LOCUS       sequence-407478-        9819 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SSBP2_JAK2 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9819)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SSBP2_JAK2
FEATURES             Location/Qualifiers
     source          1..9819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4580..5290,5291..5293,5294..5296))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4622..4643)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4971..4990
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5230..5251
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5293..5302
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5378..5581)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5407..5427)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5582..5885
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6142..6186)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6371..6604
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6964..7081
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7573..7698
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7745..8002
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8004..8383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8414..8518
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8519..8587,8588..9379)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8737..8756)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9550..9819,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaac
     1801 ccgccatgtt gtcgcggatt tgatccacac gaagcgcaag atcacggaag ctcggacgtt
     1861 gattaacgtt attgttccaa cactcagtca tgatcatata gatttcatca gggcacccgt
     1921 cgggacgtgg caggcggccg ttgtttttca gaagctcaat taagtgaaag acaatcattt
     1981 ggccttgttt gtcgttgcca atcatgcgca taaattcggc cgggggactc ttggattttt
     2041 caatgtaagt gaacagctcg tacaaaacga cgccaaagct ccagacgtca ctggccacag
     2101 agaatttgct ttcggtaaga ctctccgggg cataccaaaa aataggagac tctcccggtt
     2161 cctttacttt ataatactcc ttatcttgcg gcagtacctt agttaatccg aaatctccaa
     2221 ttttaacacg attttcgttc tctactaaaa tattacgcgt agctaaatcg cggtggatgt
     2281 agcgcttggt ccctaaatat tccatgccct tgcagatctg actggtatat tgtaacagct
     2341 taatgtggtc aatacgttcc ttgtgttttt gcaggtaatc acgcaatgac ccgtaaggca
     2401 gatattccat aattaacttc aggttacgac gtcccgccga gtagcagacg cctttatact
     2461 tcacaatatt atcgtgctgt aaactcttca agatctcgat ttcacgctcg aaatcgcgca
     2521 gatgttcctc agtactatgc tgcaattttt ttacagcaac tacttctccg gtattgtctt
     2581 gaagcggatc atagcggcac atttcaacgg agccaaaatt tccctttcca agttgttgca
     2641 aaaatttcaa atggcgttcc tcaaattgtg ttgggtcgcg gtcctcgaat gctcctgaaa
     2701 atcccagtgc gccgatgcgc atgttcggca gcatgtcgtt ctcagtcaaa agctcatagt
     2761 caggggtgaa aagggaattt aagtcacgga tgatcgcgcg aaatgatgga cggaaatcag
     2821 gctcataatc catacaatta ttgataagat tggccagctc tgcccatttt ggtgctggca
     2881 actggtggcg atcttcataa aactgcaatt tgcgctggct atctaatgcc gataaaggtt
     2941 tatctccccc cgaacagatt tcccacaggg ttgtgccgaa gctccattta tccgttgcca
     3001 ggttaaggtt cttggggttt tcaatgcatt caggtgggac ccatgggatg cgctcttgaa
     3061 gaatgtcctt cggcagcacg gtaattgaga tgcctgggtc gctaagttta atgaagggtg
     3121 gattccccgt cttgcgatct tcctcgcgga tcagcagaat atttttcgca catacattcc
     3181 cgtgaatcag agtgttctcc tcaagaaagt gcatggccca agccaattgc ttagccacct
     3241 caagtttcca caagatgtta atacaattct tgtttttttt caaataagta tcaaggctac
     3301 cgaatttgac gaactcttga accaaaatgt tttcatcccc acaaacacag actccgtagt
     3361 ttaagaccag gtgcttatgg gacagtttgg acatcatgga cgctgcttcg aagaacgatt
     3421 ctgagtagtt acggtgtgct ttatccaata cttttaacaa tacctcagtt tcatgtaatt
     3481 gcccgtaatc ccccacctca cggcgcaccc ccttaaagat ttttgtgaac gtaccctgcc
     3541 caagggactc attcttgcta atactatcca tatctccgga gcccaatgat ccattcatgt
     3601 ggtggctttc cattccgcct aagccaccca taggaccgtc tgaccctggg cccattggga
     3661 agttcgggcg gttcggacca ggaggaacgg cattcataag ggtatacatg ttgtcgcccg
     3721 aatttgtaga atcagccggg ctaggcataa ttggcgtacc cggtggcccg ccaccaccgg
     3781 gtgggcccac atagttacct ggggatgcac tactgtatgg gatagagttc gcgttagtag
     3841 ggttgggcca tgggcgtccc ccaccggggc ccatgttcat tccaggcatg ccgggtcctc
     3901 ccaaggcgtt cagtggcggg cgcattgctc cgccgtaatt ctgtaaagac aaccaggggt
     3961 cagactgagg cccaagcggc accataccgc ggggcggagt catacgttgc atcggcccac
     4021 ccatatttgg atgcccctgc tgacgggttg ggtccatgcc tgatggcagc agtggctgtg
     4081 atccaggcac gccgccaaga gcttggtttg gaatgcgtaa cggcgggcga ggaccacccg
     4141 gataacgcgg actcatgaag ggttggaaga aaccgggcgg aacaggtcca accggcatac
     4201 catcccctgg cggaatattg cccagtactg gggaaggtgc ggctgcagcg ctataatcgt
     4261 gaaacgcctt tgcctcactg ctgtgttcgc atgtttcacg gcgttcaggt gccgcgcaat
     4321 acaaatccca gaaaacgcac caccaagaat gcaaaaaacc aggaggctca cccagcgtaa
     4381 tgttcttttc ccagcgaatt tccgacaaaa acgtttgtgc tgatttttga gcccctacat
     4441 gcaataaata ttcgtacacg tacagggcca gcttttcacg agcttgagag tcacttggca
     4501 ccgccgaact gttagattta ccctttccgt acattccccg ggaagcggcc gcacttcccc
     4561 cggagcctcc gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     4621 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     4681 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     4741 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     4801 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
     4861 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     4921 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     4981 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     5041 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
     5101 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
     5161 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
     5221 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
     5281 cgcccttgct caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca
     5341 gaattcggtt cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc
     5401 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     5461 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     5521 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     5581 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     5641 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     5701 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     5761 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     5821 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     5881 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     5941 aactagcacg cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa
     6001 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     6061 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     6121 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     6181 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     6241 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     6301 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     6361 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     6421 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     6481 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     6541 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     6601 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     6661 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     6721 cctcatatct cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg
     6781 tctacttgcc acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc
     6841 ttttcttctt ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc
     6901 tttccagagg agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac
     6961 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc
     7021 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa
     7081 attgtggatg aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac
     7141 ctgtcctata attttcttta attctttatt catagattct actactcctt gactttgggg
     7201 attgtaggga atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt
     7261 gttttgctct tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg
     7321 atgcacacaa tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt
     7381 ctgaagggat ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag
     7441 gccagtatta actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt
     7501 atattttttc tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc
     7561 ccgcttaata ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat
     7621 ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg
     7681 agtcctgcgt cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg
     7741 ccactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     7801 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     7861 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     7921 gacccagtac aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc
     7981 agtcccgccc aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc
     8041 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     8101 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     8161 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     8221 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     8281 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     8341 cccgtaattg attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt
     8401 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     8461 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8521 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     8581 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     8641 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     8701 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     8761 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     8821 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     8881 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     8941 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     9001 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     9061 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     9121 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     9181 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     9241 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     9301 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     9361 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     9421 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     9481 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     9541 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     9601 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     9661 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     9721 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     9781 agtggctgct gccagtggcg ataagtcgtg tcttaccgg
//
