
     4321 ccaaagcacg gcgatgcttt ttcattcccc gggaagcggc cgcacttccc ccggagcctc
     4381 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     4441 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     4501 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     4561 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4621 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4681 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4741 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4801 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4861 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4921 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4981 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     5041 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     5101 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     5161 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     5221 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     5281 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     5341 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     5401 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     5461 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     5521 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     5581 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     5641 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     5701 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
     5761 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     5821 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     5881 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     5941 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     6001 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     6061 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     6121 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     6181 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     6241 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     6301 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     6361 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     6421 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     6481 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     6541 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     6601 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
     6661 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
     6721 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
     6781 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     6841 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     6901 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
     6961 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
     7021 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
     7081 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     7141 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     7201 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
     7261 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     7321 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     7381 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     7441 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     7501 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     7561 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     7621 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7681 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     7741 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     7801 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     7861 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     7921 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     7981 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     8041 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     8101 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     8161 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     8221 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8281 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8341 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8401 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8461 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8521 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8581 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8641 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8701 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8761 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8821 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8881 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8941 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     9001 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     9061 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     9121 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     9181 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9241 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9301 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9361 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9421 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9481 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9541 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9601 tgccagtggc gataagtcgt gtcttaccgg
//
