LOCUS       sequence-407480-        9558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, TCF3_PBX1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9558)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_TCF3_PBX1
FEATURES             Location/Qualifiers
     source          1..9558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4319..5029,5030..5032,5033..5035))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4361..4382)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4710..4729
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4969..4990
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5032..5041
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5117..5320)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5146..5166)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5321..5624
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5881..5925)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6110..6343
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6703..6820
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7312..7437
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7484..7741
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7743..8122
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8153..8257
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8258..8326,8327..9118)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8476..8495)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9289..9558,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaat
     1801 tactggtgtc actatgaaca cttccgggcc cttcagtcgg actcgtgacg gatgacggcg
     1861 ttgtagcatc ctgccatccc ccatttgctg agatcccttg tggggaatac atttgactgg
     1921 cagctaaccc atcgctatat ccacccgtct gagaaatgac gtgacgtaac gtgtcaactt
     1981 gagactgtac attggcacct acttgagctc cctggtagga gtccccgttt aatgactgaa
     2041 cactcataaa caaatcaccg ctatttgaca tgttaaacga tgacgaggac ccggcggagt
     2101 ttggggtgga tggggaatta gcttgactgc catgggctga gacgttggtg gcagttacgg
     2161 ctgtcttagc agcataaatg tttgcctcct cttggaactt tccgatattc ttcttataac
     2221 ggatacgctt gtttccaaac caattgctta cctgcgatac tgtaattcca cacttcttcg
     2281 ccaactcctc tttggcctcc tcgctcggat agggattgga aagatgggaa tagaagtact
     2341 cgttcagaat ctcagtagct tgcttattaa agttacgacg tttacgacga gcgtccagaa
     2401 agcgagaacg taagatcatc acggcttcac aagtgctctg ttttaattgc atttgaatac
     2461 tgctgaactt acggtggata atgctaacca tacgctcgat ctcttttgga ctgataggac
     2521 gcgtacgact ttgctcacgc agaaggttca taacgtgagt ggtaaattcg ttgcaagcct
     2581 gctcgtactt ctctaattct gtatgataga tttgacggat ttgactcagt ttcgcacggt
     2641 aatcggagtg ttcaaccgag ttgtccgaac cggcgccacc ggaggccgct gcagcagctg
     2701 ccgctgctgc gctacccccg cccttctccg gtcctgcaac accctcggcc aacaacatgt
     2761 tgtccagacg catcaattgt gggtcagtag gttcttcctc ctgagcgccg cgaatggaca
     2821 ggacgctgta cgaatccggc gggcgactca gatcgggaag agtgcccggt tggctaggca
     2881 gcgctgcgtg gttatgcatc aatgacgtcg aacccgctaa tccgtcttcc gggtgggagc
     2941 cgccgactaa accagcgtga cgtcccccca gtgacatagg acctgtgaag ccagatgcca
     3001 gcgcgccgtg cccaggtaac aatgtatgca tatcgccagc tgttcctacc gcgtgcgagc
     3061 gaagtacgtg aatcgcctca tccaggtgat cctcaatctt agactgcaat ccgtgtaagc
     3121 ccccgtcgta tgaaggactc aaagcaccag gtgcccccgc gcggggccat tgtgacgtcc
     3181 cggctaaccc ttgaggactt ccaacgggtg ttgaggggga tgaactgaag ttgttactag
     3241 agtggtcagg tgagtaaatg ctcgccagag ccttgcctaa ggcatcacca gaactccccg
     3301 cggtggtccc acgggaacca agcagactat ccgcaccact taccggggga gtgtgcgacg
     3361 agacccctcc gtaagtggca ccgggggcgc tggaaaaaga cgatgcagag ggcagcccgc
     3421 cgttgacttc agctccatgt aattgatacc ccatacgctc gtgctggtga agtcccccga
     3481 aagtcgaaga actgccggaa ctgcctacag gcccgctacc cggaggaagt ggcaaaggac
     3541 tcgacccgcc acccaacatc ggaccaaatc ccgcttggcc aggcggcgac caaagctccg
     3601 cagatgggtg taacgagccg tccgccacgt aaaacggggc cggataagta cttgaaggag
     3661 ttttggcaga tgggtacgcg gtcgcatcgc gtccatagtc ctctcctgaa cttgggggat
     3721 agaccgaact cggtaatccg ggaggaactt tgcgcacctt tttgggctgg gtatcaaggc
     3781 tgccatcggc tgcacgacga cgcgacgaac cgctgtaaga aggataatat tgagaggttc
     3841 ctttcattcc acttggcgaa agagggccgg gggaatttaa tgctaactca ccgctaagaa
     3901 agcccgcctg tgtaaggcca cctactcccg catcacggcc aaaactggca taagccccac
     3961 gttcccccga cttaccgcca aggcccggcc ccaggaaagt tgagctggac aaggatgaat
     4021 gggattcggt aaagtgggtc ccttcagaaa aagtgcggga tgggtcaaag gaggaagatg
     4081 actgatcccc ggaaccccag ctcccagatg aggggcgatc ctccaggccg ctgcctccga
     4141 actgggctcc cgccaatgat gcggggcgcc ctttaccatt agtcaccggc agcggaaaca
     4201 tcatcgagaa atccaataag tctgataatt ctttatccgt tcccactggg gccatgcgtt
     4261 ggggctggtt cattccccgg gaagcggccg cacttccccc ggagcctccg cttccgccct
     4321 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4381 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     4441 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4501 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     4561 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     4621 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4681 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4741 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4801 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     4861 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     4921 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     4981 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     5041 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcggttc actaaacgag
     5101 cccggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     5161 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     5221 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     5281 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     5341 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5401 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5461 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5521 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5581 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5641 ggctatgaac taatgacccc gtaattgatt actattaata actagcacgc gttcgaagct
     5701 tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     5761 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     5821 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     5881 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     5941 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     6001 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     6061 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     6121 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     6181 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     6241 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     6301 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     6361 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     6421 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctccttatgg
     6481 tgttttacta aacttttcca tgttctaatc ctcatcctgt ctacttgcca cacaatcatc
     6541 acctgccatc tgttttccat aatccctaat gatctttgct tttcttcttg gcactacttt
     6601 tatgtcacta ttatcttgta ttactactgc cccttcacct ttccagagga gctttgctgg
     6661 tcctttccaa agtggatttc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     6721 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     6781 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     6841 ttgtactgct gtcttaagat gttcagcctg atctcttacc tgtcctataa ttttctttaa
     6901 ttctttattc atagattcta ctactccttg actttgggga ttgtagggaa tttccaggtc
     6961 tgaagatcct gctgcttgct gtgctttttt cttacttttg ttttgctctt cctctatctt
     7021 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     7081 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     7141 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccagtattaa ctgcgaatcg
     7201 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     7261 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     7321 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     7381 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     7441 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     7501 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     7561 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     7621 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     7681 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     7741 cccatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     7801 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     7861 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     7921 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     7981 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     8041 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     8101 taactagtca ataatcaatg tcgacccagg tggcactttt cggggaaatg tgcgcggaac
     8161 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     8941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9421 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9541 taagtcgtgt cttaccgg
//
