LOCUS       sequence-407484-       10890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, THBS1_FSIP1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10890)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_THBS1_FSIP1
FEATURES             Location/Qualifiers
     source          1..10890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(5651..6361,6362..6364,6365..6367))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5693..5714)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6042..6061
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6301..6322
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6364..6373
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6449..6652)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6478..6498)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6653..6956
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(7213..7257)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7442..7675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8035..8152
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8644..8769
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8816..9073
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        9075..9454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9485..9589
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9590..9658,9659..10450)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9808..9827)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(10621..10890,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacggttcct
     1801 tgcactcttc ggcagcgtcc ttggtttcct gctcgtctgc aatcgtgttc tccggggagc
     1861 tcagtttcag atgttgatct tcgctggata agctcacgct aataccgtat aacgggtcgt
     1921 ccaaaaagct aggtctctta aggcgaccga tacccagggt cttagacatg aagtagtcct
     1981 tggtgtcaga aatgatcacg tccttgctaa actgcaggca cttcatattt tcggcttcag
     2041 tcaccaatgc ttcgctcatg ttgtggcctg tcaacgcttt cgtcaggtag taacctttgc
     2101 tcgcctcgca ttcctcggat tccagcatgt ccgcgtcctc aacttcggtc ttttgcacct
     2161 tagtctcgct ttcgttcagc agtttgctga taatagacct agacagctgc gggaagaccg
     2221 gggtcacgtc ttcaatatcc tcttttttac gctcagagct cagtttaatg atgcttttct
     2281 gcttcagaat gcactcatcc aacaagcact tcagttgctc ctcactcaaa catgaggtgc
     2341 tctccagttg gcccggcgta ttgccggtat gccacagcgc gttgcgcagg tgctcacccg
     2401 ggccggtggt gctgttaacc actttaacgg acaggcccga ataaccctgc gcgcgggtcg
     2461 ggttcgtatc ccaatagctc tgggtaacct gtttccacat gacgacgtag aaacgactcg
     2521 aagactggta accgaacacg aagccagcat aatcatcatc gcgctcggtg ttgatgaaaa
     2581 acgtgccgct gaagtcaacg gcattaaact catcgtatcc cacagccaga cccgggtcgc
     2641 agttcaccgt ttgtactaac tctttaccct gatgacgaac aacccagtta ggatcattct
     2701 gggacgtgcc tttcgggtcc agcggaatca tctggaagcg ccgaaaatcg gtttcgctga
     2761 tgtccacgtt ctcagggcag atgtcgtcga tgtccggcac cgaatcgtga tcgaagtcat
     2821 ccttgcacgc atccccacga ccgtcgccgt cagagtcttt ttgatctggg ttcggcacca
     2881 gacggcagtt gtccttatcg tccggaatgc catcgttatc gtcatcgtgg tcacacgcgt
     2941 cgcctttacc atccttgtcg tggtcggcct ggttcgcatt cggaacgtac gggcagttat
     3001 ccaagttatt ctgatggcca tcttcatcga tatcctggtt gttgtcacag gtatcgccga
     3061 ttcggtctga gtccgaatcc agttggtccg ggttgtgctc taacgggcag ttgtcgcatt
     3121 gatcaccaac accgtccata tcggtgtcac gctgatcaac attatagacg tactggcagt
     3181 tgtcacgttc gttgaggatg ccatcaccat cgatgtccgc tgcgcacgca tcaccttcac
     3241 cgttgttgtc ggtgtctgct tggtccggat tgtggttgta cgggcagttg tcgcagcggt
     3301 caccaacatc atcgcgatcg tagtcatact gcgcggggtt atagtggaac gggcagttat
     3361 cacgatcgtc tggaatttta tcgttgtcgt cgtcgtcgtc gcaagcgtct ccgatcccgt
     3421 ctttgtcgta gtcctcttgg ccgctatttg gcagattcgg gcaattgtcc ttcttacagt
     3481 gataggtagc attggccacg cataccaggt tctcattggg ccagccatcc aggtcggtat
     3541 cttctccaca gataatacca ttacccgcgt agcctggctt acattcacaa cgatacatcg
     3601 ggtcgctata atgacccagg tagttgcatt tagcgttctt gttgcaatca tgggtgccat
     3661 ccgtgcacgg gttacgcggc ttgcacacct gcttatttgc ggtggcgtgc tcgactcctt
     3721 ggccgaacgg ctgggaaccg gtgaaccttg gagggcacgg taaacaattg taacccgggt
     3781 cggtattttc gcaacgatgc tcgccgttgt ggttgaagca ggcatccgga acctccttgc
     3841 attcatccac atcggtgcac tggataccat taccggagta acccggtgga cacgcgccgc
     3901 atttccaact gccatccgga taggacgtgc acttgacacc tgcaaaacac gggttagaaa
     3961 gacaaccgtc gatagggcaa tcttgtttat tgcaaatctg gttctcagtc acgtcaccca
     4021 cacagtcttt gccaccaaac tgcggagttg ggttgttaca cagacggcta cgcttttgta
     4081 cgccaccgcc gcacgtaacg ctgcaaatat cccacgggct ccacggaccc caacctccgt
     4141 taatcgggca cgcgtccttt ttgcacgcct tggtttcacg ggcctcacct tcacacggct
     4201 tgccattcat ttgcggactc ggggagttgc acagacggat acgggttatc actccatcgc
     4261 cacaggtaac ggaacagctg ctccacggcg accaatggct ccaaccacca tcctgtttga
     4321 aacgtttatc gcattcttga atatggcagg tgcgggtctg aacgctggag ccctcgcagc
     4381 ggttattcag gctatcacag gaacggccac gctgctggat accattgccg cacgacgtgc
     4441 tacagctggt ccattcgctc cacggcgacc agccatcatc agcagaatcg ctcggccaac
     4501 aacgcgggca gcattccccg tccgggacgg tcgcgttgct gcatggcata atcgggcagc
     4561 tcaccttctt acagatcgta acgctgttct gacaatgaca ttcggtgcag gaatccaccg
     4621 tccattcttc attgttacga tactgtacgc cgttatgata gcacaacggc ggacgacgca
     4681 actcgttcgc aagctccttg ttctcctcgg taactttacg gatgctatct tgcagcgtgg
     4741 tcacaatcgt acgcagaccg cgcagctcca gaaccataga agagagttca tcgcagctga
     4801 taccacaaat cgcttgcaag tccttggttt tgtggccaat ataatttgta cggatcgccg
     4861 gggatgagcc attaacaaca ttgttgtcca gggtcagcag aacagaggta gaggagctac
     4921 agcctttgtt acgcagaatg tcttccgggg tggtaccaaa tacgaagcgc acattctgca
     4981 ggacgccttg aaagttatcg ttcacaccac cttttgcgat gcgcagacgg gcgatggacg
     5041 ccaaatcacg ggtgaaaacg ctttggatcg gcacatcgag ttcagcgttt tccatttttt
     5101 cacaatcgat atacaactgc gcgcggtcct cctgcacaaa caaggtgatg gacttccatt
     5161 gaccggtcgc cagtaacgcc tcttccacgg acaccacgtg ttgcttgcct tgcacggtca
     5221 ggctcaggtc cagagtaccg gccttaccgt tgctaacaac gctgaaaact tgaccgctgt
     5281 ggtccttgcg ctccaaagcc agcaaagtgc cgcgagtttt tttcatctgc cgcaggctag
     5341 cgaggagcag aaaacctttc tccgcgcgaa ccgcgtccac cagatcttga aacttgtcgt
     5401 ccggaaccgg cggaatcaag ttcgcatcct caatacgaaa cgccgggctg ctcgggtccg
     5461 gacctttcac gagacgacga ccgctgccct tgcgagctgc gccggtcaat tcaaagatat
     5521 caaagacgct attatcacca ccgctctccg ggatacgatt ggtaccgcaa acgtgcatca
     5581 ggaacaatac ccctagaccc caagctaatc ccatagcggc cgcacttccc ccggagcctc
     5641 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     5701 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     5761 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     5821 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     5881 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     5941 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     6001 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     6061 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     6121 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     6181 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     6241 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     6301 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     6361 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     6421 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     6481 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     6541 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     6601 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     6661 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     6721 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     6781 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     6841 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     6901 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     6961 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
     7021 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     7081 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     7141 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     7201 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     7261 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     7321 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     7381 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     7441 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     7501 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     7561 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     7621 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     7681 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     7741 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     7801 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     7861 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
     7921 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
     7981 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
     8041 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     8101 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     8161 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
     8221 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
     8281 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
     8341 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     8401 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     8461 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
     8521 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     8581 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     8641 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     8701 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     8761 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     8821 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     8881 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     8941 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     9001 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     9061 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     9121 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     9181 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     9241 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     9301 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     9361 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     9421 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     9481 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9541 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9601 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9661 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9721 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9781 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     9841 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9901 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     9961 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10021 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10081 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10141 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10201 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10261 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10321 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10381 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10441 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10501 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10561 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10621 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10681 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10741 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10801 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10861 tgccagtggc gataagtcgt gtcttaccgg
//
